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Blunt Dissection

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Sixteen adult local bucks between (18-24) months of age and (25¬±3.8) kg body weight. Animals were allocated randomly and equally into two treatment groups the first group was castrated with injected (5) ml of 3% formalin. The second group was castrated by double bilateral ligations of the vascular part of the spermatic cord. All bucks were clinically examined and confirmed to be free from any obvious abnormalities of the palpable reproductive organs. The animals were restrained in lateral recumbency with the help of xylazine hydrochloride 2% at dose rate of (0.05 mg/kg) injected IM. Then local anesthetic, lidocaine hydrochloride 2% at a dose rate of (0.6 mg/kg) via S/C., infiltrated at the sites according to the method described by (Thurmon…show more content…
The same technique was repeated on the contra-lateral testis. (Fig.1). In double bilateral ligations of the vascular part of the spermatic cord group, buck was carefully draped with sterile drapes to maintain an aseptic surgical field. A straight incision approximately 1.0 to 1.5 cm. long is first made through the skin covering the spermatic cord. Blunt dissection is made to separate the spermatic cord from the surrounding structures. Then the cord is exteriorized through the skin incision by curved artery forceps and tunica vaginitis is opened to expose the testicular vessels. The testicular artery and veins were identified and double transfixation ligatures are placed around the testicular vessels at two points about (2 cm) apart with non-absorbable suture material such as silk (size 1) (Fig.2). The tunica vaginitis is restitched with fine absorbable suture material (cat gut size 3/0). Finally the skin is closed by silk with 3-4 stitches of simple interrupted pattern. The same technique is repeated on another cord. Testicular biopsies were harvested and fixed in 10% neutral buffered formalin and passed routinely to prepare histopathological sections and were stained with
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