The Effect of Concentration and Tonicity on Diffusion and Osmosis
Abstract:
In Exercise 5.2, the process of diffusion was observed using the enzyme amylase and a starch agar plate. It appeared that higher concentrations of amylase correlated with greater resulting ring diameters around the wells. For exercise 5.3, dialysis tubing was filled with a starch solution. This was used to simulate diffusion of solutions across a cell membrane. It was concluded that the tube gained weight after being submerged in a beaker, meaning that some of the water and iodine solution from the beaker diffused into the tubing. This shows that the beaker solution was hypertonic to the starch solution inside the tubing. However, none of
…show more content…
These two processes are strikingly similar in their basic function yet also have a few key elements that set them apart from one other. In diffusion, through random motion, molecules spread out and move from an initial area of high concentration to areas of low concentration. This same concept applies to osmosis except that the molecules must diffuse through a semipermeable membrane. One important factor in osmosis in cells is tonicity. Animal cells function best in an isotonic environment. Being in too much of a hypertonic or hypotonic environment can have disastrous results for cells. Being in a very hypertonic environment can cause a cell’s cytoplasm to shrink and the cell to cease functioning. A hypotonic environment may cause a cell to burst. One part of the human body in which maintaining proper tonicity is extremely important is the blood. The blood must maintain approximately the same tonicity in order to prevent damage to its cells (ACTA Endocrinologica-Bucharest 2014). It does this through stimulating the release of hormones to the kidneys. If there is too little water in the blood, the kidneys are sent a signal to produce less urine and to instead absorb more water into the bloodstream. If there is too much water in the blood, the kidneys produce more, less concentrated urine and reabsorb less water back into the bloodstream. This lab investigates the basic principles of diffusion and osmosis from a biological standpoint. By observing the roles that concentration and tonicity play in diffusion and osmosis, a greater understanding can be gained in how these processes fit into the context of life’s cellular
Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.
The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.
In this study we constructed we researched whether different sucrose concentrations affect the rate of osmosis. In order to do this, we constructed artificial cells out of dialysis tubing filled with 20% sucrose and 40% sucrose and weighed them every 10 minutes for 90 minutes. In doing so, we concluded that the higher the sucrose concentration, the faster the rate of osmosis.
Osmosis is one of the most common and important processes in biology. Countless processes within the human the human body rely on osmosis and other forms of diffusion, ranging from the exhale of CO2 out of the cell to the various reactions between organelles (Nave, n/d). Osmosis itself is the diffusion of water across the cellular membrane in and out of a cell. The direction of net movement is decided by the concentration of water and solutes on either side of the cell, always moving towards the side with the lesser concentration of water (Nave, n/d). The concentration is solutes is called the Tonicity, and this dictates the movement of osmosis across a membrane (Kahn Academy, n/d). To test this relation, potato cores were placed in different solutions with varying levels of sucrose and water. The common conception was that the increase in sucrose within a solution will cause osmosis to occur to a lesser extent between the potato and the solution. To measure this, the potatoes were weighted after a night of soaking in the solutions to test for a change in weight. It was the general conception that the greater the amount of sucrose within the solution, the lighter the potato will be.
The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient, the faster the rate of diffusion would be. To test this, dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific time intervals to measure the rate of diffusion of water in each different solution. The results illustrated that increased concentration gradient increases the rate of diffusion of water in the tubes. We concluded that as concentration of the
The purpose of this experiment is to observe the natural process of osmosis using drops of sheep’s blood in various saline solutions. Since the cell membrane is a selective membrane, the water goes in and out freely but a solute such as NaCl it cannot freely move in or out. Osmosis is a passive process that doesn’t require the input of energy to transport water molecules across a membrane. The channels that allow water to enter or leave a cell are known as aquaporin. Using aquaporin, water moves from a solution with a low solute concentration to a solution with a higher solute concentration. This process continues until equilibrium in solute concentration is established between a cell and its environment.
