How To Write A Lab Report On Catalase

This experiment looked at how substrate concentration can affect enzyme activity. In this case the substrate was hydrogen peroxide and the enzyme was catalase. Pieces of meat providing the catalase were added to increasing concentrations of hydrogen peroxide in order to measure the effect of hydrogen peroxide concentrations on the enzyme’s activity. The variable measured was oxygen produced, as water would be too difficult to measure with basic equipment.

The human body is an incredible system that is capable of working a multitude of diverse functions. Without the help of the many different protein molecules, the human body would not be able to function properly. One major group of proteins called enzymes are mandatory for essential life. These proteins are constantly at work assembling molecules, metabolizing energy, and fighting off infections. An enzyme is a macromolecule that acts as a catalyst that speeds up a chemical reaction without being consumed by the reaction. Without these proteins, these reactions would take place too slowly to keep us alive. Essential parts in your body like vitamins and minerals cannot do any work without

This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide.

solutions using qualitative analysis techniques. 1 Silver (Ag + ) and lead (Pb 2+ ) created a solution of

A catalyst is a substance that increases the rate of reaction (Jim Clark, 2002) and in which is not effected once the reaction has occurred. Catalase are a form of Catalyst that are intended for converting the Hydrogen Peroxide which is produced by cells, into Water and Oxygen. Catalase are found in organic matter such as the liver and potato. The liver houses harmful toxins such as Hydrogen Peroxide, and is why the there is a high concentration of catalase. The potato has a reduced rate of catalase because of the unsophisticated functions of the organic matter. It was found that the liver contained a higher concentration of catalase compared to the low concentration in the potato. This was due to the function of the liver in the digestive

The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It was hypothesized that, until all active sites were bound, as the substrate concentration increased, the reaction rate would increase. The first experiment consisted of five different substrate concentrations, 0.8%, 0.4%, 0.2%, 0.1%, and 0% H2O2. The second experiment was completed using 0.8% substrate concentration and four different temperatures of enzymes ranging from cold to boiled. It was hypothesized that as the temperature increased, the reaction rate would increase. This would occur until the enzyme was denatured. The results from the two experiments show that the more substrate concentration,

An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of substrate on the enzyme’s reaction.

The chemical hydrogen peroxide(H₂O₂) is broken down by the enzyme catalase. Hydrogen peroxide is a byproduct formed in cellular reactions that, if not broken down, could inflict severe damage to the cell. Catalase is an enzyme that breaks down hydrogen peroxide in to water and oxygen. How efficient and strong the enzymes reaction to break down H₂O₂ determines largely on temperature and pH level. An enzyme only functions within a set pH and temperature range. Beyond that it becomes denatured, rendering it useless. The purpose of this lab is to determine at which temperature and pH level the enzyme catalase reacts best. Catalase in chicken and beef livers will be used to do the lab because enzymes still function after death as long as they are kept refrigerated at a low temperature.

Catalase are an enzyme that catalyses the reduction of hydrogen peroxide into H2O and O2. Catalase are a common enzyme that can be found in almost all living organisms such as potatoes. It is specifically found in the cells that are exposed to oxygen and can be in a plant or animal cell. [1]

As we study biomolecules, the subject of how do enzymes work comes up. The reasoning behind the catalase enzyme lab was to see how enzymes break down compounds. My objective being to see how the catalase enzyme will react to liver, potato and apple. We will also use a base or acid to see how these factors will affect the independent variable. My hypothesis is the liver and potato will react to the peroxide but the apple will not.

How do different environments affect the reactivity of catalase? If we add water to the to the hydrogen peroxide, then the reaction will not be as extreme as the control group because the hydrogen peroxide will be diluted. If we add HCl to the hydrogen peroxide, then the reaction will be stronger than the control group because the acids are working together. In this experiment the independent variable was the HCl and water added into the hydrogen peroxide (10 ml each).

A catalase is a molecule that speeds “up the rate of a reaction without themselves being used up or permanently altered” (Audesirk & Byers 2008). They do this by lowering the activation energy required for the reaction to begin. This allows a higher proportion of molecules to move fast enough to react when the molecules collide. Enzymes are biological catalysts. They are proteins that promote specific chemical reactions. In this lab experiment, the chemical equation is: 2H2O2 2H20 + O2. Catalase, an enzyme that destroys hydrogen peroxide (H2O2), is used here to facilitate the chemical reaction from the substrate (2H2O2) to the product (2H20 + O2). This demonstrates how catalase operates in our body to destroy harmful toxins. However, the reaction

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

reaction rate increases. If the temperature of an enzyme gets to high the reaction rate will slow

The purpose of this lab report is to investigate the effect of substrate concentration on enzyme activity as tested with the enzyme catalase and the substrate hydrogen peroxide at several concentrations to produce oxygen. It was assumed that an increase in hydrogen peroxide concentration would decrease the amount of time the paper circle with the enzyme catalase present on it, sowing an increase in enzyme activity. Therefore it can be hypothesised that there would be an effect on catalase activity from the increase in hydrogen peroxide concentration measured in time for the paper circle to ride to the top of the solution.