Ceratopteris Fern Growth and Analysis: Observing Germination and Effects of Ammonium Nitrate Treatment
Observing the effects of ammonium nitrate has on the germination days of C-Fern gametophytes by constructing an experiment with two spore-sown petri dishes, one control and one treatment – a normal nutrient-rich agar petri dish and an ammonium nitrate-containing petri dish respectively. The two petri dishes were each inoculated with three drops of spore suspension by a pipet, then spores were spread by a sterilized-bent paper clip to allow even distribution of spores. Gametophyte germination occurred approximately two weeks after inoculation for the control, and gametophyte germination occurred…show more content… Spores were sown in the week of September 10th and no observations were made. First observation was made in the week of September 17th, Friday 3:08 pm, the control displayed some growth with flagella-like hair, however, no germination was observed. The treatment did not display any signs of growth, only air-like bubbles were observed. Second observation was made in the week of September 24th, Friday 3:10 pm, the control displayed germination, gametophytes were visible under the microscope, however, hermaphrodites were not significantly observed. The treatment still does not display signs of germination, only small green spores were observed under the microscope. Last observation was made in the week of October 1st, Friday 3:05 pm, the control displayed significant signs of growth, it appeared that fertilization took place and an embryo was in development. The treatment only displayed minimal germination, however, some spores appeared to be infested with fungus.