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Gastrointestinal Smooth Muscle Report

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Smooth muscle founded in the gastrointestinal tract of the Northern leopard frog, Rana pipiens, contracts through myogenic activity. However, neurotransmitters from the parasympathetic and the sympathetic branches of the autonomic nervous can influence the strength of these myogenic contractions. To explore myogenic activity under the influence of the autonomic control, we examined the contractile activity with and without additions of chemical compounds associated with the autonomic nervous system. We also wanted to observe contractile activity under the influence of mechanical stimulus by manually stretching the gastrointestinal smooth muscle. With the addition of acetylcholine and eserine a significant increase in contraction amplitude and…show more content…
The addition of acetylcholine caused a significant increase in contraction amplitude from 0.00983 + 0.00109 N (N=3) to 0.0740 + 0.00807 N (N=3) (Fig. 1). It also caused a significant increase in contraction duration from 17.3 + 1.44 s (N=3) to 47 + 10.5 s (N=3), the maximum duration observed (Fig 3). The second compound added was eseine. The amplitude of these contractions significantly increased to 0.117 + 0.0126 N (N=3), the maximum amplitude observed (Fig. 1). The frequency of contractions also significantly increased from 1.67 + 0.577 (N=3) to 3 + 0 (N=3), the maximum frequency observed (Fig. 2). Moreover, contraction duration significantly increased to 41.8 + 5.01 s (N=3) when compared to the baseline (Fig. 3). There was a third and fourth treatment that added the chemical compounds atropine and epinephrine respectively, but due to the flat lining of the gastrointestinal smooth muscle, measurements were not obtained. After the manual stretch of the gastrointestinal smooth muscle only one contraction was observed. The amplitude of these stretches decreased to 0.00167 + 0.000404 N (N=3), the minimum amplitude observed
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