Sol-gel

8 µl MgCl2, 0.7 µl dNTPs, 1 µl of each primer and 0.5 U Taq DNA polymerase. Then 5µl of products were incubated with 0.4U of Taq1 restriction enzyme at 65 °C for 48 h. Finally the products of the Taq1B digestion were electrophoresed on 1.5% agarose gel. For -629C/A polymorphism, 5µl of PCR products were incubated with 0.4U of Van91I restriction enzyme at 37 °C for 48 h. The thermocycler conditions after optimizing the technique for -629 C/A were: initial denaturation at 96°C for 5 min followed by

purpose of this experiment was to isolate two different proteins, from an availability of three (Myoglobin, BSA or Cytochrome C) using an ion-exchange chromatography method via a cation (CM) or anion (DEAE) exchanger. This was followed by a SDS-PAGE gel electrophoresis technique to determine the original mixture’s proteins and concentration with the help of a Bradford assay, which utilizes a Coomassie dye to bind to proteins. After all techniques were performed, Protein 1 was found to have an absorbance

using the *** as the blank. Day 3: Protein Characterization Using SDS-PAGE Gel Electrophoresis and BCA Assay A crude whey sample and a purified *-lactalbumin sample were prepared for SDS-PAGE gel electrophoresis by mixing 20 µL of each sample with µL of the reducing gel buffer in Eppendorf tubes. These two samples were then boiled for 5 minutes and then allowed to cool to room temperature. A precast gel was inserted into the gel running apparatus and the comb was removed. Consequently, a 10x stock solution

Figure 1 contains gel electrophoresis for protein samples. The lanes were labeled from 1 to 10 from the right to the left. Lane 1 contained the ladder fragment. Lane 2 contained the filtrate. Lane 3 contained the S1 sample. Lane 4 contained the P1 sample. Lane 5 contained the P1 medium salt sample. Lane 6 contained the P1 high salt sample. Lane 7 contained the S2 sample. Lane 8 contained the P2 sample. Lane 9 contained the P2 medium salt sample. Lane 10 contained the P2 high salt sample. Table

For this experiment, whole bovine blood was used. The first process was to separate the blood into cellular and plasma fractions. 100 µL of whole bovine blood was transferred into a yellow microcentrifuge tube that was labeled WB using a P-200. 50 µL of whole blood was added to a blue microcentrifuge tube labeled WB. Both tubes were capped and placed in ice. 2 mL of the remaining blood was transferred into a Clear 2 mL tube using a P-2000 and centrifuged for 5 minutes at 8000 RPM. Afterward, 800

The following results helped obtain the haplogroup that in which the sequence of mtDNA would identify. The PCR reaction worked, and this can be determined by looking at the agarose gel in figure 1. If the PCR reaction was successful, than a band should appear around 550bp. Individual AC displays a band around 550bp, this means the PCR reaction was successful. The band for individual AC, depicts a low concentration of product, because the band faint. After the purification process the concentration

Agarose is a gel that comes from seaweed extract. The polysaccharide polymer does not provide uniform pore sizes, but it does well with molecules that exceed a size of 200 kDa. It also is often used for DNA strands that have more than 50 base pairs. So, an agarose gel electrophoresis is used when separating fragments of DNA and RNA by size. The gel acts as a sieve, causing the larger fragments to travel more slowly and less distance than the smaller fragments. Before making the gel, some important

polarity. Caffeine, the more polar molecule had a greater affinity for the polar silica gel stationary phase causing it to consistently have a lower retention factor regardless of the mobile phase. This methodology can be effectively used to distinguish and analyze the polarity various of chemical mixtures such as within medicines, inks, etc. In thin layer chromatography a stationary phase, silica gel with a glass backing, is dotted on a pencil drawn

AGAROSE GEL ELECTROPHORESIS I. INTRODUCTION Agarose gel electrophoresis (AGE) is the most common and effective way of separating and analyzing DNA fragments of different sizes (Lee & Costumbrado, 2012). The agarose gel, at the time that it is completely polymerized, acts as a molecular sieve that, in the existence of electric current, pull apart DNA by their molecular weight and size. AGE is not mostly used to visualize DNA but also used for quantification or to single out a particular band. The

and is used to distinguish from different species based on variation, commonality, or evolutionary divergence. First, proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according to size using an electric current. Proteins that are separated after are blotted from the gel and onto a paper membrane. An antibody is then added to the membrane paper and causes a colored reaction. Following the reaction, the results