Essay on Enzyme Lab Report

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AP Biology Lab: Catalase (Enzymes)

Abstract In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes.
Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.
Four AP Biology classes performed this experiment and the data presented in this report will reflect the averages of said classes. In the later discussion sections, it will become
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The instant the disc hit the bottom, a timer was started. The time measured was that from the time the disc reached the bottom of the beaker until it touched the surface of the hydrogen peroxide. This measurement is that of the activity rate of the enzyme. Finally the procedure was carried out for a second trial. This procedure was carried out for all four of the conditions being tested and the rates were recorded in respective data tables. An average rate of all four classes, that performed the experiment, was then graphed. Said graphs and data tables are shown in the subsequent section.
Data and Analysis Rate vs. Enzyme Concentration 2A 3A 4A 4B Average
100 0.081 0.654 0.057 0.13 0.201
80 0.087 0.109 0.043 0.173 0.103
75 0.181 0.057 0.05 0.127 0.104
60 0.095 0.038 0.047 0.111 0.073
50 0.131 0.034 0.041 0.098 0.076
25 0.077 0.014 0.028 0.067 0.047
10 0.064 0.011 0.026 0.042 0.036
0 No Rx No Rx No Rx No Rx No Rx

As the enzyme concentration increased so too did the activity rate. The relatively level areas show where there was substrate depletion.

Rate vs. Substrate Concentration 2A 3A 4A 4B Average
0% No Rx No Rx No Rx No Rx No Rx
0.10% 0.023 0.008 0.01 0 0.01
0.20% 0.025 0.019 0.01 0.011 0.016
0.30% 0.04 0.021 0.014 0.015 0.023
0.50% 0.058 0.018 0.02 0.024 0.03
0.80% 0.041

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