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Persistent Fatigue Lab Report

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1. INTRODUCTION

1.1 Background Information
It was only after I found myself experiencing symptoms of persistent fatigue did I decide to go see a doctor. Having provided details about my fatigue, hair loss, dizziness and loss of focus, the doctor decided to order a blood test to determine the cause of these symptoms. After performing a complete blood count test (CBC), which is often used to measure the number of blood cells in a sample of blood (including red blood cells and hemoglobin, the iron-containing molecule in red blood cells that carries oxygen), results were reviewed and I was diagnosed with anemia, which was caused by an iron deficiency. This means that my stores of iron were too low to support red blood cell production.
Beef, …show more content…

This allows iron(II) to be oxidized to iron(III), hence the two reactants are permanganate (MnO4-) and Iron (Fe2+), and the products will be manganese (Mn2+) and iron (Fe3+). The two half-equations for the reaction can be seen below:
Fe2+  Fe3+ + e-
MnO4- + 8H+ +5e-  Mn2+ + 4H2O
These combine and the overall redox reaction is described by the following chemical equation:

MnO4- + 8H+ + 5Fe2+  Mn2+ + 4H2O + 5Fe3+

Throughout this investigation compounds will be carefully measured so that their concentrations and volumes can be determined to a high degree of accuracy. This way I will titrate an iron solution with potassium permanganate until the endpoint —the point at which the solute at the flask (Fe2+) has reacted completely and no further titrant (MnO4-) should be added. This will be indicated by a change in colour, with the solution showing the first persisting faint pink colour.
Now some necessary calculations have to be made so that the experiment can proceed. Using the known concentration and volume of used MnO4- and the knowledge of the stoichiometry of the reaction, we can estimate the iron content after a multiplication of the moles of Fe2+ and its molar mass (55.845 g/mol).
Materials & Apparatus · 250 cm3 conical flask
· 250 cm3 volumetric flask
· 5 Fero-Gradumet iron …show more content…

Additionally, concentrated sulfuric acid is very corrosive to eyes and skin.
Safety googles, gloves and an apron should be worn for this experiment. Preparation of iron (II) solution: The mass of 5 iron tablets is measured by electronic balance. Using a mortar and pestle, the weighted tablets are crushed manually and turned into homogeneous powder. Ground material is transferred to a 250 cm3 glass beaker with 200cm3 of deionized water in it. The solution is stirred with glass rod. This solution is transferred to a 250cm3 conical flask that had been previously washed with deionized water. The solution is made up to the graduation mark with 50cm3 of 2.0M H2SO4. The conical flask is stoppered and inverted 10 times to ensure sufficient mixing. Water is sprayed around the glass surface to minimize chances of unwanted Fe residues.
Determining the concentration of iron Prior to the titration, all glassware is to be washed with deionised water. Burette and pippete respectively are rinsed with the solution they will contain to minimise error. Using a funnel, the burette is filled up with 0.1 M Potassium permanganate. Ensure that the part below the tap is filled prior to adjusting to

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