Anti Inflammatory And Macrophage Inhibitory Potential Of Inflammation

2462 Words Mar 9th, 2015 10 Pages
Anti-inflammatory and Macrophage Inhibitory Potential of Picrorhiza kurroa in experimental models of inflammation.

ABSTRACT
The rhizome of Picrorhiza kurroa Royle ex Benth, is widely used for the treatment of various inflammatory conditions. In the present study, we have evaluated the anti-inflammatory and anti-granuloma activity of Picrorhiza kurroa rhizome extract (PKRE) against carrageenan-induced paw edema, cotton pellet implantation induced granuloma formation and Complete Freund 's Adjuvant (CFA) induced stimulation of peritoneal macrophages in experimental rats. The anti-inflammatory activity was grossly observed by determining the efficacy to inhibit the increased paw edema volume while the anti-granuloma activity was evaluated by measuring the dry granuloma weight and serum pro-inflammatory cytokine level. Furthermore, protein expression of important mediators secreted by macrophages namely TNF-R1, IL-1β, IL-6, IL-10 and COX-2 were evaluated in stimulated peritoneal macrophages by western blot to predict the putative mechanism of action of PKRE in treating inflammation. Treatment with PKRE produced dose dependent inhibitory activity in carrageenan induced paw edema and granuloma formation. PKRE treatment significantly reduced serum pro-inflammatory cytokine levels in cotton pellet induced granuloma model. Protein expression of inflammatory mediators IL-1β, IL-6, TNF-R1 and COX-2 were reduced significantly (P<0.01) in response to PKRE treatment. In addition, PKRE…
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