Mutagenizing C. elegans- place agar plate containing C. elegans 50 cm way from 40 W UV lamp. Remove the lid and turn on the lamp for 15 minutes. Creating the gentle touch probe- Obtain an eyebrow hair and glue the root end of this hair to the toothpick. 10- touch test- Lightly drag the hair cross edge of the pharynx(head) and the tail. Alternating touch between head and tail gently stroke the worm 10 times. Record the worm’s response “1” equates to a response and “0” means no response. Refer to table 1 for detail about quantifying a response. Preform this test on 10 nematodes per strain. Before recording results make sure to practice the stroking technique; mec train should respond 30-40% of the time and the wildtype should respond 80-90%
In the second part of the experiment, they took 3 test tubes, one containing a small amount of potassium chloride, one with a like amount of potassium chlorate, and one that contained a solution obtained from adding distilled water to the crucible, that was used in the first part of the experiment, and heated it for about a minute, and then they added 10 ml of distilled water to each and stirred. In each of the tubes, they added 5 drops of dilute (6 M) nitric acid and 5 drops of 0.1 M silver nitrate solution, then stirred each test tube and observed carefully.
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
Make three exposures using given technical factors on a phantom knee in PA position . Include saline bags in exposures 1 and 2 to demonstrate patient soft tissue thickness.
Fig. 12 CXL10-/- mice are relatively protected against FFC-induced liver injury and inflammation. WT & CXCL10-/- mice were fed either chow or FFC-diet for 20 weeks. (A) Plasma alanine aminotransferase (ALT) levels were measured. (C) Total RNA was extracted from liver tissue and mRNA expression of surface macrophage marker cluster of differentiation (CD)68 was evaluated by real-time qPCR. (D) Assessment of macrophage infiltration in fixed liver tissue was done by immunohistochemistry using macrophage galactose-specific lectin (Mac-2) antibody. Bar columns represent mean ± S.E.M. *** P < .001, * P < .05 compared to WT chow-fed mice.
Begining by labeling 7 different 2.0 ml tubes 0 thru 6 for each compound. Then add 1ml of extract to tube 0. Then add 0.5 ml of DMSO to tubes 1 thru 6. Now make a 1:2 serial dilution from 0(pure extract) to 6(1:16)
In this project, C. Elegans are hermaphrodite worms that will be used since they are easy to maintain in lab, as well as have short life cycles. The gene that the project attempted to knockdown in C. Elegans with RNAi treatment is the unc-22 gene. RNAi disrupts gene expression in the presence of double stranded RNA (dsRNA) that is complementary to target gene sequence. The unc-22 gene codes for a muscle protein called twitchin in wild-type worms. The Unc-22 is required for muscle regulation and maintenance in C.Elegans. To verify that the RNAi treatment worked, would check the unc-22 mRNA levels in the worms, in addition to phenotype observation.
The experiments conducted for this lab report focused on water contamination and filtration. Experiment 1 was effects of groundwater contamination. Oil, vinegar, and laundry detergent were added to clean water with no means of filtration. The clean water was found to be contaminated. A filtration system consisting of cheesecloth and 60 ml of soil was created and the contaminated samples were filtered through it. The soil and cheese cloth did not affectively filter the contaminants. Experiment 2 focused on
2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.
The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect
The mean voltage of the battery terminals while connected to the identification resistors is presented in Figure 4 12. These samples have been pulled out from the voltage sensor of the PEB panel. The voltage decreased as expected from 12.53 to 12.5 during first 20 seconds of connection to the
In the method of continuous variations the total number of moles of reactants is kept constant for the series of measurements. Each measurement is made with a different mole ratio of reactants. A mole ratio is ratio between the amounts in moles of any two compounds involved in a chemical reaction. Mole ratios are used as conversion factors between products and reactants in many chemistry problems.
Oxidation State: Oxidation state or oxidation number (O.N.) is the apparent charge assigned to an atom of an element in a molecule or in an ion. Valency: Valency is the combining capacity of an atom with other atoms. It depends upon the number of electrons in the outermost shell. Q. 9: Calculate the oxidation number of Nitrogen in HNO3.
My boss then proceeded to run the gel for 30-40 minutes at 100 volts. This is the perfect amount of time for electrophoresis to be completed.
The two independent variables were luminant cue patches (light cue, dark cue and equiluminant cue) and location of the cue and target (valid side with cue and target on same side and invalid side with cue and target on opposite sides). The dependent variable was participants’ reaction time in millisecond.
The purpose of these series of experiment was successful as seen from the results. The recombinant form of the green fluorescent protein was successfully expressed and purified from E. coli. Though a high yield and purity were not obtained, the qualitative monitoring of the rGFP activity was consistent with the quantitative values obtained. Qualitatively, the elution fraction E2 fluoresced the most when placed under a hand-held UV lamp. Quantitatively, E2 also had the highest activity with 41900 RFUs when read by a fluorescent microplate reader. One way to increase the yield of rGFP would be to increase the IPTG induction time. This would increase the amount of rGFP that is expressed and consequently increase the amount of rGFP that is purified. There were other things during the experiment such as contaminant proteins that could also have affected the yield of rGFP obtained.