As we know that osmosis means diffusion or dispersion of water through a selectively permeable membranes from high concentration to a lower concentration and there are many different factors that can affect the rate of Osmosis such as temperature particle size and the size of concentration ingredient. As we know that higher temperature can cause osmosis to occur at much faster rate because a molecule are more likely to pass through the selectively permeable membrane quicker than they would at a lower temperature. In this experiment the sucrose molecule was too large to pass through the dialysis bag, while water molecule able to move freely in and out due to their small size. Lastly the congregation gradient can greatly affect the rate of osmosis
We filled bags of dialysis tubing with 20 ml of sucrose, NaCl, ovalbumin, and glucose and weighted them. After soaking them in distilled water for 10 minutes we measured them again. All of the bags gained water. Our hypothesis was that water will diffuse into the cells with ovalbumin, NaCl, glucose, and sucrose, but not the bag with distilled water. The independent variables were the different solutes in the dialysis tubing. The dependent variable was if the water would diffuse into the
Title Osmosis : How Water Diffuses Introduction The plasma membrane, the outside barrier of the cell is selectively permeable, allowing certain substances to cross more easily than others. When a cell is put in a solution, passive transport, diffusion, and osmosis occurs. During passive transport, diffusion of a substance takes place across a membrane without energy contribution, because of diffusion, the tendency for molecules to spread out. Net movement allows substances to move in one direction.
Conversely, animal cells thrive best in an isotonic environment, where there is no net change in their concentration. During the osmosis portion of the lab we created four dialysis bags of varying concentrations and place the bags in solutions of either 30% sucrose or reverse osmosis water (RO water). Two bags held RO water and the two other bags held either a 15% or 30% sucrose solution. We then observed and recorded the mass of the bags over the course of an hour to determine in which direction the osmosis occurred for each bag and at what rate.
Activity 3: Osmosis Osmosis is a specific type of diffusion. It is the movement of water molecules from an area of high water concentration to an area of low water concentration. Semipermeable membranes, such as the cell membrane, allow the unrestricted movement of water across the membrane, at the same time restricting the movement of solute molecules and ions. It has been estimated that an amount of water roughly equivalent to 250 times the volume of the cell diffuses across the red blood cell membrane every second. Despite this large movement of water molecules, the cell does not lose or gain water, because equal amounts go in and out.
Diffusion is the transfer of molecules from an area that has a higher concentration to an area that has a lower concentration. Osmosis is the diffusion of water. The purpose of this experiment was to study the process of osmosis. In order to test osmosis, eggs that had been soaking in vinegar were taken and placed in four beakers of solution with different levels of glucose. Using this experiment we were able to determine the rate of osmosis of different solutions, with various amounts of glucose, through eggs. In the results of this lab it was found that the eggs were either hypertonic or hypotonic and that the
Cells are always in motion, energy of motion known as kinetic energy. This kinetic energy causes the membranes in motion to bump into each other, causing the membranes to move in another direction – a direction from a higher concentration of the solution to a lower one. Membranes moving around leads to diffusion and osmosis. Diffusion is the random movement of molecules from an area of higher concentration to an area of lower concentration, until they are equally distributed (Mader & Windelspecht, 2012, p. 50). Cells have a plasma membrane that separates the internal cell from the exterior environment. The plasma membrane is selectively permeable which allows certain solvents to pass through
Osmosis is the net movement of water to balance the concentration between the extracellular fluid and intercellular fluid. When the ovis aries’ red blood cell is placed in a hypotonic solution (0,50 and 100 mM NaCl Concentration) the blood cells haemolysis because the concentration of the extracellular fluid is lower than that of the cells’ intercellular fluid meaning water is moved into the blood cells as water movement always occurs from the lower concentration to the higher concentration. This resulting in lysed or ruptured red blood cells hence the decreased Packed Cell Volume (PCV). In comparison when the ovis aries’ red blood cells is placed in a hypertonic solution (200 and 250 mM NaCl concentration) they crenate or shrivel due to the movement of water out to the cell because the concentration of the extracellular concentration is greater than that of the cells’ intercellular fluid. This accounts for the decrease in
Within every cell, a movement of a solvent occurs through a semipermeable membrane to equalize the concentration of solute on both sides of the membrane. The diffusion of water across the cell’s membrane down to its concentration gradient is called osmosis. In this case, the concentration gradient is the difference of density between one side of the cell membrane to the other. Since the cell’s membrane is permeable, particles can flow freely in and out of the cell, but the net flow will be strong in the direction of lower concentration until the system has reached a stage of equilibrium, the point at which both sides of the membrane are equal. In the