The peptide antibiotic Bacitracin:
Bacitracin is a cyclic polypeptide antibiotic that was first discovered in 1945 by Johnson et al. and is produced as a mixture of different similar peptides by certain strains of Bacillus subtilis and Bacillus lichenformis. The main compound is bacitracin A and its structure can be seen in Figure X. (Johnson 1945, Strominger 1971, Konz 1997). It is produced nonribosomally by a multienzyme complex composed of the three bacitracin synthetases BA1-3 by a so called thiotemplate mechanism. In this mechanism, adenylated amino acids are activated by hydrolysis of AMP and added to the enzyme, where it is bound as a thioester on the covalently bound 4-phosphopentatheinyl-cofactor. The polypeptide is then produced in a series of transpeptidation reactions (Konz 1997).
Figure X: Sequence and structure of Bacitracin A. The amino acids from L-Lys to L-Asn form the heptapeptide ring and the second cysteine forms the thiazoline ring with the backbone.
The mechanism by which bacitracin inhibits cell growth is by targeting the cell wall synthesis. This is achieved by binding to undecaprenol-pyrophosphate (UPP, C55-PP), which functions as a lipid carrier of peptidoglycan (PG) intermediates during the synthesis of the cell wall, transporting the intermediates from one side of the membrane to the other.
…show more content…
The cycle starts with UP, which is produced by dephosphorylation of UPP either through recycling or from de novo synthesis. UPP is then coupled to activated MurNAc-pentapeptide by MraY and activated GlcNAc is added by MurG in a second step to form Lipid II. This is transported across the membrane by the flippase FtsW and the GlcNAc-MurNAc-pentapeptide is transferred to the growing peptidoglycan chains. The UPP in the outer leaflet has to be dephosphorylated and transported back to the inner leaflet for the cycle to complete. Bacitracin binds to UPP and inhibits
In a study by Jun Lin et al., it was stated that bacitracin is an antibiotic that has a growth-promoting ability. This means that it would have no affect on inhibiting, or stopping the growth of bacteria, in particular, E. coli. On the other hand, a study done by Sukayna M. Fadlallah et al. proved that gentamicin proved a promising ground for treatment of E. Coli. Different molecular strains of E. coli were subject to different regimens of antibiotics, including gentamicin. The results
My coworker had a 40-year-old patient who came to the hospital for alcohol withdrawal. All the nurses on our floor knew him really well because he visits our hospital frequently. At around 7:30 pm, our telemetry showed that the patient was having a heart rate of 180s to190s. We all were still getting report. Charge nurse went to assess the patient, patient started yelling in a loud voice, throwing pillows and a blanket. It turned out that the patient went to the delirium tremens (DT) phase. He was confused, disoriented, hallucinating, agitated, irritated and had muscle tremor. When reviewing the medication administration record (MAR), charge nurse noticed that CIWA scale was not done as ordered and as a result patient did not get enough Lorazepam
The data from the results supports the product that was synthesised was 4-Bromoacetanalide, this was proved by various factors. For example, the literature range for the melting point of 4-bromoacetanalide was 166-170 whereas the average melting point recorded from the final product made was 169.2°C, which fits within the range. Melting points are also sensitive to purity which shows the product obtained was of a pure nature as the product melting point is sharp within the range. Another way in which the product can be proved to be 4-bromoacetanalide is by the IR spectrum. The spectrum shows many peaks for different functional groups, which are found in 4-bromoacetanalide. These include the peaks for the secondary amine (N-H) at 3303.1cm-1,
In vitro: A non-toxic concentration of enniatin B could strongly inhibit a Pdr5p-mediated efflux of cycloheximide or cerulenin in Pdr5p-overexpressing cells. The mode of Pdr5p inhibition caused by enniatin B was competitive against FK506. However, enniatin B could not inhibit the function of Snq2p, a homologue of Pdr5p [1]. Another study showed that enniatin B was a relatively poor ionophore that could facilitate import of K+ and Na+ across membranes [2]. It was also found that like other enniatins, enniatin B was able to inhibit acyl-CoA: cholesterol acyltransferase [3].
