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10-Touch Test Lab Report

Satisfactory Essays

Mutagenizing C. elegans- place agar plate containing C. elegans 50 cm way from 40 W UV lamp. Remove the lid and turn on the lamp for 15 minutes. Creating the gentle touch probe- Obtain an eyebrow hair and glue the root end of this hair to the toothpick. 10- touch test- Lightly drag the hair cross edge of the pharynx(head) and the tail. Alternating touch between head and tail gently stroke the worm 10 times. Record the worm’s response “1” equates to a response and “0” means no response. Refer to table 1 for detail about quantifying a response. Preform this test on 10 nematodes per strain. Before recording results make sure to practice the stroking technique; mec train should respond 30-40% of the time and the wildtype should respond 80-90%

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