The idea of the experiments is to introduce the concept of bacterial multiplicity of the shapes, forms and species. In addition, the objective of this concept was to recognize, and observe bacterial diversity from three different experiments. The first experiment was to identify differential growth, the second experiment was to calculate bacterial population, and the third experiment was identifying which bacterium was Gram positive or Gram negative. Bacterial diversity has nearly 5,000 species of bacteria that is known, however, it is believed that there are still more types to be discovered. Due to bacteria of being small, it is difficult to culture them and study in precise detail. Although bacteria are small, they are not the smallest living organisms. Let alone, the identification of bacteria is based on a combination of characteristic in cell shapes. The three basic …show more content…
In most cases, bacteria are an important contributor to the ecosystem, for they function as decomposers, photosynthetic producers, and is a key component in food chains. By means, microbial metabolism needs to obtain the energy and nutrient, carbon, to live and reproduce. Their decomposition capability is key in reproducing the basis for many food chains. In comparison, without bacteria to recycle carbon from dead organic matter as carbon dioxide, plant photosynthesis would vastly reduce. Cyanobacteria, a blue-green alga, found in freshwater is a phylum of bacteria that obtain its energy through photosynthesis. They are usually unicellular, but they can also grow in colonies. Cyanobacteria is found in freshwater, marine, and terrestrial environments, which distinguishes them from other bacteria because they contain chloroplast. In term of photosynthetic productivity, Cyanobacteria is far more important than rain forests because of the amount of oxygen and nitrogen fixation they can
There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium.
The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes.
See Table 1 and Flow Chart 1 for results of Bacteria # 1 and Table 2 and Flow Chart 2 for results of Bacteria # 2.
There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.
Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).
This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.
There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation
Preliminary studies help identify Genus species of bacteria. Two different preliminary study pathways must be used since two different pathogens were found in the sample. A dilution and a quadrant streak are the ideal methods to separate pure cultures of bacteria. MacConkey Agar and CAN (MAC) is a selective media that is used for the cultivation of gram negative bacteria. (PEA) is a selective media that is used
In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.
Prokaryotes are ubiquitous, successfully adapting to diverse environments as well as developing symbiotic relationships with host organisms (Lengeler, Drews, & Schlegel, 1999). Prokaryotes may have both autotrophic and heterotrophic characteristics. A cyanobacteria is photosynthetic, commonly called blue-green algae, and may produce toxins (Crayton, 1993). Bacteria are most commonly associated in the general
Microscopic organisms known as cyanobacteria are interesting for the following reasons: [SELECT ALL THAT APPLY] Select one or more: A. Oxygen produced by their photosynthesis is thought to be responsible for the "great oxygenation event" about 2.3 billion years ago. B. The methane they produce is a greenhouse gas that could have helped warm the early Earth, helping to resolve the Early-faint-Sun paradox. C. The chloroplasts that carry out photosynthesis in green plants are evolutionary descendents of early cyanobacteria. D. They are known to be the earliest forms of life on Earth.
When oxygen and nitrogen combine in water, it forms nitrate, which is an essential chemical for eukaryotic algae; however, cyanobacteria do not need nitrate for their survival, contrary, they use elemental nitrogen. This way, even after the evolution of true algae, even though in fewer quantity, cyanobacteria remained widespread, and oxygen was accumulated.
This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density
In this lab experiment, students had to create a growth curve for E. coli. The E. coli growth curve would illustrate the progression of the population of E. coli a set time period. In this case, the growth curve depicted the population of E. coli over a 12-hour period. The growth curve for E. coli was created from the absorbance levels, the optical density(OD), recorded from the spectrophotometer.
Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a