Enoxaparin is a low molecular weight heparin used to prevent thrombosis, particularly post surgery. Enoxaparin binds to antithrombin III which is responsible for inhibiting coagulation by acting on factor Xa. Enoxaparin accelerates the activity of antithrombin III therefore preventing clot formation (McKenna & Lim, 2014 p766). When a blood vessel is damaged, platelets in circulating blood stick to the site of injury and release chemicals that attract more platelets causing aggregation. There are both intrinsic and extrinsic pathways causing thrombi to form to maintain a closed cardiovascular system (Hollar, 2017). Immobility increases risk of thrombus formation as the blood becomes stagnant with gravity and decreased
In 1995, the FDA approved a miracle drug, which would aid in a person's ability to cope with the severe pain associated with cancer. Purdue Pharma L.P. of Stamford, Connecticut, introduced the wonder drug that would eventually be the demise of many. Oxycotin would, for several, lead to addiction, criminal behaviors, and, for some, their lives. The intent of releasing the drug was solely to treat patients suffering from chronic pain. Since the release of the drug, doctors are now prescribing the medicine for moderate pain as well. Patients have become extremely addicted and have gone to extreme lengths to obtain the "poor man's heroin," which may include criminal activities. Recovering addicts endure an
How to Buy Gabapentin Pills Online, Buy Neurontin (Gabapentin) online, for antiepileptic treatment purchase Gabapentin Online, Buy Gabapentin Online without prescription, get Gabapentin coupon code
The most striking aspect of the financial overview of medicine was the limitations of the Affordable Care Act (ACA). Even before the lecture, it was clear to me that from the inception of the law that care accessibility would be prioritized over cost and quality. However, the lecture helped me realize how myopic my understanding of the consequences were for patients.
Uric acid is produced through the breakdown of hypoxanthine and xanthine, and this breakdown is catalyzed by the enzyme xanthine oxidase (XO). High uric acid levels in the human body leads to the formation of gout. Gout is commonly treated with allopurinol, a synthetic chemical drug. However, allopurinol elicits many side effects and induces adverse drug reactions. As such, alternative natural drugs have been introduced to reduce side effects. In this study, the XO inhibitory activity of Morinda citrifolia L. leaves were investigated to evaluate the efficacy of this herb as a substitute for allopurinol. Dried, powdered plant materials were subjected to separate extraction using three solvents, particularly 95% ethanol (EE), methanol (ME), and
Loratadine HCl (LOR) , which is chemically designated as ethyl 4 (8-chloro 5,6 dihydro,11H- benzo[5,6] cyclohepta[1,2 b]pyridin- 11- ylidene) -1- piperidinecarboxylate Hydrochloride is a tricyclic, piperidine derivative of antihistamines [1,2]. It is a second generation antihistamines, so it has non–sedating properties. Loratadine are newer drugs that are much more selective for peripheral H1 receptors as opposed to the central nervous system H1 receptors and cholinergic receptors. H1 antihistamines are applied in the treatment of allergies, they prevent symptoms such as itching, congestion, rhinorrhoea, tearing and sneezing. This selectivity significantly reduces the occurrence of adverse drug reactions, such as sedation, while
Doxycycline, a semi-synthetic derivative of oxytetracycline is a potent antibacterial drug commonly used as doxycycline hyclate (DOX-h) and used for both in humans and animals treatments [8]. The antibacterial mechanism is mainly regarded as time-dependant and DOX-h is a relatively inexpensive antibacterial drug, potent and possesses a broad-spectrum antibacterial action [9]. In this regard, a poloxamer-based matrix was used to produce a long-acting injectable preparation (DOXh-LA) and its serum concentrations vs. time profile was investigated after its s.c. injection to calves. It was found that the DOX-h-LA showed the greatest values for bioavailability (602%); maximum serum concentration (Cmax) of 1.99 lg/mL with a time to reach (Tmax)
The Action Plan synthesizes all the marketing decisions that were made up to this point into an effective plan for activation. New target segments were identified in order to ensure Aleve’s continued growth. For example, younger body pain consumers could be targeted with the same functional and emotional benefits as arthritis sufferers: ease of mobility and freedom to do what they choose. Liquid gel tablets were eventually advertised to these consumers as a modern product with fast pain relief that fulfilled their active lifestyle.
REVIEW THE PRODUCTION OF NAMED ANTIBIOTIC, NOTING BIOTECHNOLOGICAL INNOVATION THAT IMPROVE ASPECTS OF THE PROCESS
The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment
Bayan Telecommunications, Inc. (BayanTel) is a telecommunications company serving areas in Metro Manila, Bicol and local exchange service areas in the Visayas and Mindanao regions combined cover a population of over 25 million, nearly 33% of the population of the Philippines.