Effects Of Reoccurring Physical Contact On Bacterial Growth

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Effects of Reoccurring Physical Contact on Bacterial Growth in Human and Indoor Environments
Erick Dominguez
Biology 1108
Wednesday 5:32p.m.

Abstract: The knowledge of the existence of bacteria can be traced back ages, however their existence can be traced to the very start of planet Earth. The project focused on surfaces with constant physical human interaction and the growth of bacterial colonies in indoor environments. The process centered on the use of Aseptic technique to collect samples of bacterial colonies on the locations we hypothesized contained the highest number of bacterial colonies. Three trials of each were conducted and a control group was used in which it was sterile water. The results proved our hypothesis
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Not only do these pose a threat of illness but identification of the pathogen would prove timely and pose a serious threat to the public.
Therefore, it is vital to research the number of bacterial colonies located in common everyday surface faced with constant human contact. We hypothesize that the door handle will house more bacterial colonies than the those of the elevator door due to the constant physical interaction with the door handle through various means. The independent factor is this experiment is the selection of the location and the dependent factor is the number of bacteria colonies.

Materials and Methods
Four different bacterial species were incubated in prepared in nutrient–rich media Tryptic Soy Agar (TSA) plates. While preforming Aseptic technique we will use 1 capped test tube of sterilized water and sterile cotton swabs to extract Bacteria from the surfaces. A sterile cotton swab was dipped into the sterile water solution and carefully swabbed onto the TSA plate in a Z motion, to place the bacteria onto the plate. Foremost the cotton swab was dipped into the sterile water test tube and Z streaked on a TSA plate, this was the control of the experiment and to determine if contaminates were already located in the test tube. Afterwards the same procedure was repeated in different sources to obtain three trials of each location for a total of two different locations followed by the control. The bacteria were incubated by the TA at 37 ˚C for 2-3
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