Testing A Leaf For The Presence Of Starch

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

Test tube 1 and 2 didn’t show any color change. These two test tubes had lower levels of pH. Therefore, the enzymes could not change the starch to sugar.

enzymes that will be used during this lab to test the ability of amylase to break down starch ,a

5. After testing for glucose, add one drop of the starch indicator solution to both wells to test for the presence of starch. Record your observations.

(2012). Retrieved October 8, 2014, from The uses of Starch: http://www.aaf-eu.org/the-uses-of-starch/ Goodfellow, D., Hocking, S., Rhodes, P., & Musa, I. (2012). BTEC Application of Science: STudent Book. In BTEC Application of Science (1st Edition ed. , pp.

Problem/Purpose: The purpose behind this lab was to create and conduct an experiment to observe cellular photosynthesis. There are many factors that are being observed such as environmental variables, which means how the environment affects the rate of photosynthesis. Photosynthesis is a process used throughout many organisms, the process itself is converting light energy into chemical energy. The process occurs in enzyme-mediated steps that will capture light to convert it into energy rich carbohydrates for other organisms to use. The process can be broken down into the reaction 2H2O + CO2 + light → carbohydrate (CH2O) + O2 + H2O.

This experiment was performed using the procedure from the Photosynthesis in Leaf Disks Lab. First using a #3

For this experiment, we focused on the starch molecules that are contained within the whole-wheat seed.

The purpose of this lab is to understand the process of photosynthesis and how sunlight effect has on it. To prove that in order for photosynthesis to happen light is needed, and to see if temperature has an effect on how fast or how slow photosynthesis happens.

The aim of this experiment was met as the ideal conditions for photosynthesis were found. The hypothesis for this experiment (photosynthesis will occur when Beta vulgaris is exposed to light and CO2) was proved correct after observing the highest percentage of floating disks in the light + CO2 treatment. Overall, it was found that photosynthesis occurs if plants can obtain CO2, water and light (Mudie, K. and Brotherton, J.

Test tube 8 had an initial colour of pale yellow and change to blue-black after the solution reacted with iodine. The result for test tube 8 was positive, proving that it had the presence of starch. Test tube 7 on the other hand, started off with a pale yellow colour and changed to dark brown. The result for test tube 7 was positive, showing that test tube 7 had glycogen content. Test tube 1,2,3,4,5,6 and 10 had negative results, having had no change at all.

this test is quantitative as well. the intensity of the purple color is directly related to the amount of protein present in the sample. Orcinol, when placed in an acidic solution will react with five or six carbon sugars to produce a blue-green color solution. this intensity of the blue-green color reflects the concentration of simple sugars in the sample being tested. I2KI or iodine potassium iodide is a reagent used to test for the presence of plant starches (Polymers of glucose). when mix with this reagent a sample containing starch will develop a blue color. in the absence of starch, the solution will remain a light orange. the intensity of the characteristic blue color is directly related to the amount of starch present in the sample. The last reagent that was used was Niall blue. this reagent is used to detect and measure lipids, specifically fats, oils, and waxes. when heated in the presence of now blue, these lipids turn red. as with the previous tests, the intensity of the red color is a reflection of the concentration of lipids in the

n each test tubes that do not contain the starch solution, add 1 mL of amylase. Then place the test tubes that contains starch solution and the amylase into the corresponding temperature baths. The 0 degree Celsius contains ice to quickly reduce the temperature of the solution inside the test tube and water was added to help the test tube submerge. Allow the test tubes to be the baths for five minutes to

(6.2)Material and Methods in the process or exercise of measuring the starch we were used the following material and how we used them to conduct the experiment. Obtain seven tubes the material to be tested table 6.1 and then add seven to ten drops of iodine to each tube, and then record the color of the tubes contents in table 6.1

Iodine was used to test for the presence of starch, remaining yellow for its negative control and becoming a blue-black colour if a reaction occurred;