Biomolecules Lab

The Benedict test, is a test to determine whether a solution has reducing or non-reducing sugars. If the Benedict's test is negative, that means there is not reducing sugars. Next, the Lugol's test is to determine if there is starch in a substance. If the solution is yellow or orange after a Lugol's test, that means it is negative. If the results of a Lugol's test is purple or black, then it is

Using Benedict's testing on our food items, we tested for simple sugars. Six clean test tubes were labeled individually with each testing food item plus one negative control test tube. Solutions of each food item in the quantity of one full dropperful was added to each labeled test tube and one full dropperful of water was added to the control tube.

The objective of the Reducing Sugar Test was to test if the substance has a reducing sugar in it by adding Benedict’s solution and heating it, there would be a color change if a reducing sugar is present, or it will remain blue (no reducing sugar). The objective of the Starch test was to test for starch in substances by using Iodine. The iodine will cause a substance to turn to a dark blue color if it is positive for starch. The objective for the Grease Spot Test was to test if the substance had a lipid. A positive reaction would make a translucent grease mark on a brown paper bag. The objective for the Dye test was to test for lipids. When mixed with water and the tested

1. Carbohydrates: Are Molecule composed of carbon, hydrogen, and oxygen, and they include sugars and starches.

We then recorded the initial color. We placed each tube in boiling water for one minute and recorded the color results and gave our conclusion. To test for starch using Lugol’s solution, we reused the test tubes and added a squirt of the solution. We recorded the final color and then our conclusion for each content. To test for lipids using paper towels, we placed a drop of solution and we let it stand for one minute. We then recorded our observation, if it was dry or not dry and wrote our conclusion for each sample. To test for proteins using Biuret’s reagent, we added a squirt of stock solution plus a few drops of Biuret’s solution. We wrote the initial color. We then shook the solution and waited for two minutes before recording the results. After the two minutes, we wrote the final color and conclusion for each content. For the unknowns, we wrote the odor and appearance of each content and then tested the benedict’s, starch, lipid, and protein test and wrote our conclusion.

The purpose of this experiment was to see the last place where the murder victim went to. The objective of this is to see if his contents contain the very following lipids, proteins, carbohydrates-glucose, and carbohydrates-starch. When testing for lipids it tested negative. The paper bag was not translucent to show that they were any signs of lipids. When testing for carbohydrates-glucose. The heated Benedict compound was still blue meaning there was no signs of there being glucose. This might have happened because something went wrong while testing like for example, all of the foods suppose to have lipids when the group tested negative for lipids. There must have been a low amount of polysaccharides and fatty acids that they could not be

Introduction: In this lab, we studied biomolecules. Biomolecules are molecules that are existent in all living organisms. The four biomolecules, we are learning in class are proteins, carbohydrates, lipids, and nucleic acid. The three biomolecules being addressed in the lab are proteins, carbohydrates, and lipids.

One milliliter of liquid was pipetted into three test tubes each making a total of nine test tubes. Biurets reagent, Lugol’s iodide, and Benedict’s solution were the reagents used to help identify the unknowns. One milliliter of each reagent was added to one protein test tube, one starch test tube, and one glucose test tube. We placed the test tubes with Benedict’s solution in a hot water bath for 5 minutes and recorded what happened. The three supplements acquired were tested using the same techniques used above and were compared to each other. The objective in this experiment was to determine what was in each

2-1: Which tubes showed that starch or cellulose was still present? Tubes ___4__, ____5__, __6___

We received results that made sense and visible errors were made. However, there was room for error; the amount of food put in each test tube was not exact, nor was the amount of solution added to the test tubes. Also, we did not time how long we heated up Benedict’s Solution for, so each test tube had a different amount of time in the water, which could have affected the results. The solution were supposed to turn the foods different colors based on what molecules they contained, but since some of the colors weren’t exact the shades as shown on the indicator sheet, we had to

For this experiment, we have to prepare our phosphorylase which extracted from a potato. We prepared by weighed about 250 grams of peeled potato and cut it into cubes. The extracts then blended with 100mL of 0.1M NaF. After filtered the contents into a clean 250mL centrifuge bottle, we centrifuged it for 3 minutes. Then, separated the supernatant into a centrifuge bottle, which is our phosphorylase preparation. The enzyme assay used in this experiment today is the iodine test. As the iodine reacts with starch, it will form a brown, blue or black precipitate due to the iodine ions forcing into a linear arrangement. The endpoint of the enzyme reaction indicates the presence of starch by using the iodine test to determine. The faster the endpoint is reached, the less active the phosphorylase is.

Measurements of Atoms and Molecules in Solids, Liquids and Gasses Lab Report Introduction: Four experiments were conducted in this lab: measurement of atomic size in metal lead and zinc, finding the packing coefficient using lead shot, measurement of molecule size in water, and determination of an empty space percentage in gaseous CO2. We performed these experiments with simple measurement and calculation techniques to estimate the size of atoms and molecules of solids and liquid, and the empty space percentages of solids, liquids and gases. Procedure: Experiment 1: Measurement of atomic size in metal lead and zinc

When iodine was added to all four solutions, only one reacted and changed color. Only the polysaccharide solution, test tube #3, reacted which was expected. When performing the Benedict’s test, a reaction occurred almost immediately after the test tubes were put into the water bath. Both test tube #1, the monosaccharide solution, and test tube #4, the unknown solution, changed color from blue to a yellow-orange when heated. Since the unknown solution reacted during the Benedict’s Test, used to identify monosaccharides, and not in the Iodine Test, used to identify polysaccharides, that means the unknown solution #10 is a monosaccharide

The Abstract: The analysis of the molecules of life through the functional groups of the different varieties of macromolecules, to demonstrate the strong correlation between experimental and expected results through the colour from the chemical reactions. It is to show evident support on where to find the strong colour results through the tests of Benedict’s, Sudan III, Iodine and Biuret. Aim: The Molecules of Life’s aim is to use four different chemical tests in order to identify carbohydrates, fats, proteins and oils in various substances through the tests of Benedict’s, Iodine, Sudan III and Biuret.

During the Benedict's test, the contents of tube B did not change, indicating the absence of sugar in that particular substance. However, the contents of tube A did change orange indicating the presence of sugar in that substance. During the Lugol's test, the content of tube A did not change dark purple indicating the absence of starch in that substance However, the content of tube B changed to dark purple