Cellular Respiration and Fermentation: Experimenting With CO2 and Redox Reactions
Julius Engel; Section 8
Abstract
In this experiment, the subjects of study were fermentation, mitochondrial respiration, and redox reactions. In the first experiment, yeast was grown in various carbohydrate solutions at various temperatures. In the second experiment, succinate was added to various samples of a mitchondrial suspension, DPIP, and a buffer. Then after two blanks were used, the samples were placed into the spectrophotometer for transmittance testing.
Introduction
Cellular respiration is a group of reactions that occur when a cell turns the energy from food and nutrient sources into ATP, releasing the rest of the
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The goal of this experiment is to successfully stage and observe redox reactions in mitochondrial respiration and fermentation, to better understand these processes. As for hypothesizing, prior to the lab I thought that glucose at 37 degrees would be the greatest yield of CO2 and that as time went on, the transmittance readings of the reduced DPIP would decrease. Neither was entirely correct. Sucrose at 37 degrees seemed to yield the most CO2 and the results for the transmittance readings of the reduced DPIP varied by sample.
Methods and Materials
Fermentation
In part one of the experiment, saturated starch and 4 degrees celsius were the assigned food source and temperature respectively. .5g of yeast was added to 15 mL of the solution, which was immediately transferred into a fermentation tube and stored in the refrigerator with the rest of the 4 degrees samples. Fermentation was then allowed to occur for 40 minutes, with the amount of CO2 produced recorded every 5 minutes. CO2 was measured by reading the level as compared to the markings on the fermentation tube.
Mitochondrial respiration/Citric acid cycle In part two of the experiment, the spectrophotometer was turned on and set to read the % transmittance of 600 nm wavelength light. 6 cuvettes were then obtained and labeled B-1 and B-2 (to
Fermentation is a metabolic pathway that produce ATP molecules under anaerobic conditions (only undergoes glycolysis), NAD+ is used directly in glycolysis to form ATP molecules, which is not as efficient as cellular respiration because only 2ATP molecules are formed during the glycolysis. One type of fermentation is alcohol fermentation, it produces pyruvate molecules made by glycolysis and the yeast will break it down to give off carbon dioxide, the reactant is glucose and the byproducts are ethanol and carbon dioxide. In this lab, the purpose is to measure whether the changes of
There are two types of cellular respiration, aerobic and anaerobic. Aerobic respiration occurs when there is oxygen present and in the mitochondria (in eukaryotic cells) and the cytoplasm (in prokaryotic cells). Aerobic respiration requires oxygen; it proceeds through the Krebs cycle. The Krebs cycle is a cycle of producing carbon dioxide and water as waste products, and converting ADP to thirty-four ATPs. Anaerobic respiration is known as a process called fermentation. It occurs in the cytoplasm and molecules do not enter the mitochondria for further breakdown. This process helps to produce alcohol in yeast and plants, and lactate in animals. Only two ATPs are produced through this process. In yeast fermentation is used to make beer, wine, and whiskey.
Respiration is a chemical process by which organic compounds release energy. There are two types of respiration reactions that cells use to provide themselves with energy: aerobic and anaerobic (fermentation). (Chemistry for Biologists: Respiration. 2015) Both processes are similar within the initial steps of the reaction- beginning with glycolysis. However, in fermentation instead of the pyruvic acid being converted to acetyl coenzyme A, it’s converted into both ethanol and carbon dioxide in yeast and some plants and lactic acid in animal cells. Another distinct difference between the two processes is that anaerobic respiration uses oxygen
Cellular respiration is the series of metabolic process by which living cells produce energy through the oxidation of organic substances. Cellular respiration takes place in the mitochondria. Fermentation is the process by which complex organic compounds such as glucose, are broken down by the action of enzymes into simpler compounds without the use of oxygen. The significance of these pathways for organisms is to allow for an organism to be able to generate ATP. Some organism that undergo cellular respiration are bacteria and fungi. Some organism that undergo fermentation are yeast and muscle cells. In cellular respiration, glucose is oxidized and releases energy. In cellular respiration, glucose produces ATP and 3-carbon molecules of pyruvate. The pyruvate is then further broken down in the mitochondria where it becomes oxidized and releases CO2 (Upadhyaya 2014). In the fermentation process oxygen does not play a part. This process converts glucose into pyruvate and produces ATP. From there pyruvate breaks down into CO2 and acetaldehyde (Upadhyaya 2014) Monosaccharides are known as simple sugars and their main function is being the source of energy for organisms. Disaccharides are two monosaccharides joined by a covalent bond and their primary function is to provide food to monosaccharides. Some disaccharides
This lab investigates the effects of Sucrose concentration on cell respiration in yeast. Yeast produces ethyl alcohol and CO2 as a byproduct of anaerobic cellular respiration, so we measured the rate of cellular respiration by the amount of CO2
The CO2 production was measured with a CO2 meter (+/- 1ppm), in order to determine the rate of anaerobic cell respiration in yeast.
