Association analysis of novel SNPs in exon 10 of growth hormone receptor gene with growth traits in Indian sheep
Amiya Ranjan Sahu*1, V. Jeichitra2, R. Rajendran3 and A. Raja4
Animal Genetics and Breeding, MVC, TANUVAS, Chennai, India
*Corresponding Author: dramiyavet@gmail.com
Present address:
1. Ph.D. Scholar, Animal Genetics and Breeding, ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., India
2. Associate Professor, Animal Genetics and Breeding, Veterinary College and Research Institute, Orathanadu, T.N., India
3. Professor, Directorate of Research, TANUVAS, Chennai, T.N., India
4. Professor, Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, T.N., India
Summary
Growth hormone receptor (GHR)
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Key words sheep, growth hormone receptor gene, polymorphism, tetra-primer ARMS-PCR
Introduction
Small ruminants are used by the human being in many ways that provides proteinous meat, induces the growth of farmers’ income, facilitates rural employment and improves fertility of the soil (Thiruvenkadan et al. 2011). As per 19th Livestock Census, India possesses 42 breeds of total 65.06 million numbers of sheep (Livestock Census Report 2012). The sheep population constitute 12.7% of the total livestock population of India and ranks 2nd in the world sheep population (FAOSTAT 2014). Total annual mutton production in the country is around 7.12%. The sheep population of Tamil Nadu is 4.47 million numbers which produces approximately 31440 tonnes of mutton (Anon. 2015). The efficiency of sheep enterprises can be uplifted by increasing litter size, lamb weight, mutton production and by the improvement in quality of wool. DNA based molecular markers are used efficiently for assessment of genetic diversity, selection of animals in early age, study of population structure, mapping of genes and quantitative trait loci (QTLs), and breeding based on genomic selection (Collard et al. 2005). The relationship between the economic traits and the genes associated with these are studied by the candidate gene approach (Andersson 2001). Marker Assisted Selection (MAS)
Achondroplasia is a genetic disorder in which there is a growth hormone deficiency, or there is a genetic mutation in either the father’s sperm or mother’s egg. (mayo clinic, March 20,2014.) Achondroplasia was the first discovered in ancient Egyptian records. People with achondroplasia were considered people with supernatural powers. Many people call dwarfs midgets but to them, it is very disrespectful because midget literally means little person. Some acceptable names that you can use that will not offend them would be little people, LP, person with short stature, or dwarf. Even though dwarfs feel as if they do not have disabilities, the Americans with Disabilities Act (ADA) will protect the rights of dwarfs. (who discovered it? March
i.When testing for hypothyroidism, why is the free T4 level an important measurement? Predict what the TSH and T4 test results would indicate in someone with primary hypothyroidism. Explain your answer. (100 – 200 words, 5 marks)
People are all extremely different. We all have different skin tones, eye color, hair color, height, weight, or even behaviors. Race could be referred to as a phenotype, or a person’s physical characteristics that we see. We are all certainly different, but we are also essentially the same. Many people only consider about phenotypic differences and make assumptions about specific behavior and culture based on their own ideas of different groups.
Farmers are using different technology for breeding. They used selective breeding to produce animals that exhibit desirable traits and they get more benefit from it. For example, using breeding techniques farmers makes cows which produce more milk with less lactose, and sheep which produce more wool. Farmers accept this new selective breeding technology because in the past, farmers would use growth hormones to promote such qualities. This became problematic when residue of the hormones remained in the meat, leaving it with a foul taste. When researchers began to clone transgenic animals, it became possible to develop certain traits in animals, which increased the quality of their yield. When a farmer would like to raise the standards of a herd, the breeding process is very slow and sometimes incomes can decrease (Wilmut 23). Many times when relying on sexual breeding alone to mass-produce these animals, there are chances of breeding out the desired traits (Freudenrich). Transgenic animal cloning will result in higher quality meats and dairies without the use of artificial hormones. The U.S. Food and Drug Administration released in January 2008 concluded “edible products from normal, healthy clones or their progeny do not appear to pose increased food consumption risks relative to comparable products from conventional animals.” After 2008, US Food and Drug Administrative agree to use
It is believed that sheep evolved in the dry, mountainous areas of Southwest and Central Asia around 8,000-10,000 years ago. The fibers, milk, hide, and meat provided by sheep continue to prove just how essential the animal is to both temperate and tropical agriculture. They are also very important to the United States because we have bred them for three major purposes: the production of very fine wool for clothing, the production of long wool for heavy clothing and finally for the production of mutton and lamb. Sheep have been bred to produce these three major necessities in such a short time that we don't consider the genetic downfalls, such as inbreeding. If that were the case, inbreeding would keep producing all of these major productions
The lipoprotein gene mutation has a penetrance of greater than 90% (Henderson et al., 2016). Heterozygosity takes place when an individual has two dissimilar alleles of a gene. A single dominant gene can manifest itself in a heterozygous person particularly when there is high penetrance property. This is the reason why multiple generation can be affected by the FH disorder as seen in the proband and family members of the followed case discussion. Monogenic FH is largely attributed to mutations in the LDL-receptor (LDLR), apolipoprotein B (APOB), and proprotein convertase, subtilisin/kexin-type 9 [PCSK9] genes (Brautbar et al.,
