Introduction Carbohydrates are defined by the following classifications: polyalcohols with aldehyde (known as aldose) or ketone (known as a ketose) groups, reducing molecules and water soluble. Additionally, carbohydrates can be grouped in several ways, as either monosaccharides, disaccharides or polysaccharides. Monosaccharides have one carbohydrate unit. Disaccharides have two carbohydrate units. Polysaccharides have many carbohydrate units. An example for each includes: glucose (monosaccharides), sucrose (disaccharides) and starch (polysaccharides). The initial purpose of the identification of unknown carbohydrates experiment was to identify unknown carbohydrates based on chemical and physical properties and understand that similarities and differences exist dealing with known carbohydrates.
Results
The following unknowns were tested: Unknown Carbohydrate 5 (Carb. 5) and Unknown Carbohydrate 7 (Carb. 7).
A. Fermentation Test:
Carb. 5 was positive for fermentation while Carb. 7 was negative for fermentation.
B. Benedict’s Test for Reducing Sugars:
Both Carb. 5 and Carb. 7 tested positive for the Benedict’s test. Based on the reaction timing, Carb. 5 reacted faster than Carb. 7 with the Benedict’s solution. Carb. 5 took a total of 3 minutes and 11 seconds to go through the color change that helps indicate whether it is a reducing sugar (blue to green to yellow to brick red), while Carb. 7 took 4 minutes and 33 seconds.
C. The Seliwanoff Test for Ketoses:
Both Carb.
1. Fill in the table below with the results from the monosaccharide test experiment, and your conclusions based on those results.
The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test
A cell, the building block of all living organisms, is composed of four fundamental biomolecules: proteins, carbohydrates, sugars and lipids. Proteins provide a vast amount of functions cells such as they serve as enzymes, provide structural support to cells, and act as antibodies. Reagents are used to spark a chemical reaction. The reagent used to detect protein traces in a substance is Biuret’s. Biuret’s will turn purple if proteins are present and blue if they are none. Biuret’s copper particles, have a charge of +2, are diminished to a charge of +1 when peptide bonds, which are in proteins, are present, creating the color change. Polysaccharides, which are carbohydrates, are most notably known to provide energy to the body, but they also help in breaking down fatty acids. Iodine is the reagent used to determine whether a substance has starch in it. The iodine/starch complex has energy levels that are only for retaining unmistakable light, giving the complex its extraordinarily dark black-blue shade. If there is no starch found, iodine will remain its natural yellowish-brownish color, but if starch is present, iodine will turn blue-black. Monosaccharides, which are sugars, like polysaccharides, provide the body with energy. To detect monosaccharides, the reagent, Benedict’s, is used. Benedict’s reagent is added to a test tube, then it is placed in
(Driskell 29) These simple carbohydrates are easily broken down, converted into glucose for the body and easily flow into the blood stream. The downside to this form of carbohydrates is that they are most common in the form of refined sugars or refined flours which retain very few vitamins after being processed. (“HealthStyle Fitness”) As the body uses this form of glucose a cycle begins which involves fluctuations of high and low blood sugar. This cycle works when blood sugar rises after the consumption of simple carbs and then as the blood sugar begins to drop the body demands more simple carbs to bring blood sugar levels back to up. The cycle that is formed essentially keeps the body from burning body fat because there is a continuous demand and consumption of simple carbohydrates. The second form of carbohydrates is that of complex carbohydrates which consist of polysaccharides, three or more monosaccharides. These complex carbs are found in the form of starch, fiber and glycogen (animal starch). Those complex carbohydrates consumed in the form of starch are found in plant foods like corn, wheat, potatoes, cereals, pastas, and beans. When they are broken down these carbs allow for the slow production and release of glucose into the blood stream and allowing for a consistent blood sugar level. (Guthrie 27) Dietary fiber is another form of complex carbs which are present in plant foods because fiber is
2. Four unknown samples were included in the lab kit: flax seed meal, potato starch, egg whites, and dried milk. Using the results of the biochemical testing, determine which number corresponds to the correct unknown. (8 points)
7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.
Carbohydrates are composed of carbon, hydrogen, and oxygen. Carbohydrates can come from fruits, vegetables, and grains. There are two different kinds of carbohydrates. The first being simple carbohydrates, which are sugars. Glucose, fructose, and galactose fall under the sugar category. Then you have your complex carbohydrates, which are starch in grains, glycogen stored in our muscles, and fiber. When it comes to energy within our body glucose is a major source of energy. When our body does not get enough carbohydrates to supply enough glucose, our body will make glucose from proteins. I applied this to my life by making sure I eat fruits, vegetables, and grains to get my carbohydrates every single
Carbohydrates are macromolecules which and contains carbon, hydrogen and oxygen (C,H,O). There are also two types of sugars, sweet soluble and starches which are non-soluble. Sugars can also be single or simple sugars.Carbohydrates can be found in a wide variety of foods such as pasta, wheat and grains, rice, potatoes, fruits and sugars. Carbohydrates are broken down into
14.While working a part-time at a lab, the student is asked to grab a jar that contains carbohydrates. Two jars are on the counter, each labeled with their chemical formula. One is labeled C5H10O5, and the other is C3H9O3. (6 points)
We tested honey, egg, saltine, and hamburger. We tested sugars using Benedicts solution and boiling water. We tested for starch using Lugol’s solution. We tested lipids using paper towls. Lastly, we tested proteins using Biuret’s reagent solution.
The purpose of this experiment was to see the last place where the murder victim went to. The objective of this is to see if his contents contain the very following lipids, proteins, carbohydrates-glucose, and carbohydrates-starch. When testing for lipids it tested negative. The paper bag was not translucent to show that they were any signs of lipids. When testing for carbohydrates-glucose. The heated Benedict compound was still blue meaning there was no signs of there being glucose. This might have happened because something went wrong while testing like for example, all of the foods suppose to have lipids when the group tested negative for lipids. There must have been a low amount of polysaccharides and fatty acids that they could not be
This is because glucose is the simplest type of carbohydrate (monosaccharide). However sucrose is a
Carbohydrates are the product that made up from carbon, hydrogen and oxygen. Carbohydrates are form by the combination of carbon dioxide and water molecules. The carbohydrates contain two specific functional group in it which is the hydroxyl groups and carbonyl groups.A reducing sugar is a type of sugar with is an aldehyde group.This means that sugar can act as a reducing agent.The procces of reducing sugar is isomerisation,example of reducing sugar islactose,maltose,glucose and fructose.All monosaccharides are capable of reducing other chemicals such as copper (II) sulphate to copper oxide.Beside that disaccharides such as maltose and lactose are reducing sugar,however sucrose is non reducing
Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.
The term ‘’Carbohydrates’’ is defined as the carbon compounds which contains hydroxyl groups in large quantities (King, 2013). Carbohydrates are those organic compounds which have the empirical formula CnH2nOn, or (CH2O)n (Fromm 1997). Carbohydrates are one of the four types of organic compounds in living cells that are produces during photosynthesis and are the important source of energy in both plants and animals (Ahmed 2014). They are built from monosaccharides. These monosacchradies are the small molecules that consists of three to nine carbon atoms and differ in size and in the stereochemical configuration at one or more carbon centers. The simplest carbohydrates also contain either an aldehyde or ketone compounds (freeman 2002). Carbohydrates are classified as simple and complex carbohydrates. The classification of carbohtdydrates is based on the chemical structure of the food and how fast it absorbs. Simple carbohydrates contains single or double sugars in their chemical structure whereas complex carbohydrates consists of three or more sugars (ahmed 2014). There are four major types of carbohydrates depending on their chemical composition.