Calorimetry Lab Report

1.8 ml is saved to freeze for restriction digests. Then add 200 μl of resuspension solution inacti-

The mixture was heated at 120°C using an aluminum block and was stirred gently. After all of the solid dissolved, it was heated for 20 additional minutes to ensure the reaction was complete.

Fill a test tube about 1/3 full with cold tap water for use in step 34.

2. Used a test tube clamp to held the test tube and gently heated the tube in a laboratory burner flame for about 30 seconds.

Fill granulated cylinder with water by submerging and make sure no bubbles at the top.

The aqueous extracts were combined in an Erlenmeyer flask and cooled down in an ice bath to 10C.

This was done for 15 minutes until there were no visible vapors coming from the reaction mixture. The reaction mixture was then poured into a 50-mL beaker containing 5g of ice. The reaction flask was rinsed three times with 2 mL of dichloromethane. The rinsing was also added to the ice mixture.

Boil up the mixture on medium warmth until the water is decreased by a third and after that let it cool for some time.

Apply heat to a hot water vapor, 5-7 minutes, constantly messing, until the mixture is homogenous.

Reflux the mixture for 30 minutes, adjusting the flame to ensure smooth boiling. Ensuring that the mixture is refluxed for 30 minutes guarantees the reaction completes so that the vapours continuously condense for re boiling. 6. Put 20cm3 of distilled water into a separating funnel. Pour the cooled mixture into the separating funnel.

Hold the melting point tube open side down and carefully pick up/push crystals into the tube. Turn the tube and tap the closed end gently on the bench to “knock” the crystals to the bottom of the tube. The bottom of the tube should be about 2 mm full.

Discard the solution in the appropriate container as directed to you by your lab instructor.

To tube 2 and tube 3 a boiling chip is added. The two tubes are boiled to remove any residual ether. Next, the tubes are cooled to room temperature and placed into an ice bath to allow for crystallization. The solution is then removed from the solid in each tube and discarded. To tube 2 and 3 ~0.5 ml of H2O is added for recrystallization, the tubes

As moisture starts to condense in the upper part of the test tube, carefully heat the entire length of the tube. Continue doing this for 2-3 minutes until all of the moisture is driven from the tube. Repeat steps 3 and 4 for the other two tubes.

Use either bowl, sink or tub with warm to hot water. Adjust the temperature to your preference, but it needs to be warm for this process.