Drosophila melanogaster stocks used for the behavior assays (2-choice assay and tracking assay) and molecular analysis (qRT-PCR and immunohistochemistry), include the wild type Canton-S (CS) line, the UAS-GABA (B)-RX-RNAi (Root et al., 2008) (where X represents receptor subtype 1, 2, or 3), and Or X-Gal4 lines (Or 47a Gal4 and Or 42b Gal4), GH-146 Gal4, Orco-Gal4, 10x; UAS-CD8; GFP were purchased from the Bloomington stock center (http://flystocks.bio.indiana.edu). Virgin female flies
Abstract Drosophila melanogaster are a great species for students to learn the process of Mendelian inheritance. They reproduce rapidly and have distinct phenotypes that are easily observable under a microscope. The experiment involved anesthetizing, observing and categorizing these flies based on their wild type and mutant phenotypes to figure out the mutant phenotypes mode of inheritance. We hypothesized that for the mutant vestigial wings phenotype, the mode of inheritance was autosomal dominant
century was a time of change for many fields of science, but in terms of genetics, Drosophila melanogaster rose as one of the most significant organisms to use as a model for scientific findings to come. Drosophila melanogaster is a small fly, approximately 2 mm in length that feeds on old, decomposing fruit. Over its 100+ years as a model organism, two decades, 1910-1920 and 1970-1980, proved to hold Drosophila melanogaster in utmost importance. Thomas Hunt Morgan, who did most of his work at Columbia
be studied on a multitude of organisms, some more than others. Drosophila melanogaster or the common fruit fly has been studied in depth for its great advantages, such as size, reproduction rate, ease of care and inexpensive room and board. In this experiment we tested to see what the offspring of an unknown cross of an F1 generation would produce. After observing the F2 generation and recording the data we found some of the Drosophila showed mutations, two in particular. The mutations were the apterus
A Study Of Inheritable Traits in Fruit Flies INTRODUCTION The Drosophila melanogaster, more commonly known as the fruit fly, is a popular species used in genetic experiments. In fact, Thomas Hunt Morgan began using Drosophila in the early 1900’s to study genes and their relation to certain chromosomes(Biology 263). Scientists have located over 500 genes on the four chromosomes in the fly. There are many advantages in using Drosophila for these types of studies. Drosophila melanogaster
A Study Of Inheritable Traits in Fruit Flies INTRODUCTION The Drosophila melanogaster, more commonly known as the fruit fly, is a popular species used in genetic experiments. In fact, Thomas Hunt Morgan began using Drosophila in the early 1900's to study genes and their relation to certain chromosomes(Biology 263). Scientists have located over 500 genes on the four chromosomes in the fly. There are many advantages in using Drosophila for these types of studies. Drosophila melanogaster can
Mode of Inheritance: Drosophila Melanogaster Hannah Hill BIO 2450: Genetics Lab Instructor: Payton Prather Abstract Transmission genetics is a very important area of study in which specific traits or mutations are analyzed to find how they are passed on to future generations (Russel 2009). This form of genetics, founded by Gregor Mendel, categorized how genes are inherited by discovering specific ratios in which phenotypic traits are expressed (Russel 2009). Mendel’s work served as the basis
The Effect of Different Frequencies of Light on the Number of Cristae in the Mitochondria of Drosophila melanogaster Malavika Pillai 09/22/16 Introduction Rationale Statement of problem: My topic aims to investigate the effect of different frequencies of light on the number of cristae in the mitochondria of Drosophila melanogaster. Cells, tissues, organs, and an organism all depend on healthy and efficient mitochondria which fuel essential metabolic reactions and provide energy
Q1: Drosophila melanogaster or fruit fly in general terms, was one of the earliest organisms that were used for genetics analysis by scientists. The reasons behind the use of this species are due to its small size, short generation time and the ability of females to lay a lot of eggs. Since Drosophila melanogaster has been studied in 1900, this have brought a large contribution to genetic studies and particularly used as model organisms in the Human Genome Project. In recent years, the whole genome
certain species (Drosophila melanogaster) displayed throughout different generations. For this experiment to be carried out, Drosophila melanogaster, also known as fruit flies, were used to visually represent evolutionary conceptions such as Hardy-Weinberg equilibrium equation. At the beginning of the experiment, the parent generation was observed first. Throughout the course of seven weeks, the vial was analyzed for certain changes between the two populations of Drosophila melanogaster; wild type and