Determination of the presence of Herpes simplex through Indirect ELISA
Priscilla Mariel M. Cadiz
Biology Department, De La Salle University, 2401 Taft Ave, Manila, Philippines
*Email: cadizpriscillamariel@yahoo.com
Sexually transmitted disease is currently one of the major health concerns in the world today. Herpes simplex virus is one cause of STDs. There are two types of HSV present namely HSV-1 which is often found in the mouth area and HSV-2 found at the genitals. Indirect ELISA was used in order to determine the presence of Herpes simplex among 3 different samples. Indirect ELISA involves two antibodies; the primary antibody which is bound to the antigen and secondary antibody bound to the primary antibody. Results produced by indirect ELISA produces better amplified signal. Results obtained showed that 1 of the 3 samples tested positive for HSV. Indirect ELISA however cannot determine what specific type of HSV is present in the sample. The cause of HSV-1 is the exchange of body fluids particularly obtained through kissing while sexual contact causes HSV-2. Though it rarely happens, there are possibilities that false positive or false negative results were obtained in Indirect ELISA. Interference may cause error.
1. INTRODUCTION
ELISA or Enzyme-linked immunosorbent assay is a biochemical technique used to check for the presence of a target protein of interest in an experimental sample and how much of protein is present (Voller et al, 1978). ELISA is considered an
Being in a relationship with someone infected with herpes can be of a challenge. It is only for the other partner to decide if he/she can live with the idea of it or not. Major problem is when a couple decides to take the relation on to another level and introduce sex in it. A good thing is to let the partner know about your condition before sex. In a dilemma of the disease you should give your partner a lot of encouragement
The ELISA test can also be used to detect antibodies that are produced in response to a specific antigen. Using information about how you completed this ELISA experiment, outline a procedure for testing for antibodies in the blood.
Generally this means a pleasing appearance is not a guide to character. In this case, we
Genital herpes the infected area is in the genital portion of the body. In a male it
The Enzymes linked immunoabsorbant assay (ELISA) is a commonly used biochemical technique, often used in immunology as a way to detect the presence of an antibody or antigen in a sample.
Herpes simplex is a viral infection, also known as HSV. The virus is highly contagious. It can be transmitted by close or direct contact with an infected person. The herpes appear anywhere on the body. The most common herpes simplex is oral herpes and genital herpes. The oral herpes cause cold sores and fever blisters around the mouth and on the face. The genital herpes are generally around the genital.
There is a old misconception Herpes Simplex 1 only infects "above the belt" by manifesting as cold sores. Herpes Type 2 was previously considered "below the belt" and caused genital lesions. However, the medical profession has found oral-genital contact can spread either, so that types of herpes have become virtually insignificant.
The ones I just mentioned are some symptoms of HSV. When someone is feeling that type of way, he or she should go for herpes test. The effects of HSV is that once it is contacted, it stays in the body forever, though it might be inactive. It can cause sores in the genital area. Some women have this sore in their sexual organ (vagina). The effect of HSV can make it easier for one to get HIV. It can cause problems in the nervous system, this is because once it is contacted, it will travel through the nerves and affect it. It can also cause pregnancy problems, this is when the disease (HSV) will go from the mother to the unborn child, causing fatal problem. If you are seeking for help on how to prevent HSV, ask your partner about his or her sexual history. Also ask your partner to be tested for genital herpes. Please don’t drive while intoxicated. I don’t know why someone would want to do that. To prevent HSV, one of the sexual intercourse partner should use a latex condom during every sexual contact, limit the number of sexual partners. People with HSV should abstain totally from sexual
Genital Herpes is a common STD caused by two viruses. The two viruses are herpes simplex type 1 and herpes simplex type 2. Any sexually active person can get it. Most people with the disease don’t experience symptoms. However, even though you can’t tell you have the disease, you can still spread it to sexual partners.
Genital herpes is one of the most common STDs in the U.S.A. Herpes is caused by the herpes simplex virus. There are two types of herpes simplex virus; herpes simplex virus-1, and herpes simplex virus-2. Both can cause sores on the genital area. Most people with genital herpes, (herpes simplex virus-2,) do not know they have it, since the symptoms are either nonexistent or very mild. Herpes simplex virus-2 most commonly causes cold sores, but can also be a cause of genital herpes.
In this article we will analyze the widespread of herpes and how HSV singles can engage in dating and networking like any other people out there.
Shingles like chickenpox may also occur in the mouth if the maxillary or mandibular division of the trigeminal nerve is affected. A rash will appear on the mucous membrane of the upper jaw, usually along the palate, sometimes along the gums of the upper teeth or even along the lower jaw (tongue or the gums of the lower teeth) respectively. It may show up alone or along with the rash along your skin. Like the skin lesions they will typically stay to one side of the mouth and they will distinguish themselves from oral blisters. They will typically show as 1-4mm opaque vesicles which will break down quickly to leave ulcers that will heal in about 10-14 days. Prodromal pain, occurring before the rash, could be confused with that of a toothache
This commercial would convince me not to have sex with a person who has herpes even if the person is taking medication to prevent the spread of herpes. The statics used in the commercial were very effective. For example, “One study found that up to 70% of people who had genital herpes got it from their partner when they had no signs or symptoms of an outbreak.” I did not think any aspects of the commercial were turn offs. A commercial like this is necessary, because some people might not know that a person can still spread herpes even if they do not have an outbreak or that there is no cure for herpes. The purpose of this commercial is to inform people about what herpes is and how Valtrex is used to prevent the spread of herpes.
Herpes is an infection that is caused by a herpes simplex virus (HSV).It is a sexually transmitted disease (STD) that affects sexually active individual. Most individuals with the virus don’t have signs. It is important to know that even if you don’t have signs of the disease, you can infect your sexual partners.
The patient from this case is an 18- year old female who experienced painful blisters on her genitalia, followed by a fever, body aches, headache and stiff neck. Since she has not had any known sexually transmitted diseases in the past, I believe she contracted Herpes Simplex virus type 1 (HSV1). The bister symptoms of this virus are often around the oral region but they can also be spread to the genitals. HSV1 is most often transmitted via oral-to-oral contact, but in this case it seems the patient had contracted it by oral to genital contact. It appears that the HSV1 virus caused the disease Herpes Meningoencephalits, which explains the rest of her symptoms. Meningoencephalitis is when both the brain and its surrounding meninges become inflamed