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Cellular Respiration Lab Report

Decent Essays

Title: Production of Carbon Dioxide, During Aerobic Respiration

Author: Olivia Huertas

Abstract: In this procedure, phenolphthalein will be used to detect changes in pH resulting from the production of CO2 during cellular respiration. Because phenolphthalein is red in basic solutions and colorless in acidic solutions, you can monitor cellular respiration by measuring acid production as change in pH. CO2 produced during cellular respiration can combine with water to form carbonic acid. By measuring the volume of NaOH used to neutralize the carbonic acid produced by the CO2, and thereby calculate a proximate measure of respiration.

Hypothesis: If the fish and elodea both produce CO2 during cellular respiration, then the Elodea will produce …show more content…

The solution has been dechlorinated and adjusted to be slightly acidic. Place 75 mL of the solution in each of three labeled beakers. Obtain an animal organism, small fish, and a plant organism, Elodea. One beaker will be the control and will not have anything in it. Place exactly 25 mL of water in a 50-mL graduated cylinder. Place each organism in a cylinder and note the increase in volume above the original 25mL. The increase equals the volume of the organism. After taking measurement, cover each beaker with the plastic film. Place the beaker containing the Elodea in the dark by covering it with aluminum foil. Allow organisms to respire for 15 min. Gently remove the organisms from the beakers and return them to their original culture bowls. Then add four drops of phenolphthalein to the contents of each beaker. The solutions should remain clear because the solutions are acidic. Using a dropper bottle, dispense NaOH into the contents of the beaker drop by drop. Thoroughly mix the contents of the beaker after adding each drop. Continue adding drops until you first notice that the solution turns pink. Repeat for each beaker with at the living organism until the solution is the same shade of pink as the

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