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Liver Cancer Case Study

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Description of Procedures This proposal is to investigate the role of tumor immune microenvironment changes in local recurrence and metastatic spread of liver cancer after catheter-based therapies using an orthotopic HCC rat model. We will use 250-350 buffalo rats (male and female) between 5 to 7 weeks of age. A subgroup of animals will be used to fine-tune the effective dose of treatment agents and advanced imaging protocols proposed. Survival surgery procedures will be performed using aseptic technique in an approved surgery area, with a dedicate space for surgical support, animal preparation, operating area, and post-operative recovery. For all studies, the liver of isoflurane-anesthetized buffalo rats will be exposed via…show more content…
Justifications The vasculature of the rats, opposed to mice, is of adequate size that permits intra-arterial delivery of therapies, in addition to intratumoral and intravenous delivery. The Buffalo rat, orthotopic HCC model, has been used extensively in interventional radiology preclinical work aimed at understanding and determining treatment efficacy of different agents. The specific characteristics of the liver tumors obtained with this method, such as size, perfusion, level of baseline necrosis, cannot be replaced with other species and provide a unique and the best model to test the role of the treatments of interest with a meaningful clinical relevance. Minimization of Pain and Distress For all surgical procedures and imaging protocols, each rat will be anesthetized with isoflurane using an anesthesia machine, including inhalant (isoflurane) and oxygen supply. Animals will be placed in a clear induction chamber and then transfer to a sterile surgical area for deep anesthesia. Sterile ophthalmic lubricant will be applied to the eyes once animals are anesthetized. Depth of anesthesia will be monitored by respiratory rate and absence of withdrawal reflex to footpad pinch. To maintain the corporal temperature, animals will be wrapped with towel and a surgical drape. Surgical sites will be
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