Enzyme Lab Conclusion In this lab three hypotheses were tested. If the catalase Solution is added then, the H2O2 will be broken down into harmless byproducts/ there will be an increase in temp compared to the control, if the boiled catalase is added to the H2O2, then there will be no significant increase in temp compared to the control, and if the catalase with acid is added to the H2O2, then there will also be no significant temperature increase compared to the control. The first hypothesis of, if the catalase solution is added to the H2O2, then there will be an increase in temp compared to the control, was proven correct. The control(the H2O2 with no catalase added) stayed at a constant temp of 23.5 degrees celsius throughout the 5 minutes …show more content…
This is different than the H2O2 with catalase which started at 23.5 degrees celsius before the catalase was added, but then increased temperature to a peak of 33 degrees celsius and after 5 minutes to a final temp of 29.5 degrees celsius. This is a proof that a chemical change occurred meaning the H2O2 was broken down using the enzyme Catalase, which sped up the chemical reaction. This chemical reaction is not seen for the second hypothesis, if the boiled catalase solution is added to the H202, then there will be no significant increase in temp compared to the control. That means that the hypothesis is correct, which is supported by the results. Compared to the control, which had a steady temp of 23.5 degrees celsius, the boiled catalase when added also stayed in the range of 23.5 degrees celsius. This means that no chemical reaction occurred because there is no significant increase in temperature as seen with the chemical reaction tested in hypothesis one. This is also true for hypothesis three, if the catalase with acid is mixed with H2O2, the there will no significant increase of temperature …show more content…
Just like with the control and the H2O2 with the boiled catalase, which both stayed at a constant temp of 23.5 degrees celsius, the catalase with acid also stayed in the temperature range of 23.5 degrees celsius. Since there was no temperature change, then there was no chemical reaction as proven by hypothesis one seen when the regular catalase was added to the H2O2. The different temperatures were direct result of the catalase used. A catalase is a specific enzyme found in animals (specifically an animal liver was used in this lab) exerted to speed up the breaking down of Hydrogen Peroxide (H2O2) into less harmful byproducts such as water and oxygen in the presence of light. In the first hypothesis tested using regular the catalase the temperature change indicates that the chemical reaction occurred swiftly in the 5 minutes, breaking down the H2O2. The constant temperatures found in the testing of the second and third hypotheses proves that there was no chemical reaction breaking down the hydrogen peroxide. This is because the enzyme was denatured. When an enzyme is denatured it means that the protein changes its shape due to outside stress on the
In this lab or experiment, the aim was to determine the following factors of enzymes: (1) the effects of enzymes concentration the catalytic rate or the rate of the reaction, (2) the effects of pH on a particular enzyme, an enzyme known and referred throughout this experiment as ALP (alkaline phosphate enzyme) and lastly (3) the effects of various temperatures on the reaction or catalytic rate. Throughout the experiment 8 separate cuvettes and tubes are mixed with various solutions (labeled as tables 1,3 & 4 in the apparatus/materials sections of the lab) and tested for the effects of the factors mentioned above (concentration, pH and temperature). The tubes labeled 1-4 are tested for pH with pH paper and by spectrophotometer, cuvettes 1a-4a was tested for concentration and cuvettes labeled 1b-4b was tested for temperature in four different atmospheric conditions (4ºC, 23ºC, 32ºC and 60ºC) to see how the enzyme solution was affected by the various conditions. After carrying out the procedures the results showed that the experiment followed the theory for the most part, which is that all the factors work best at its optimum level. So, the optimum pH that the enzymes reacted at was a pH of 7 (neutral), the optimum temperature that the reactions occurs with the enzymes is a temperature of 4ºC or
The hypothesis is that catalase activity will increase exponentially with higher concentrations of hydrogen peroxide until all catalase active sites are filled, in which case the
peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen
· I predict that the enzyme will work at its best at 37c because that
Students will be observing normal catalase reaction, the effect of temperature on enzyme activity, and the effect of pH on enzyme activity in this experiment. The enzymes will all around perform better when exposed in room temperature than when it is exposed to hot and cold temperatures. This is based on the fact that the higher the temperature, the better the enzymes will perform, but as the temperature reaches a certain high degree, the enzymes will start to denature, or lose their function.
Temperature can affect the reaction of catechol oxidase by speeding up or slowing down the reaction. I was able to see what happened to the absorbance after changing the temperature of the catechol oxidase solution. I did this by heating and cooling the solutions to measure the absorbances in hot, cold, warm, and room temperature. Then the data was compared to see how the temperature effected the solution. The catechol oxidase solutions reacted best in room temperature (twenty-three degrees Celsius) and the worst in the cold (zero degrees Celsius). I concluded that temperature really does affect the way catechol oxidase reacts.
These results show how temperature of extreme high, or low affects enzyme activity. The highest rate of enzyme activity occurred at 37 Cº. Anything that was hotter or cold than 37 Cº slowed the reaction rate. As I thought, 100 degrees would denature the enzyme, and that was the case. The data provided shows exactly what temperatures enzymes work best, and worst. The objective was achieved as we discovered the different reaction rates under different temperatures. The results are reliable, as we know enzymes do not work well when under extreme heat or denaturation occurs. What I learned in this experiment was that enzymes don’t work well under cold temperatures because they tend to move slower. My hypothesis did not quite match, because I thought they work best at lower temperatures.
There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.
The hypothesis tested in this experiment was, if the temperature of enzyme catalysis were increased, then the reaction rate would increase, because enzyme-catalysis reacts by randomly colliding with substrate molecules, and the increase in temperature increases the speed of collision or reaction rate. The final data collected for the experiment was positive with my hypothesis. The coffee filter, covered in potato solution, sank and rose at a faster pace in the hydrogen peroxide when the temperatures were raised.
The purpose of this investigation is to discover the effect of pH on the activity of catalase, an enzyme which plays the integral role of converting hydrogen peroxide into water and oxygen, and discover which pH level it will work at the most efficient rate (the optimum). The original hypothesis states that that the optimum would be at a pH is 7, due to the liver, where catalase usually resides, being neutral. The experiment consists of introducing the catalase to hydrogen peroxide, after exposure to certain solutions; hydrogen peroxide, water and hydrochloric acids, all containing the adjusted pH, and measuring the height of froth formed, an observable representation of the activity of the enzyme. The final data indicated that
The prediction of minimum activity occurring in 0° C and a maximum activity in 37° C was also supported. Through this lab it is easier to see when enzymes are at their weakest and their strongest. Within the body an enzyme is most efficient at a comfortable 37° C. For Catalase, its job in a perfect environment includes breaking down millions of hydrogen peroxide molecules every second (rcsb.org). As the temperature was increased, the reacting molecule H2O2 gained more kinetic energy, increasing the rate of bonds with the Catalase molecule and oxygen production.
For the next test the results were: in the incubator (37°C) had 75mm of bubble height. The test tube that was in the ice bath (1°C) had the largest reaction with 80 mm of bubble height. The test tube that was in boiling water (95/98°C)had no reaction. With these results we now know that our hypothesis for temperature change was somewhat right. We said that by increasing the temperature the catalase would begin to denature and have a very small reaction, which was correct.
This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.
The purpose of this experiment was to test the effects that temperature, pH, and substrate
What are the effects of different temperatures on the decomposition of Hydrogen Peroxide with the Enzyme Catalase. Different enzymes work best at different optimal temperatures, and with this experiment we hope to discover which temperatures are unideal for Catalase. The substrate is H2O2 which binds to the active site of the enzyme Catalase. The reaction in question is as written below: