Khokana Outbreak Research Paper

An investigation was necessary to find the cause of the outbreak, and how to stop it from continuing to spread. The campus clinic was interested in testing the following nine patients: Sue, Jill, Anthony, Wanda, Maggie, Maria, Arnie, Marco, and Alvin. All of the students have similar symptoms and agreed to being tested except for Alvin.

These microorganisms are transmitted from poor hand hygiene from health care workers to patients as well as touching of contaminated equipment and environmental surfaces. Microorganisms are most commonly introduced to susceptible sites such as open wounds or other portals of entry by contaminated hands. Infection leads to adverse clinical outcomes and can directly threat patient recovery.

Hospital acquired infections (HAIs) affect over 1.7 million patients each year, causing almost 100,000 deaths annually in the United States alone (Johnson, 2010). According to the World Health Organization, HAIs are the most frequent adverse event in the healthcare industry. Fortunately, most of these infections can be prevented with one single intervention, proper hand hygiene (“The Evidence,” n.d.). Four out of five pathogens that cause illness are spread by direct contact. Proper hand hygiene eliminates these pathogens and helps to prevent cross-contamination and HAIs (Linton, 2015; “Hand Hygiene,” n.d.). Reduction of cross-contamination and HAIs improves patient outcomes, increases employee wellness, and lowers health care costs. Adherence to proper hand hygiene is the single most important safety measure in the health care setting. However, for many years compliance to proper hand hygiene in the healthcare industry has been dismally low. New and inventive measures must be implemented to increase compliance to proper hand hygiene and lower the rate of hospital-acquired infections.

| UNIT 4222- 264 THE PRINCIPLES OF INFECTION PREVENTION AND CONTROL | | | |

Unknown bacteria determined to be Alcaligenes faecalis because of its morphological, physiological and metabolic properties.

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

Germs and many other diseases spread primarily through airborne particles, skin to skin contact, and or touching objects such as door handles, hospital buttons, or by sharing patient possessions. Nurses and other health care clinicians are constantly in physical contact with many different patients, who all have varying illness’ and diseases themselves. Many may also carry a

faecalis has a low pathogenicity (scilo), it is a virulent, opportunistic pathogen to be reckoned with and is thought of as a super-bug. This is not only due to its ability to resist a variety of antibiotics, but also its ability to travel and employ biofilm formations. It can grow and adapt in many different environments. It can thrive in a wide range of temperatures and has disregard whether salt or oxygen are present, or whether the pH is basic or acidic. Its resilience on inanimate objects makes it a perfect candidate for transmission to occur within the hospital environment from hand to instrument, but it can also be spread via hand-to-hand contact and from food contamination (Public Health Agency of

The main idea of this experiment was to correctly identify the unknown bacteria, #3. Identification of unknown bacteria yields multiple benefits in many different areas in the research of microorganisms. In this experiment I performed many different test dealing with things such as the presence of enzymes, fermentation abilities and different chemical reactions. Observations made from the tests were then compared to a gram negative unknown chart in order to identify the bacteria. Based off of my results and the chart, I concluded the bacteria #3 was the bacteria Escherichia coli. E. coli is most commonly found in the intestines of warm blooded organisms. Most E. coli strands are non pathogenic however, there are strands

As the flowchart shows, a series of tests were conducted to identify the unknown bacterium #65. Microscopic observation of the gram stain indicated a gram-positive coccus bacterium. S. epidermidis was used as the gram-positive control while E. coli was used as the gram-negative control. This observation led to the elimination of all gram negative and rod-shaped genera: Enterobacter, Citrobacter, Klebsiella, Escherichia, Pseudomonas, Serratia, Alcaligenes, Neisseria, Proteus, Salmonella, Shigella, Erwinia, Veillonella, Flavobacterium, Bacillus, Arthrobacter, Lactobacillus, Listeria and Kurthia (2). By performing the catalase test, it was determined that the bacterium was catalase negative and it did not produce bubbles. M. luteus and E. faecalis were used as positive and negative controls, respectively.

Checking in to the hospital comes with a heavy price tag, and sometimes you get more than what you bargained for. As highly trained doctors, nurses, and staff make their way through the hospital, they carry with them microbial agents of disease. Although regarded as centers for treatment and prevention, hospitals are also known to harbor nosocomial, healthcare-associated, bacterial infections. These infections can be a result of overused or inappropriately used antibiotics and the breaching of infection containment policies by patients and staff. Though health-care-associated infections have been decreasing, one infection inciting nosocomial bacterial, Clostridium difficile has been rampant. It is important that inefficiencies in health-care be met with stringent efforts for prevention as they may lead to distressing financial, emotional, and medical repercussions.

Bacteria, single-cell microorganisms, are found all over the planet. Some can be beneficial to their enviroment, while others can be pathogenic. Enterococcus faecalis is a resilient bacterium that resides in the intestinal tract of humans and mammals. Due to their ability to colonize at a rapid rate, E. faecalis cause a multitude of infections. To determine an unknown bacteria, one gram stain and two biochemical tests, a catalase and RBC hemolysis test, were performed. The gram stain showed gram-positive cocci bacteria, which lead to the catalase test. The test for

Healthcare associated infections have an impact on patients - how? Can be prevented greatly with compliance to hand hygiene protocols (REF).

This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.