Introduction and Literature Review
Plants and some organisms use the process of photosynthesis to transform light energy into chemical energy and other constituents. This study focuses on the rate of photosynthesis. The photosynthesis rate is influenced by several factors such as the amount of chlorophyll (Benckiser, 2014). The rate of photosynthesis is determined by photosynthesis concentration (Campbell, 2006). The chlorophyll absorbs sunlight, which activates the photosynthesis process. Chlorophyll in green plants reflects green light. Furthermore, green plants contain carotene and xanthophyll (Benckiser, 2014). Though, the effects of both chlorophyll a and b outshine the effects of xanthophyll and carotene (Campbell, 2006). The technique of chromatography is suitable method to analyze and compare pigments of photosynthesis. This research used the method of chromatography to compare and analyze pigments of photosynthesis.
The regulation of the amount of the light that falls on the plant is another technique of measuring rate of photosynthesis (Campbell, 2006). The influence of light on the rate of photosynthesis was determined by controlling the distance between the plant and the light source.
Studies show that the rate of photosynthesis is directly affected by the light color in which the plant is exposed to (Iancu and Chilom, 2015). On the other hand, the plants that are nearer to the light source carry out photosynthesis faster as compared to plants that are at a
Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.
There are two main types of chlorophyll, chlorophyll a which absorbs wavelengths of 430nm (blue) and 662 (red) and is the main photosynthetic pigment, and chlorophyll b, which doesn’t directly participate in the photosynthetic process, but is capable of donating its energy to chlorophyll a
The data in the graph and table does not support the hypothesis that if spinach leaves are given red and blue light, then the blue light leaves’ photosynthetic rate will be higher than the red plant’s because blue has a shorter wavelength of light and a higher absorbance rate and therefore has a higher level of energy in order to help the plant photosynthesize most effectively (Braddock). As shown by table 1’s photosynthetic rates, the blue rate of photosynthesis was lower than the red with -0.00559 being 0.00106 points lower than that of the red photosynthetic rates. This shows that from minutes 0-22, the red light’s photosynthetic rate was (while still lower than the rate of cellular respiration) higher than the blue light’s rate of
Photosynthesis is the conversion of light energy to chemical energy into sugars. It is the process in plants that uses carbon dioxide, water, and sunlight from its surroundings and releases oxygen as a byproduct (6H2O+6CO2+light energy -> C6H12O6+6O2). Photosynthesis is required for plants because they are autotrophs, organisms that make their own food. Plants require a specific environment that is ideal to them to be able to carry out the process. Environmental conditions can either increase or decrease the rate of photosynthesis. Things like colors of light, pH, and temperature can all affect the rate of photosynthesis in plants.
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.
This lab was conducted to explore the light energy, pigments and the rate of photosynthesis in magnolia leaves. In experiment one a magnolia leaf was used to see the separation of primary and accessory plant pigments using a process called paper chromatography. The importance of this process was to discover which pigment had the highest band along a piece of filter paper and identify various plant pigments in a magnolia leaf such as xanthophyll, chlorophyll a, chlorophyll b, and carotenoids that aid magnolia leaves during photosynthesis. Based on the conducted experiment, it can be concluded that chlorophyll a was the pigment that showed the highest band on the piece filter paper which means that chlorophyll a is the primary pigment in photosynthesis
Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water, PO4 buffer, and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of photosynthesis by using a spectrophotometer, measuring the greatest change in absorbance. From this experiment, two data charts and four graphs were obtained. The hypothesis was set from graphs obtained in this lab, and the optimal reaction
Virtual Lab #5- “Which Colors of the Light Spectrum are Most Important for Plant Growth?
In my research paper, I will attempt to determine how the perception of light in phytochromes plays a role in the development of plants. Specifically, I will look at how phytochromes play a role in the growth and development of Arabidopsis thaliana. The paper will also look at how light perception plays a role in phototropism and the immune systems of a plant. Finally, my paper will explore how changing light conditions impact perception in phytochromes.
Based on your data, draw a conclusion regarding how light intensity affects the rate of photosynthesis.
The purpose of this lab is to determine the relationship between photosynthesis and cellular respiration.The effect of Light Intensity experiment will show the rate of photosynthesis based on the amount of light from the light bulb, temperature, and direction and distance of the light, these variables determine the absorbance. In the effect of Light Wavelength experiment, photosynthesis is affected by different light colors. Photosynthesis in this experiment is more successful with certain colors due to different pigments in chloroplasts only absorbs certain wavelengths. The rate of photosynthesis will be estimating oxygen production in spinach leaf using floating leaf disk procedure. The more floating disks, the more oxygen being produces
What is the relationship between an increase in light intensity and photosynthetic rate in tomato leaves?
The authors are trying to determine how elevated Tsoil and elevated CO2 will affect the physiological responses of shade-tolerant species in light conditions. Also, how elevated CO2 and high light treatment affect physiological traits of shade-tolerant plants.
However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.