Effects Of Reoccurring Physical Contact On Bacterial Growth

The identification of unknown organisms carries important ramifications that can be applied to many real world scenarios. In keeping with quality assurance beverages, food, cosmetics, and other products are frequently inspected for contaminants resulting from a presence of pathogenic bacteria. In medicine, a physician’s diagnosis and consequent treatment is largely determined from samples collected from infection sites that have been analyzed using microbial tests.

The purpose of this experiment is to investigate the effects of various household products may have on bacterial growth. The bacteria that was chosen as a test subject is Micrococcus luteus, which is generally considered a harmless bacterium unless the host has a weakened immune system. Among the household products that a set of groups will test are common cleaning and disinfecting products such as rubbing alcohol, Betadine, Germ-X, Neosporin, liquid hand soap, and Lysol cleaner. In the experiment, the bacterium, Micrococcus luteus, is to be swabbed and grown in a Petri dish divided into four sections with agar in each. After that, a small circle of thin paper is soaked in the four household items. Then, a piece of parafilm

(Harley, 2011, pp. 102-103) Sucrose and lactose serve as a fermentable carbohydrate sources which promote its growth of fecal forms and provide color change differentiation. (Harley, 2011, p. 104) Therefore, if E. coli is being platted on the EMB, after plate’s incubation period, it should produce a green metallic sheen on the agar due to the bacteria being a strong fermenter. My unknown bacteria tested positive for growth, and agar was fermented reddish burgundy in color. Subsequently, the unknown bacteria was later inoculated with a sterilized loop into the liquid tryptic soy broth and incubated at an appropriate temperature. Its results were used for proper identification of turbidity, spots and flocculation. (BD™ Tryptic Soy Broth (TSB), 2008) The results of the unknown were cloudiness and some settlement on the bottom of the tryptic soy liquid. The next step was conducted to find out if all the bacteria, as well as the unknown culture, required oxygen for growth, varying from an aerobic environment, where bacteria needs oxygen to grow, to facultative anaerobic environment, where bacteria will grow either with or without oxygen but better in its presence. All the bacteria, along with the unknown, were separately inoculated and tested. My unknown culture results tested positive for growth in facultative anaerobic environment. In the next sequence of lab experiments, the results of unknown bacteria were determined by glucose fermentation,

When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.

9. After the 48 hour time period the inhibition of the Escherichia coli bacteria around the disinfectant disks was measured. A ruler was used to measure the clearing from the edge of the disk to the perimeter of the clearing. Irregularities in the clearing were not included in the area of clearing.

A urea hydrolysis test was performed. A nitrate reduction was performed. The bacterial unknown was grown on Kigler 's iron agar media, MSA media, soy agar media, PEA agar media, EMB media, and SIM medium media. A gram stain was performed. A methyl red test was performed. A Voges-Proskauer test was performed. A citrate test was performed. A motility test was performed. A gelatin hydrolysis test was performed. A liquid broth agar was cultured to determine if there was use of oxygen.

Thoroughly washing your hands can be one of the best ways to prevent the spread of pathogens that cause illnesses. Our bodies have several lines of defense to fight infections. It is very important to do what we can do to avoid the pathogens from entering our system in the first place. Our hands pick up microscopic pathogens from objects that were touched by people who may not have washed their hands very well. When we forget to wash our hands or don’t wash them properly, we can spread pathogens from our hands to our internal systems by touching other parts of our body. During this lab activity, my partner and I will test the effectiveness of different hand-washing times, techniques, and

The article titled, “ ‘Harmless’ Things You Should Really Wash Your Hands After Touching” by William Harris, is that every day of people's lives they get germs from all the things they touch.

The lab results show that the more the human contact frequency increases, or the more the number of people who touch a surface daily, the more the approximate number of bacterial colonies and colony morphologies increase (Figure 2) (Figure 4) (Figure 5). The lobby door knob is the surface with the highest human hand contact frequency and displays the most bacterial growth and diversity. For example, on each of the days, the lobby door knob shows the most approximate number of colonies (Table 1). It shows 50 colonies on Day 1, 75 on Day 2, and 116 on Day 3 (Table 1). For the average number of bacterial colonies, the lobby door knob is the surface that has the highest, at an average of 80 colonies (Table 2).

Since their biological and medical application is important in discovering possible diseases, cures, and evolutionary relationships, the prevalence and spread of bacteria and fungi were studied based on the species richness, diversity, and relative abundance. Specifically, ten cell phone surface samples were taken and measured based on two parts: the outside of the phones and the inside of the cases, and the front of the phones and the back of the cases. In this lab, the front of the phone and the back of the case was hypothesized to contain species with more richness, diversity, and relative abundance. In order to test the hypothesis, ten Petri dishes were divided into two sides, five dishes meant for the front of the phones and the back of

Some brainstorming was done to determine the lineup of the bacteria on two Petri dishes. Next, the experiment started with the labeling of the two Petri dishes. This was followed by a mistake that occurred, which was corrected with an orange dry-erase marker. Then our hands were sanitized with hand sanitizer, and was followed with bacteria collecting. The bacteria was collected, from the silver-ish, doorknob on the inside of the door near room B216 (with evacuation plan), and the beginning part of the handrail at the right found near the stairs of the B building opposite to Luck Hall. After that, the process was

This experiment illustrates the importance of handwashing and proves that hand washing is worth it. Since our hands are constantly coming into contact with ourselves and others, touching surfaces, grabbing objects, being sneezed into, etc., keeping our hands clean is one of the most effective, yet simple way we can take to avoid getting sick and spreading germs to others. Many diseases and conditions are spread by not washing hands with soap and clean, running warm water. “The human skin is a host to anywhere between 10,000-10,000,000 bacteria per square centimeter and since health care providers come into contact with pathogenic bacteria by being engaged in patient care, hand washing can reduce the risk of spreading diseases (page 3).” The objective of the experiment is to test the effectiveness of hand washing and demonstrate normal flora. This report presents the procedures and materials for the experiment, the experiment's results, and an analysis of those results.

Dr. Venkateswaran and other scientists of NASA Jet Propulsion Laboratory, Caltech reported in a recent study that human presence in closed habitats, that someday may be used to explore other places, is linked to the presence of fungal community (mycobiome) on the surfaces inside the habitat. Inflatable Lunar/Mars Analog Habitat (LMAH) is utilized to mimic the conditions of International Space Station and other planets as possible human habitat. It is designed to know the physiological, psychological and behavioral changes in humans in a restricted environment. Subjects were contained inside the ILMAH for 30 days, except for the exchange of filtered air between the indoor and outdoor environments. Samples collected from eight locations at four

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.

This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.