Cell Tolerance Test And Growth Temperature Test Essay

The Unknown Bacteria 36/Bacteria # 2 on a TSA plate was examined by the naked eye and under a dissecting microscope. Bacteria # 2 was approximately 3 - 4 mm in diameter. They were circular in form with an entire margin and a flat elevation. The colonies were rough (granular), translucent, and white brownish color with black granules. The Gram stain resulted in a Gram negative rod. After the Gram stain was completed, the bacteria were streaked on an Eosin -Methylene Blue Agar plate and an Enterotube II was inoculated.

This experiment illustrates the importance of handwashing and proves that hand washing is worth it. Since our hands are constantly coming into contact with ourselves and others, touching surfaces, grabbing objects, being sneezed into, etc., keeping our hands clean is one of the most effective, yet simple way we can take to avoid getting sick and spreading germs to others. Many diseases and conditions are spread by not washing hands with soap and clean, running warm water. “The human skin is a host to anywhere between 10,000-10,000,000 bacteria per square centimeter and since health care providers come into contact with pathogenic bacteria by being engaged in patient care, hand washing can reduce the risk of spreading diseases (page 3).” The objective of the experiment is to test the effectiveness of hand washing and demonstrate normal flora. This report presents the procedures and materials for the experiment, the experiment's results, and an analysis of those results.

For many years the identification of microorganisms has been important in the world of medicine. It is essential or correct disease diagnosis in patients and for proper treatment. Knowing the correct identity and characteristics of microorganism is crucial when disease outbreaks occur in populations, also knowing how humans can benefit from microorganisms is important; many can be used in making certain foods or antibiotics.

According to the “Microbiology Laboratory Manual,” lab 27 focuses on the family Enterobacteriaceae, this is one of the largest families of bacteria (Wong et al., n.d.). Also all of the members are gram-negative, anaerobe straight rods and can commonly be located in mammal’s intestines (Wong et al., n.d.). All the members Enterobacter aerogenes throughout this report will be referred to as, E. aerogenes. There were several examples where E. aerogenes was tested or used, the first example is a One-pot synthesis, the next example is where it is used for dark fermentative biohydrogen production, and the last example is where it is analyzed in groundwater in North Central Nigeria.

The main idea of this experiment was to correctly identify the unknown bacteria, #3. Identification of unknown bacteria yields multiple benefits in many different areas in the research of microorganisms. In this experiment I performed many different test dealing with things such as the presence of enzymes, fermentation abilities and different chemical reactions. Observations made from the tests were then compared to a gram negative unknown chart in order to identify the bacteria. Based off of my results and the chart, I concluded the bacteria #3 was the bacteria Escherichia coli. E. coli is most commonly found in the intestines of warm blooded organisms. Most E. coli strands are non pathogenic however, there are strands

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

The bases of this experiment was to discover the identify of the unknown from three possible specimens: Klebsiella pneumonia, Escherichia coli, and Enterobacter aerogenes. Utilizaing the T streak technique, the bacteria was isolated into pure colonies for further study. The Gram Stain method was used to identity the morhphology of the bacteria such as the shape and whether the bacteria was Gram positive or Gram negative. Biochemical test were also used to help identify the unknown bacteria. The biochemical test used was the Triple Sugar Iron Agar, Sulfur Indole Motility test, Methyl Red test, Voges-Proskauer test, Citrate test, Urease test, and the Gelatin test. After observing the morphology of the bacteria using the Gram Stain method and utilizing all the possible biochemical test, the bacteria was identified to be Enterobacter aerogenes.

The priority nursing diagnosis of hospital acquired infection is risk for any kind of infection. One of the main goals for each patient in the hospital is the patient will remain free of infection as evidence by absence of heat, pain, redness, or swelling in any area of the patient’s body during each nurse’s shift. (care plan book). Frequently hand washing is the best intervention for preventing infection. Hand washing reduces the risk of transmission of pathogens by inhibiting the growth of or killing the microorganisms. (cb)Proper sterile technique during urinary

pneumoniae I believe I have a subspeciesof K. pneumoniae perhaps K. pheumoniae ozaenae or K. pneumoniae rhinoscleromatis. Indole, MR-VP and Citrate will vary among different subspecies according to bergey's manual of determinative bacteriology. The reason why I decided on K. pneumoniae over E. aerogenes is because of the gelatin and motile test. According to bergey's manual, Enterobacter liquefies gelatin very slowly and in addition E. aerogenes will test positive for motility. To further differentiate K. pneumoniae from Enterobacter and narrow down the exact species of K. pneumoniae the urea hydrolysis assay could have been the determining factor. Subspecies ozaenae and rhinosclermatis will result in a negative reaction for VP but bergey’s Manual indicates a positive reaction for

Enterobacter aerogenes is a Gram-negative, rod shaped bacteria, from the Genus Enterobacter. Several experiments were performed to properly indicate the bacteria, such as the citrate utilization test, motility test, and glucose fermentation test. All of which tested positive. The purpose of these multiple tests was to become familiar with the process of testing, the different types of tests, and how to identify positive and negative results to properly conclude and identify the correct bacteria Genus and species.

The bacteria that was contained within Unknown tube #12 is believed to be Pseudomonas aeruginosa, Figure 1. The bacteria tested to be Gram Stain negative, producing a pink, red color retained from the staining process. When the species of bacteria was plated on nutrient media, the cells produced an irregular and spreading configuration as shown in Figure 2. This same plating test provided the margins and elevation, lobate and hilly, respectively. The specimen was stabbed in a Fluid Thioglycollate Medium (FTM) tube using an inoculated loop of the bacteria. The results of this experimentation indicate the type of oxygen requirement of the bacteria. The test found the bacteria to be aerobic as colonies of the bacteria began to form along the top of the FTM tube (Manual 2017).

This gram negative bacteria, Escherichia coli, can survive for 1.5 hours to 16 months on dry inanimate surfaces.( Public Health Agency of Canada,

Enterobacter aerogenes is a species of rod shaped gram negative bacterium in the family of enterobacteriaceae. It is a facultative anaerobe, meaning that it can grow in either the presence of oxygen or in the absence of oxygen. It also uses fermentation as the means of gathering energy that protect it from reative oxygen species, and allowing it to live in oxygenated environments. Enterobacter aerogenes is a nosocomial and pathogenic bacterium that cause opportunistic infections. The majority are sensitive to most antibiotics designed for this bacteria class, but it can be complicated due to their resistance mechanism such as lactamas, which quickly become resistant to standard antibiotics during the treatment. Enterobacter aerogenes is found in human gastrointestinal tract, and it does not generally cause disease in healthy individuals. It has been found to live in

The two experiments performed in this lab to determine the factors that affect cell growth. Part 1 was used to determine the effects of differential media and other conditions necessary for cell growth. The second part studies how cells respond in suspension and the formation of Multicell Tumor Spheroids (MTS). The cells were initially calculated using the Neubauer method, the cells were then separated in certain concentrations to be transferred to variously prepared plates and wells. It was expected that a higher amount of growth would occur in IDMEM media compared to MEM media. This was due to the richer nutrients of the IDMEM media. While the amino acids, glucose, and vitamins that are not found in MEM media restrict the growth of cells. Similarly, the IDMEM media with 10% serum was also expected to have more growth than the MEM with 10% serum due to its richer nutrients. Therefore, the IDMEM with 10% serum should have the most abundant growth, followed by IDMEM, MEM with 10% serum and finally MEM with the least amount of growth. The 10% serum also affects the cells, serum contains growth factors and adhesion factors that are responsible for cell proliferation and cell attachments. The plates that consisted of agarose should also show

This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.