Intro to Micro Review Questions

3131 WordsFeb 4, 201113 Pages
Intro to Micro Review Questions Review questions are designed to help you study for exams. They are not intended to cover every question that may be on the exam. Questions on the exams may be over any of the material covered in class lectures and in your reading assignments. Review questions are arranged by topic, not by exam. Refer to the Lecture Schedule and your class notes for topics that will be covered on a specific exam. Introduction to Microbiology 1. What is microbiology? What types of organisms are studied by microbiologists? the study of very small living things a set of techniques for studying very small organisms What types of microorganisms are studied by microbiologists? 1. What are the three domains of…show more content…
7. Give an example of when each of the following would be a good choice – an agar slant, an agar deep, an agar plate, a broth tube. Agar in test tubes can be slanted while it is solidifying to make a larger surface area to inoculate. Agar deeps, which aren’t slanted, are usually used for “stabs” - inoculation by stabbing a needle with a sample of the microorganism deep into the agar. This can be useful in determining if the organism will grow - or grows best - in the presence or absence of oxygen, and also for seeing if the microorganism is motile. Agar plates allow the bacteria to be spread out more, so that isolated colonies can be studied. Broths are often used just to keep a culture growing, or with the addition of particular substances, to reveal differences in metabolism. 8. What is the purpose of nutrient media? What is the difference between a simple (or defined) medium and a complex medium? Nutrient media provide everything the organism needs to grow. Simple media are made by mixing up specific chemicals like salts, sugars, and amino acids. Because it is clear exactly what is in the medium, such a medium is often called a defined medium.

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