All chemicals and reagents were obtained from Sigma (Sigma-Aldrich). A glycerolated Tris-lactose egg yolk extender was freshly prepared for dilution of semen as described by El-Bahrawy et al. (2006). Briefly, the extender was composed of 0.25 Molar Tris buffer (3.25 %, w/v), lactose (5.5 %, w/v), citric acid (1.67 %, w/v), glucose (1 %, w/v), and was further supplemented with egg-yolk (20 %, v/v) and glycerol (3 %, v/v) to reach a final pH of 7.4 and 0.351 osmol/kg osmotic pressure. The glycerolated diluent was added to the semen specimens in a one-step dilution technique.
In order to generate a bicyclic lactone in this experiment, a Diels-Alder adduct was produced. The bicyclic lactone to be generated was cis-1,3,3a,4,5,7a-Hexahydro-5-methyl-3-oxo-4-isobenzofuran-carboxylic Acid and was produced using a Diels-Alder reaction. The product was also analyzed quantitatively using percent yield. To prepare the Diels-Alder adduct 0.40 g of 2,4-hexadien-1-ol was added to a flask, then 5.00 mL of toluene and 0.40 g of maleic anhydride were added to the flask in that order. The mixture was warmed and stirred to induce a reaction. The reaction progress was monitored using a TLC plate with 30:70 hexane used as the mobile phase and silica gel as the stationary phase. The TLC plate revealed a new spot for the crude product, indicating the reaction had begun.
One of the main problems you do not want in a lab is a contamination issue, especially when a test is time sensitive. This situation came up when we received and analyzed the results of one of our major and most extensive tests. At the time, the lab was low staffed and I was the most familiar with the test. I believed I was sufficiently experienced with time management and knowledge that I could successfully determine the root of the contamination in tandem with completing current tasks assigned. I evaluated all possible angles of the test and realized that there were multiple factors and various opportunities that could have caused this to happen. I decided to channel most of my energy towards braking down the steps/phases and examined any
As shown on the table of results, the most accurate results were obtained for the sample of water, as it had relatively low percentage differences. On the other hand, Glycerin had the worst results with percent differences over 30%. Motor Oil was in between, with its percentage differences being roughly between 12% and 19%.
To determine what is present in the unknown, if the unknown and known substances spotted on the same TLC plate give spots in identical locations and R_f values are the same, then they may be identical. Ultraviolet light (UV) and iodine vapor are two techniques that can be used to visualize the nitroanilines in a developed TLC plate. In the case of UV light, because the absorption of UV light is found in organic functional groups containing chromosomes, such as nitroanilines, this technique can help visualize colorless compounds. In addition, iodine vapor can be absorbed by most organic compounds substances like nitroanilines except alkanes and alkyl halides. Once absorbed, the substance will appear as brown spots on the plate. A TLC plate can
In several experiments,65-67 Moench also depicted a wide picture of abnormal sperm morphology and attempted to provide a reference value for evaluating the capacity of fertilization of men. According to Moench, semen with morphologically abnormal spermatozoa up to 20% would be normal. Semen between 20 and 23% would have decreased fertility and value >25% they were compatible with sterility.
Conducting this lab there was many parts that determined the results of each section. Beginning the lab we were provided a weak acid, which was labeled as the Glycine, which contained a pka of 9.5. A weak acid is recognized as an acid that ionized partially. With a weak acid it makes it similar to the weak base due to that also being only partially ionized rather than completely ionized. During the experiments we had Solium Glycaine as our weak base. With the use of our weak acid and our weak base we were able to develop an equal mixture of both solutions together to create our buffer. The buffer developed was both the Glycaine and the Sodium Glycaine equally mixed together. The use of a buffer is what is used to adjust the value of
It’s my son’s 6th birthday and we are trying to bake a cake. I need the volume of the cake to be 451 cubic inches. The cake is in the shape of a rectangular solid. I want the cake to be four inches longer than the width of the cake and the height of the cake to one-third of the width. What should the dimensions of my cake pan be?
