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Diffusion And Osmosis Lab Report

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The Effect of Concentration and Tonicity on Diffusion and Osmosis

Abstract:

In Exercise 5.2, the process of diffusion was observed using the enzyme amylase and a starch agar plate. It appeared that higher concentrations of amylase correlated with greater resulting ring diameters around the wells. For exercise 5.3, dialysis tubing was filled with a starch solution. This was used to simulate diffusion of solutions across a cell membrane. It was concluded that the tube gained weight after being submerged in a beaker, meaning that some of the water and iodine solution from the beaker diffused into the tubing. This shows that the beaker solution was hypertonic to the starch solution inside the tubing. However, none of …show more content…

These two processes are strikingly similar in their basic function yet also have a few key elements that set them apart from one other. In diffusion, through random motion, molecules spread out and move from an initial area of high concentration to areas of low concentration. This same concept applies to osmosis except that the molecules must diffuse through a semipermeable membrane. One important factor in osmosis in cells is tonicity. Animal cells function best in an isotonic environment. Being in too much of a hypertonic or hypotonic environment can have disastrous results for cells. Being in a very hypertonic environment can cause a cell’s cytoplasm to shrink and the cell to cease functioning. A hypotonic environment may cause a cell to burst. One part of the human body in which maintaining proper tonicity is extremely important is the blood. The blood must maintain approximately the same tonicity in order to prevent damage to its cells (ACTA Endocrinologica-Bucharest 2014). It does this through stimulating the release of hormones to the kidneys. If there is too little water in the blood, the kidneys are sent a signal to produce less urine and to instead absorb more water into the bloodstream. If there is too much water in the blood, the kidneys produce more, less concentrated urine and reabsorb less water back into the bloodstream. This lab investigates the basic principles of diffusion and osmosis from a biological standpoint. By observing the roles that concentration and tonicity play in diffusion and osmosis, a greater understanding can be gained in how these processes fit into the context of life’s cellular

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