Luciferase Assay Paper

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Luciferase assay. N2a cells were seeded into 24-well plates and transfected with 0.2 g of 8XTOPFLASH reporter and 0.05 g of pRL-TK, 0.4 g WT-DISC1 or DN-DISC1 and 0.4 g mouse DLX2 [50] using polyethylenimine. 24 hours after transfection, TCF reporter activity was measured using the Dual-Luciferase Assay System (Promega). Behavioral tests The pregnant female mice were fed with Dox containing food (Bio-Serv, 200 mg/kg) from the beginning of pregnancy on embryonic day 0(E0). The Dox food was removed right after the littermates were delivered on postnatal day 0 (P0). The single transgenic Nes-rtTA littermate mice from the same pregnant mother were used as control. The control and Nes-DN-DISC1 littermates (6-15 mice each group) were tested …show more content…

Mice were placed in the center of the maze and, allowed to freely move into the four arms of the maze for 5 minutes. The mice were videotaped and the times spent in open and closed arms were scored. Novelty Suppressed Feeding Novelty Suppressed Feeding is a test for chronic depression. Mice were weighed and food deprived from their cage 18 hours before the test. Each mouse was placed in the corner of a chamber that is 18” by 24” covered in bedding. At the center of the chamber was a small amount of food placed on a white whatman filter paper that is 5 cm in diameter. The center area was brightly lit. The animals were allowed to move around freely for the next 6 minutes, and the time the animal took to start eating the food were measured, as well as the total time spent eating. Fear Conditioning Test In the training session, each mouse was adapted in the fear conditioning instrument for 5 min. After the habituation, the mouse in the testing chamber will receive a 2-sec, 0.55-mA foot shock every 80-sec [52]. The mouse was removed immediately after the third shock. During the contextual testing, the freezing behavior of each mouse was recorded in the testing chamber for two groups. Grooming Test Grooming test was used to measure the repetitive behaviors[53]. The face of each mouse was misted with a spray of distilled water and mouse was placed into an empty cage with no bedding. The animals were videotaped for 15 min.

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