health information management Essay

1222 Words Sep 11th, 2014 5 Pages
Biological Molecules
Experiment-Specific Questions
Experiment 1: Monosaccharide Test
1. Fill in the table below with the results from the monosaccharide test experiment, and your conclusions based on those results.
Results Monosaccharide Test
Solution
Initial Color
Color with Benedict's Solution
Color After Heating
Monosaccharide?
glucose solution

clear

blue

orange

yes water clear

blue

blue

no sucrose solution

clear

blue

blue

no fructose solution clear

blue

orange

yes

1. Benedict’s solution is added to white grape juice and heated. The color changes from blue to orange. Based on this result, what biological molecules are
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DNA General Concepts
1. How does gel electrophoresis sort DNA fragments? Electrical charges (positive to negative and so on). Also by size helps sort fragments as well. Small dna moves faster than larger ones do.

1. If each individual has such a small amount of DNA, how do the bands on the gel contain enough DNA to be visible? By restriction enzymes then amplified by polymerase chain reaction to make many to millions of copies of a single fragment.

1. Many genes only have a few possible alleles. For example, humans only have a few eye colors and only four blood types. How can DNA tests definitively identify individuals when many men have brown eyes or type A blood? The way this is done is by VNTR ( variable number tandem repeats) the length varies from person to person and the base pairing also differs as well.

Experiment-Specific Questions
Part 3: Analyzing the Results
1. List the distances traveled (in mm) for the bands in the DNA Ladder in the table below.
Remember, smaller fragments travel farther than longer ones, so the top-most band will be the 1,000 bp fragments while the bottom-most band will be the 50 bp fragments.
DNA Ladder
Band
Distance (mm)
50 bp 100 bp

150 bp 200 bp

250 bp 300 bp

400 bp 500 bp

600 bp 700 bp

800 bp 900 bp

1,000 bp

2.

1. The DNA fragments