Every reaction is categorized as exergonic, endergonic, or at equilibrium. In order for the reaction to be categorized the value of G needs to be calculated. The G value is known as the Free Energy Change (Campbell pg. 147). If a reaction releases energy and is spontaneous, then it is considered exergonic; and if a reaction absorbs/requires energy, then it is considered endergonic (Campbell pg. 147). When a reaction is exergonic, its G is less than 0, negative, because that means that the potential free energy of the products is less than that of the of the products, while, in endergonic reactions, the free energy of the products is more than that of the reactants, making the G positive, greater than 0. However, when the free energy of the
Immerse slides in Schiff’s Reagent for 15 minutes at room temperature (18-26°C). Sections will become a light pink color during this step.
The objective of the study is to investigate the effect of Selenium and Vitamin E on the semen of teddy bucks. For this purpose 12 Teddy bucks will be taken for the experimentation. 12 Teddy bucks will be divided into four groups such as A, B, C and D. Each group will contain 3 teddy bucks. First group A will be kept as a control group. The second group B will be injected with Sodium Selenite @ dose rate of 0.10 mg / kg. Third group C will be injected with vitamin E @ dose rate of 1.35 IU/Kg. While the forth group D will be injected with Sodium Selenite @ dose rate of 0.10 mg / kg and vitamin E dose rate of 1.35 IU/Kg. Experimental animals will be injected twice a week for 2 months. Semens will be collected every
Glutaraldehyde concentration is indispensable factors that have effects on the enzyme immobilization process. So, glutaraldehyde concentration was varied from 0.25- 2%. As displayed in Fig. 7, the remaining activity of AChE increased by increasing of glutaraldehyde concentration to record the maximum activity at 1%. Then, as increasing of glutaraldehyde concentration, the remaining activity decreased. With glutaraldehyde concentration increasing, the activated amino groups of chitosan increased, the immobilized amount of the enzyme also increased. Whilst, the increasing of glutaraldehyde solution concentration too much, the excessive activated groups with aldehyde, leading the enzyme molecules to form a multi-point binding with the carrier
The objective of this experiment was to calculate the lower concentration limit of detection and detection threshold through instrumental analysis and human psychophysics, respectively. These values were then statistically compared to determine the superior method and inherent advantages/disadvantages were reviewed.1 Gas chromatography (GC) is a critical tool in a wide array of applications relating to food analysis, for the separation of various compounds (especially volatiles) within a mixture is typically essential in the characterization of inherent aroma compounds and undesirable defects of a given food product. Therefore, accurate quantification/qualification using GC/human psychophysics is a crucial aspect of the research and development
Vitamin C, or ascorbic acid, is a well-known supplement that is essential to the human body. Vitamin C helps grow and repair body tissue, make collagen, heal wounds, and strengthen bones and teeth. Unfortunately, the body does not produce this vitamin itself, therefore it must be obtained from another source. Vitamin C is present in significant amounts in both fruits and vegetables. These foods are commonly pasteurized – a process that applies heat to destroy pathogens that cause spoilage in food. Pasteurization is great for preserving foods, but its effects on the food’s contents are important to consider. This process could affect the levels of ascorbic acid in the foods being consumed for their vitamin C content. Specifically, orange juice, one of the most popular sources of vitamin C, is going to be used to examine the effects of pasteurization on ascorbic acid levels in this experiment.
The presence of glucose in the body of us and other animals is essential as it undergoes glycolysis to produce essential energy-rich ATP which is vital to keep the muscles, neurons, red blood cells and other energy-demanding cells operating. When an intake in food occurs, plasma glucose concentrations increase which stimulates the release of insulin from the beta cells in the pancreas, this causes an increase in glucose uptake by the cells and initiates glycogenesis, the process of turning glucose into glycogen in the liver and the muscle cells ().
Manage Engine free Wi-Fi Monitor Android APP (beta version) helped us to examine and analyze the properties of the Wi-Fi networks in the most efficient manner in the form of graphs. This Wi-Fi Analyzer and Surveyor from Manage Engine effectual supervise the signal strength, channel graph and channel interference of the available Wi-Fi networks at the Surrounding environment.