Unit 9 Final Lab Report

Procedure: I used a ruler, thermometer, and scale to take measurements. I used a graduated cylinder, short step pipet, scale, and ruler to determine volume and density. I used a volumetric flask, graduated pipet, pipet bulb, scale, and glass beaker to determine concentrations and densities of various dilutions.

C. Pour about one quarter of the first unknown packet into the first cup and add

To begin, three sets ofabout 0.3000g of KHP are weighed out on an analytical balance. Put the three sets of KHP into three separate, labeled flasks. All three sets of the KHP is then dissolved with approximately 50mL of deionized water. Next, a buret is used to start the actual titration. Buret is initially filled to 0.00mL mark with the NaOH solution, this is recorded as initial volume. Next, add 2-3 drops of phenolphthalein indicator into each of the three flasks containing KHP. A magnetic stir bar is then added to the first flask, and placed above a stir plate. Everything is positioned under the buret. Stirrer is put on medium speed and the titration can start. Slowly release the NaOH into the KHP flask. As the end point is reached, a pink color will be seen in the flask. When the lightest pink possible remains in the solution for more than 30 seconds titration is complete. The final volume is recorded, and the same steps are taken for the other two sets of KHP solution. Finally, blank titration is completed to determine deviation.

2.) Add 20ml of dichloromethane, gently shake to extract, be sure to vent by opening the stop-cock. First extraction successful.

1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.

Sequentially, add 100ul from the previous sample to the next e.g. take 100ul from the neat sample dilution into the following 900ul forming 10

Remove foam plug from the inoculate flask and pour it into a cuvette. Incubate it at 37°C and the optical density readings at 600nm every 10-15 mins. Pour into the

Then, in spot plate 1, we used solution A to place 1 drop of it in well 1, 2 drops in well 2, 3 drops in well 3, up to 10 drops in well 10. Next, in spot plate 1, we added 9 drops of water in well 1, 8 drops in well 2, 7 drops in well 3, up to 1 drop in well 9. In this way, each spot on plate 1 had a different concentration of iodate ion. Then, in spot plate 2, we added 10 drops of solution B in 10 of the wells.

3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate.

Add exactly two microliters of each sample and standard to their corresponding marks on the plate. Place the bottom centimeter of the plate into a glass jar with approximately 500 microliters of ethyl acetate. Allow the ethyl acetate to be absorbed up the plate until three centimeters from the top of the plate. View the plate under a UV light and mark the movement of each analyte with pencil. If the analyte is in the same position as the standard BMK, place the test tube’s solution

Add the mixture by placing your pipette against the wall of the column so as not to disturb the bed. The mixture was allowed to enter the bed, close to the bottom the bed (about 1 cm) by opening the stop cock . Then, about 15 mL of buffer was added into the column. 3mL of buffer added slowly each time to prevent the overflow and avoid disturb the bed. 1 mL fractions were collected into each cuvette until there is no color present in the last fraction.

6. Remove 100 ul of the solution in the pH 4 test tube every 30 seconds,

Lab procedure: prepare for agar solution, make the gel with wells on one side. Put the gel in the water into an electric field, wells on the negative side. Collect

The sample will be transfered to a 1ml LB media, put in a shaker at 37c and 300rpm for 20min

Collect to 2 large beakers both large beakers are to be filled with hot water (labtutor). Then obtain seven conical tubes these will be used to collect the levels of gas, you will also need test tube a stopper and a plastic tube (labtutor). You want to fill the conical tube to at least 50 ml of water (Cressy). Take the four conical tubes filled with water and place two in each beaker, to do this you must invert the tube and cover the release hole as to not lose any water (Cressy). Then place the beakers with the tubes in the bath so they can be at the same temperature as the bath (Cressy). Next mark all of your test tubes in number order to be sure which tube contains what concentrations and pH (Cressy). Having mixed a solution to the specifications of 2.5 ml of glucose in all tubes, 3 ml of yeast in 2 tubes of pH 5, 2 tubes of pH 9, and the single pH 7 tube, the remaining two tubes will contain no yeast as they will be negative controls. Next add 2 ml of pH buffer 3 tubes will receive pH of 5, three will receive a pH of 9 and a single tube of pH 7. Finally add pure water to make sure all test tubes have 10 ml of solution. When making the solutions