Determination Of The Presence Of Herpes Simplex

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Determination of the presence of Herpes simplex through Indirect ELISA Priscilla Mariel M. Cadiz Biology Department, De La Salle University, 2401 Taft Ave, Manila, Philippines *Email: cadizpriscillamariel@yahoo.com Sexually transmitted disease is currently one of the major health concerns in the world today. Herpes simplex virus is one cause of STDs. There are two types of HSV present namely HSV-1 which is often found in the mouth area and HSV-2 found at the genitals. Indirect ELISA was used in order to determine the presence of Herpes simplex among 3 different samples. Indirect ELISA involves two antibodies; the primary antibody which is bound to the antigen and secondary antibody bound to the primary antibody. Results produced by indirect ELISA produces better amplified signal. Results obtained showed that 1 of the 3 samples tested positive for HSV. Indirect ELISA however cannot determine what specific type of HSV is present in the sample. The cause of HSV-1 is the exchange of body fluids particularly obtained through kissing while sexual contact causes HSV-2. Though it rarely happens, there are possibilities that false positive or false negative results were obtained in Indirect ELISA. Interference may cause error. 1. INTRODUCTION ELISA or Enzyme-linked immunosorbent assay is a biochemical technique used to check for the presence of a target protein of interest in an experimental sample and how much of protein is present (Voller et al, 1978). ELISA is considered an

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