I’m sure a lot of you know very little or nothing at all about cattle genetics and breeding, or genetic improvements. Cattle genetics is a very long process that is used to improve your
The dramatic increase of people on planet earth over the span of 5 decades has raised many questions about food production and how much is needed to sustain the rising population. Furthermore, the increased productivity of animals, specifically the growth and processing of animals is now needed more than ever in order to support the upcoming years. Moreover, the means to combat this problem is the use of genetically modified animals. Genetically modified animals can contribute to the increase of animal productivity as there have been many examples of animals which have greatly accelerated growth periods, which decreases the amount of time it takes in which an animal can be sold as a food product. Additionally this also cuts the amount of food they consume by a significant margin, further increasing their efficiency. One example of this is the AquAdvantage salmon, which has a growth hormone gene from Pacific Chinook salmon and a switch on gene from ocean pout. The AquAdvantage fish grows at twice the rate of normal Atlantic salmon due to its ability to grow all year round, and at an increased rate. Through these advantages alone, anyone can see the incredible value and importance of genetically modified animals have over normal
Are you trying to build muscle, gain muscle weight, and look more attractive? growth hormone supplements are safe, affordable options. These are supplements that are designed to make you look better, build muscle, and be happier with your level of fitness. A growth hormone supplement is something that you can take to get real results and it's accomplished by taking something that is very safe for your body. You can find growth hormone for sale online, there are lots of different websites that specialize in these products.
As scientists are experimenting with many methods of gene selection, the livestock industry are relying more on genetic selection. Genetic selection allow the farmers to produce the most fertile and efficient livestock. There are many impacts of genetic selection. Genetic selection allows the farmers to genetically modify the breed of the livestock to that mass production can occur to fulfill the need of consumers. With genetic selection the quantity and quality of meat and dairy production has been increased. I was not familiar with the technique of artificial insemination for reproduction biology until I took this class. I learned that artificial insemination is also a technique of genetic selection which allows the scientist to decide what kind of livestock they want. Also, to improve in fur quality of animal, the genetic selection is in used. With genetic selection, scientist can now improve the production rate of cattle. Genetic selection can also improve the physical characteristics of the livestock. To keep up with the world’s need in beef and dairy production genetic selections have a huge impact. Nowadays, the agriculture industries have been vastly improved because of the advancement in the genetic selections. Genetic selections also allow the farmer to modify animal more for either meat or dairy production. For example, Boer goat has been genetically modified to gain
Human growth hormones. What is really know about them? The average person probably never give human growth hormones a second thought. Accordingly, they are what help children to grow. The internal workings are developing to be viable and healthy organs. By the time a child reaches puberty, the production of HGH comes to a peak. It's not until he or she turns a quarter century that the HGH levels winds up being at or around 600ug. This number greatly decreases to equal less than 20% of that 600ug.
Aim 1. Resolving the haplotype architecture of GHRd3 to determine its functional and evolutionary impact: In our preliminary studies, we have now resolved the haplotype architecture using focused analysis of thousands of genomes. This will allow us to thoroughly interrogate associations of different GHR haplotypes with metabolic and developmental diseases, expression and methylation and levels of neighboring genes in different tissues. In addition, using population genetics and model-based analysis of the newly resolved haplotypes will enable us to trace back the adaptive pressures acting on the locus, shedding light on the evolutionary forces maintaining this deletion in the population.
In size, it is nothing special. In function, though, it is critical to your health. The pituitary gland (hypophysis) is a pea-sized endocrine gland lying in the very center of your skull. It controls vital functions, ranging from the amount of stress you feel, to your physical growth and reproduction. The nine hormones secreted by the pituitary keep you in balance – and the most abundant of these secretions – human growth hormone (HGH) – may serve to preserve youth … if only you could find a way to increase your HGH levels.
Isolate genomic DNA from WT animal (want WT version of the gene) to be used as a template
Human growth hormone, a substance produced in the human body, is now being sold over the Internet. Companies are marketing the product as an anti-aging product. At the web-site, http://www.awakenhgh.com, the company claims that there is a possibility that one can "stop the aging process." On the home page, they claim that the product can reduce fat and cellulite, increase energy and muscle tone, elevate mood, improve sexual performance and sleep, remove wrinkles, balance cholesterol, re-grow and re-color hair, and more. The product is a dietary oral spray that they claim contains a product that is "identical to the growth hormone manufactured in the human body" and is designed for oral consumption. They also