The procedure for this experiment was to first obtain four balloons and blow them up in order to stretch them. Then obtain and fill the four large test tubes each with thirty milliliters of warm forty degrees Celsius water and two grams of dry yeast which was weighed on a scale and scooped out by a spatula. After five milliliters of water, ten percent glucose, fructose or sucrose went into one of the four test tubes. Then parafilm was placed on top of each of the test tubes to seal them and they were swirled activating the yeast through rehydration. After swirling the film was removed and the balloons were tightly placed on the test tubes. Then finally observed the tubes build up of CO2 all the while swirling gently every fifteen minutes, recording observations.
Thirdly, chemical reactions keep us alive in the process of cellular respiration. Cellular respiration is the process by which the energy that we consume from our food is converted into energy that can be used by our cells. Cellular respiration is composed of a series of chemical reactions, including redox reactions. What are redox reactions? Simply put, they are reactions in which one molecule gives an electron to another molecule.
lysis of Cellular Respiration Under Various Conditions Introduction: These experiments provided insight into the process of cellular respiration in aerobic and anaerobic conditions with the variable factors such as the presence of succinate, temperature, and variable carbohydrates. Cellular respiration is the set of metabolic reactions that transform glucose and other reactants into useable energy (ATP) and waste products (Saylor, 1). In the first part of the experiment, the impact of succinate toward aerobic respiration is observed. By utilizing the suspension of mitochondria, the conversion of succinate to fumarate can be examined in the citric acid cycle. Using spectrophotometer, the transmittance of the color change is facilitated by
This experiment focus on fermentations. Fermentation is a partial degradation of sugars that occurs without the use of oxygen gas. Cells that have no oxygen in their environment can use fermentation to obtain energy from their foods. The amount of ATP made in aerobic (oxygen present) conditions. Only 2 ATP molecules are made from one glucose molecules during anaerobic conditions (glycolysis + fermentation). Carbon dioxide and ethanol during alcohol fermentation, or lactic acid during lactic acid fermentation, are waste products of the fermentations reactions. Fermentation is vital for many organisms, such as yeasts, certain molds, and bacteria, because it allows them to obtain energy required to carry on life processes. Yeast, a single-celled
To prepare experiment 1, labels were put on four separate 50 mL plastic tubes. They were each labeled, “Negative control A”, “Positive control B”, “Positive control”, and “Experimental”. Fermentation tubes were also labeled in the same way as the vials. Next, glucose in the amount of either 2% or 6% and water was extracted from beakers using a pipette pump. The water and glucose were then mixed in the plastic tubes before adding the yeast from a separate beaker, also extracted by a pipette pump. It was important to make sure that the yeast is added last, as the reaction will happen as soon as yeast and sugar come into contact. After the solutions were mixed together, they were put into their previously labeled fermentation tubes. After the
Cellular respiration is the group metabolic reactions that happen in the cell of living organism that creates adenosine triphosphate, ATP, from biochemical energy. The formula for cellular respiration is C6H12O6 +6O26CO2+6H2O+ATP. This formula means glucose and oxygen are turned into water,carbon dioxide and adenosine triphosphate (ATP) energy through chemical reactions. Cellular respiration occurs in all cells which allows them to grow. Raphanus raphanistrum subsp. Sativus seed, also known as radish seed, undergo cellular respiration because they are not yet able to perform photosynthesis, which is how plants create their energy. Hymenoptera formicidae,commonly known as ants, undergo cellular respiration to produce the energy they need to live.
Glucose and oxygen enter the mitochondrion for the processes of cellular respiration. Through a series of chemical reactions ATP is produced. Then carbon dioxide and water are created.
We filled three beakers each with 120 ml of 10% sucrose. In the first beaker we added no ethanol to the 120 ml of 10% sucrose for a 0% solution, we then added 6 ml of 99% ethanol for a 5% solution in the second beaker, and added 12 ml of 99% ethanol for a 10% solution in the third beaker. We then took 40 ml of each solution in separate beakers and stirred in .4 grams of yeast. After allowing the yeast to proof, we poured each solution into their own fermentation tubes and then placed them in a 37℃ water bath measuring (mm) how much carbon dioxide was produced every five minutes for thirty minutes. This was repeated two more times with each solution.
Spectrophotometer was turned on and allowed to warm up while samples were being produced. The mitochondrial solution was kept cold on ice to prevent spontaneous enzymatic reactions.