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Carolin Bags Research Paper

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As human kind has evolved and further explored the scientific world, the use of animal models has allowed an understanding of biology never before seen throughout time. The most common animal model, the mouse, has been studied so vigorously, including transgenic and knockout strains,1 that a new strain of ‘super-mouse’ is being designed with excessively fast reproductive time, large litters, and more factors that in turn, essentially make it the perfect animal model.1 However, research is drifting from the use of this small mammalian creature towards other, more complex, species; one of which being the Anolis carolinensis, otherwise known as the Green Anole Lizard. A. carolinensis has become the frontrunner of genomic sequencing for the Class …show more content…

carolinensis. Therefore, despite being such a small lizard, A. carolinensis has made an incredible impact in the scientific realm. Although genomic sequencing has advanced ten-fold throughout the 21st Century, there has been little focus on non-avian reptiles3 until A. carolinensis became the first non-avian reptile to have its entire genome sequenced and published in 2011.5,6 Native to North America,5 A. carolinensis are an incredibly abundant species of amniotic vertebrates4 and have been …show more content…

carolinensis is such a popular animal model that many research articles have been published around the experimentation. Elizabeth Hutchins (2014) focused on the regenerative abilities found in the lizard’s tail and the cells that worked specifically during the process. The study used a variety of experimental methods to collect data including; applying pressure to their tails until they were released, this site of release was sampled and analysed with RNA sequencing, immunohistochemistry, etc. to isolate different cells, and therefore regenerative processes.4 Ultimately, the regenerative tail of A. carolinensis showed a multitude of genes being activated that are specific to wound healing and musculoskeletal development pathways.4 Skeletal and cartilage growth occurred along the entire length of the tail and there was no localised bundle of proliferative cells, alongside a highly vascular distal end;4 all different mechanisms to the localised, distally avascular mechanism found in anamniotic vertebrates.4 This stem and progenitor cell based process is therefore quite different to the previously studied ‘blastema-based model’ of regeneration in the commonly studied anamniotes: zebrafish and salamanders, providing insight into application to further medical regenerative possibilities in humans.4 Using A. carolinensis provided a prominent advantage to the study due to the previously highly developed genetic knowledge, ease of replenishing populations, and habitual

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