Cycloomatostatin Lab Report

To begin the separation of the sucrose from the Panacetin, approximately 3 grams of Panacetin was transferred to a 125-mL Erlenmeyer flask. Added to the flask was 50 mL of dichloromethane to dissolve the Panacetin to aid in the separation. A fluted filter paper was used to filter the Panacetin and dichloromethane mixture by gravity using a glass funnel. The remaining filtrate was set aside than transferred to a separatory funnel and the substance on the filter paper was dried and weighed. The filtrate was extracted with 2 increments of 25-mL portions of aqueous 1 M sodium hydroxide. During this step the filtrate in the separatory funnel was shook and vented 3 times.

My unknown organism #6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. (3, 5) This bacterium Morganella morganii, was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide distribution, it is an uncommon cause of community-acquired infection and is most often encountered inpostoperative and other nosocomial settings. (2, 3) Morganella morganii infections respond well to appropriate antibiotic therapy; however, its

Can the angle of a fatal blow be determined by the locations of the victim’s bloodstains? I will be comparing multiple bloodstains to one another and measuring them, trying to determine the angle at which I struck the “victim” (a kitchen sponge). This practice, called bloodstain analysis, is used by forensic investigators to determine the cause, position, and even time of death. I believe that by using trigonometry, I will be able to establish the angles at which I hit each sponge. I will repeat the experiment multiple times, and each blow will be delivered from a different angle.

C.elegans are part of the nematoda family. The nematoda family are a group of worms that have unsegmented bodies and are usually round worms that can be free living or parasitic. C.elegans are a version of the worm that is non-parasitic, non-hazardous, and non-infectious. C.elegans have had their entire genome sequenced, this makes it easy for scientists to see what genes they are changing and how that will affect the organism. (Edgley and riddle lab, 2015). For this experiment chemotaxis was tested in the organism. Chemotaxis is defined by the following: The movement of organisms in response to chemicals, whether they be attracted or repelled by the chemical that is chosen. (Farley, 2012).

If the drug Fluvastatin lowers LDL cholesterol, then the people with high LDL cholesterol who take fluvastatin will have low LDL cholesterol levels.

Plants are common organisms, known for their aid in absorbing carbon and releasing oxygen into the airways. Plants, being living organisms, need both nutrients and water in order to stay healthy and strong (Gardening Know How, 2011). There are a large range of nutrients which come from the leaves and the soil of the plant, each with their

Ion channel activity in mouse bone marrow-derived DCs was analyzed utilizing whole cell patch-clamp experiments. As shown in Fig. 1, DCs express Kv channels. They presumably belong to Kv1.3 and Kv1.5 families (45, 56, 66). Pretreatment of the cells with rapamycin was followed by a gradual decline of Kv channel activity, an effect becoming significant with 50 nM rapamycin and 6 h of incubation (Fig. 1, C and D).

Catalytic Mechanism of the Serine Peptidase Chymostrypsin Chymotrypsin is a serine peptidase that preferentially cleaves proteins where the amine on the carboxyl side of the peptide bond contains a aromatic ring or a long hydrophobic chain that fits into a deep catalytic pocket on the enzyme. Thus, positioning the adjacent peptide bond into position for cleavage. This mechanism is propagated via a highly reactive serine residue, deactivation of which stops the enzyme from catalysing at all. The reaction occurs in two stages, a "short burst" phase and a "steady-state" phase (following a Michealis-Menton view of kinetics), the latter occurring at a much slower rate than the former.

The pH of saliva was 7 this means that it is neutral and there is a large amount mineral reserves, unlike when it is 6.25 to 6.75 which indicates that there is an excess of acid and that the there is a very low amount of mineral reserves and that on its own is not healthy. The saliva contributes to supporting the pH by two components. To begin with, the flow of saliva kills starches that could be utilized by microorganisms and evacuates acids created by microscopic organisms (Baliga et al, 2013). Second, acidity from beverages and nourishments, as well as bacterial activity, is neutralized by the buffering action of salivation (Baliga, 2013). The solution that was mixed with water and Biuret test reagents was clear because there was no protein presence,

Before the start of the lab, the column had already been prepared by our lab instructor. It was mounted on a ring stand and was fixed vertically for our use. The column contained the separation matrix consisting of Sephacryl beads with tiny pores which creates the molecular sieve. The gel was kept from drying by slowly adding small volumes of saline, and that is why we could observe a small layer of saline on the top of the gel before the beginning of our experiment. The bottom of the column was also securely sealed by a cap to prevent leakage of the solution which would lead to the gel drying.

After smoking marijuana, it takes weeks, even months for the THC metabolites to get off your system. One of the major ways the body flushes out THC from marijuana is via the kidney which is in turn, passed through your sweat and urine.

The purpose of the experiment was to observe the effect of the antimitotic drug cytochalasin D on the organization of actin in human fibroblasts. The human fibroblasts were treated with increasing concentrations of the antimitotic drug and viewed using fluorescence microscopy. With each increase in concentration of cytochalasin D, we successfully observed that the presence of stress fibers decreased. The treatment of 10µM of cytochalasin D indicated that higher concentrations consistently alter actin organization. In the highest concentration treatment, the human fibroblasts no longer had stress fibers, lamellipodia, filopodia, and F-actin organization was completely disrupted. This behavior was consistent across all cells on the slide. With

Chromatography is the term used to describe all the separation methods based on the distribution of compounds between two separate phases. TLC, an abbreviation for thin layer chromatography, is a chromatographic technique which is used to separate components of a mixture using a thin stationary which has an inert backing, this occurs as one phase is fixed on a plate (stationary phase) and the other phase is mobile and migrates through the stationary phase (mobile phase). During the chromatographic development process, the mixture to be separated is distributed between the stationary and mobile phases. (Spangenberg Bernd et al 2011). It is mostly used because of its simplicity, relatively low cost, high sensitivity and relatively fast speed of separation. TLC also functions on the same principle as all other chromatography techniques. A compound has different affinities for the mobile and stationary phases, and this affects the speed at which it migrates. The goal of TLC is to obtain well defined, well separated spots. (O. H. Lowry, 1951). In contrast to HPLC, many samples can be run at the same time on TLC. (Journal of Chromatography, vol 19 (2011)).

If the urine is light yellow color does not have to worry, but the pallor is not good for your health. Urine color does affect our health, to tell the color of urine have been here.

The urinary tract consists of two kidneys, two ureters, one bladder and one urethra. Its major function is to remove waste products from our bodies and help regulate the volume and composition of blood by the production and excretion of urine. This drainage system is carried out by nephrons, the functional unit of the kidney, in three subsequent steps: filtration, reabsorption and secretion. When blood enters the renal artery of the kidneys, the blood plasma is filtered by blood pressure forcing fluid across the glomerulus capillary membranes. Glomerular filtration creates a plasma-like filtrate of the blood. Filtrate moves through the proximal