The Effect of Increasing the Concentration of Dettol Antiseptic Liquid on the Growth of E.Coli

“Ajax Spray ‘n’ Wipe” specifically states that it kills E. coli. This is because of the lactic acid in the spray, which is used as a natural antibacterial agent.” ("Colgate-Palmolive | Product Ingredients"). Lactic acid kills unwanted bacteria especially in food which is where E. coli is often found and transmitted, of which meat is the most common cause. Vinegar was chosen as a substance because “it can be used as a bleach alternative” (“Disinfectants: A Guide to Killing Germs the Right Way”) and bleach kills E. coli. It is also used as an everyday cleaning product. The last substance chosen was salty water because it is thought that salt kills bacteria. Much of the world’s population has access to salt and if the experiment proves that salt kills E. coli it would be a helpful factor for further treatment of the

(Biology Dept.). 0.1 ml of E.coli K or 0.1ml of E.coli B was added to the 10 fold dilution. Using soft agar technique, the growth media mixture with E.coli was plated and incubated.

E.coli outbreaks have steadily grown over the last few decades. An expansion in big farming has led to E. coli not only being found in meat, but vegetation as well, due to waste runoff. This has increased our need for adequate antibiotics that can fight bacteria, like E. coli. The best way to pinpoint which antibiotics work is by measuring their ability to create antimicrobial agents or zones of inhibition. When a paper disc that has been saturated in an antibiotic is inserted in a solution of E.coli and medium, the zone of inhibition will be noted as the clear ring that forms around the disk. The antibiotics efficacy is then determined by measuring each disk zone of inhibition, and comparing these measurements to the zone measurements of an untreated specimen. If an antibiotic is to be deemed sufficient for treating E. coli it should show a zone of inhibition that is at least double the size of the untreated specimen.

If the solution is okay, you will then completely immerse the cleaned implements in an appropriate disinfection container holding the EPA-registered disinfectant for the required time. This will be at least ten minutes or the time stated in the manufacturer’s instructions. It’s always best to set a timer than it is to guess. Remember to open all hinged implements before immersing them in the disinfectant.

For this experiment, a non-virulent strain of Escherichia coli was used to illustrate differences in growth rates for different temperatures. The culture was created by placing 35.0 ml of L-Broth into a 125 ml flask and adding 0.025 ml of an overnight bacteria culture. This culture mixture was divided and placed into 3 shaking incubators that were kept at 25˚C, 30˚C, or 37˚C (Timmons 2004).

The aim of this experiment was to observe the effect different concentrations of Dettol had on the growth of S. albus. The highest concentration of Dettol (100% - D) was predicted to be the most effective solution to kill and stop the growth of this particular bacteria. This can be justified as S. albus is gram-positive; easier bacteria to kill, developing resistance slower than a gram-negative. Therefore, the highest concentration of antibiotic should be – and was – the most successful in changing the pH levels, and killing S. albus. Through discussion and analysis of the results collected through the experiment, it can be concluded that there is higher chance of killing and the growth of this particular bacteria if 100% Dettol is used.

Escherichia coli (E. coli) is a bacteria that commonly live in the gastrointestinal tract of humans making it relevant to our population. The majority of E. coli strains is harmless, but it has the potential to form a potent strain that possesses serious health risks. When encountering this potent strain, one can experience health problems such as diarrhea or urinary tract infections. It is important to be able to determine when someone is being affected by the harmful strains of E. coli and how antibiotics affect them in order to keep the strain from cultivating further. Current scientific knowledge about the relationship between E. coli growth and antibiotics supports the idea that with the presence of antibiotics, the growth of E. coli is

The results of this investigation has a large degree of significance to the real world. Many people use disinfectants, such as the ones used in this investigation, to clean their outside environments (the outside world) and their inside environment (their body) from the E. Coli disease. Investigating and determining which disinfectants can continually clean

Throughout the antiseptics experiment, three different hand sanitizers were tested; One Step, Bath and Body Works, and Purell. Among these three different hand sanitizers, after observing our petri dish, we were able to figure out which one was the most effective in inhibiting bacteria. The sanitizer which was most effective in inhibiting bacterial growth was C, Purell. We are able to see that the Purell sanitizer which was placed on the disc and had diffused onto section C, was the most effective when inhibiting bacterial growth of observations that included of the measurement of the zone of inhibition and also research upon the active ingredient with the sanitizer. We also observed that the Purell sanitizer (in section C) was the most effective because the zone of inhibition was the largest at the end of the two days from all of the other sections and sanitizers. The

A 10-Fold Dilution Series process was necessary to examine the bacteria further. The materials needed included: one Bunsen burner, one inoculation loop, one BIC ® Multi-Purpose lighter, three sterile LB Agar Petri dishes, one bottle of sterile saline solution, one pipette gun, one canister full of sterile glass pipettes, a medium sized test tube track, and one plastic tub of medium glass test tubes. Next, one participant’s 10-Fold Dilution series was performed and three lines of ten medium test tubes were placed in the medium test tube rack. 9 ml of saline were put into each test tube. The gas for the Bunsen burner was switched on and lit it using the lighter. The inoculation loop was sterilized with the flame for a few seconds and then removed

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment

The target of section number one in the lab is to develop microbes on a petri dish to show bacterial amplification in distinctive places. The reason of why I did this was to outwardly observe which territory develops the largest and minimum microbes throughout the span of around eight days. Once the experiment was over, I presumed that the lavatory seats were the most dirty in comparison to the rest. The origination that the restroom seats being the most dirty was invigorated by the examination. The motivation behind section number two in the lab is to exhibit how cleaning supplies influence bacterial amplification. I utilized cleaning supplies, for example, purell and hand cleanser. I also utilized Tetracycline and 409. Following 8 days of outwardly inspecting the antibacterial operators do their work, I found that the Tetracycline did kill the most microorganisms on the petri dish, which braced my theory.

The importance of this study is that it can help us with certain bacterial strains which tend to live on under high concentrations of organic solvents such as phenol (Isken; 1998). Research in this field revealed that high buildup of such organic solvents are not only damaging by themselves, but found that bacteria such as E. coli, which have entered near the cell membrane, undergo ATP synthesis (use phenol to metabolize) and continue to survive longer than normal (Isken; 1998). We can now use this information to help us avoid bacterial infections by eliminating the thriving environment for the bacteria, which is a factor of great importance.

This study is to examine the effects of different types of disinfectants by disk diffusion method using common agents such as Betadine, Clorox, Crest, Kiss My Face, Listerine, and Lysol. Wound care and general cleaning of home surfaces is a part of daily activity for many of the general population. Knowing which agents are effective against the bacteria that most commonly cause infection, disease, and illness allows educated decisions on which agents to use. We compared the bactericidal effectiveness of 6 common agents widely used by the general

The bactericidal effect of aqueous solutions increases steadily as the concentration approaches 100% v/v. Isopropyl alcohol is ineffective against bacterial spores.