Photosynthesis Lab Report

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

The purpose of this lab was to see which level of light (measured in lux) made Spinacia oleracea (Spinach) leaf disks float the fastest. Our hypothesis was that an increase in light intensity will decrease the time it takes Spinacia oleracea disks to float. If light intensity is increased, then the time it takes Spinacia oleracea disks to float will be decreased. The mean for the no light (0 Lux) sample and the low light (4 x100 Lux) sample was 1200 seconds with no standard deviation because none of the disks in these two samples floated. The mean and standard deviation for the medium light (110 x100 Lux) was 902 seconds +- 84 seconds. The mean of the high light sample (410 x 100 Lux) was 692 seconds with no standard deviation because only two Spinacia oleracea disks floated so there was no need to measure the variability of the data. The final results indicated that the highest light intensity led to the quickest rise of Spinacia oleracea disks, supporting our hypothesis.

Introduction: Photosynthesis can be defined as a solar powered process that removes atmospheric carbon dioxide and transforms it into oxygen and carbohydrates (Harris-Haller 2014). Photosynthesis can be considered to be the most important biochemical process on Earth because it helps plants to grow its roots, leaves, and fruits, and plants serve as autotrophs which are crucial to the food chain on earth. Several factors determine the process of photosynthesis. Light is one these factors and is the main subject of this experiment. The intensity of light is a property of light that is important for photosynthesis to occur. Brighter light causes more light to touch the surface of the plant which increases the rate of photosynthesis (Speer 1997). This is why there is a tendency of higher rates of photosynthesis in climates with a lot of sunlight than areas that primarily do not get as much sunlight. Light wavelength is also a property of

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the

The hypothesis that red light would produce more dissolved oxygen was not supported by the results of the experiment. Without having more accurate data it is impossible to say which color light induced its plant to produce the most oxygen. The reason for not having more accurate data is because of the way of testing the dissolved oxygen. By testing with the dissolved oxygen tabs the data could not have been very specific and specific data is important for a good experiment. The way of getting much more accurate data is by testing with the Winkler's method. This way of testing for dissolved oxygen amounts would have not been possible to execute because of the amount of water and the size of tanks used. To test with the Winkler’s method it is necessary to test 200 mL at a time. If this way of testing for

Abstract: An experiment was carried out to determine how certain factors such as light intensity and availability of carbon dioxide, affected the rate of photosynthesis. The rate of photosynthesis was measure by the amount of oxygen produce (cm3/min).

This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.

Chloroplast Activity in Cells Questions & Hypothesis In wet lab two, our group decided to use the cells of broccoli to perform our experiments. Part one of our experiment was meant to focus on the presence of chloroplasts in solutions of isolation buffer and DCIP. Our group was attempting to find which cell fractionate solution contained the highest amount of broccoli chloroplasts after three separate suspensions were made (P1, P2, S2). The presence of chloroplasts in the solution is directly related to a decrease in measured absorption of 600 nm wavelengths by a spectrometer (Leicht and McAllister 85).

Results found the necessity for light and CO2 in photosynthesis. As shown in Figure 1, the light + CO2 treatment had approximately 55% of disks float. The mesophyll layer of a leaf has many spaces filled with gas, causing floatation. However, all gases were removed from the Beta vulgaris disks in this experiment and replaced with water and dissolved sodium bicarbonate respectively. The sodium bicarbonate releases CO2, allowing photosynthesis to occur. Exposure to light also helps the Beta vulgaris photosynthesize and produce oxygen, therefore causing floatation. Light enters the chloroplasts of a plant and becomes stored as chemical energy in organic compounds, which are produced from CO2 (Mudie, K. and Brotherton, J. 2004). This supports the idea that light and CO2 together drive photosynthesis to

The control in this experiment was the white light because it has all the colors available to the plant.

The effect of chloroplasts on the growth of a plant Purpose: The purpose of this experiment is to determine how different factors affect the rate of photosynthesis. Hypothesis: If the amount of chloroplast relates to photosynthesis then it is predicted that the more chloroplast present in the leaf the more oxygen bubbles produced because the function of chloroplasts is to capture energy from sunlight and use it to conduct photosynthesis, without this organelle photosynthesis would be impossible diminishing rate of photosynthesis.

The experiment CO2 deceased and increased in the results. For the experiment our hypothesis was CO2 deceases when you test the light that means that photosynthesis is increasing. Photosynthesis will not take place without light. The hypothesis was predicted was correct because the results from the data CO2 has a positive and negative slope. During the experiment fake leaves was tested with light which had a result with CO2 release.

This lab allowed the experimenters to understand the effect of light on the producer’s photosynthesis. Once understanding how the light dependent reactions of photosynthesis worked, the experimenters can make predictions on how the light will affect the photosynthesis. For example, a low amount of light will affect the light dependent reactions of photosynthesis because light is essential for those reactions. The question the experimenters asked was, “Does having more or less exposure to light cause a faster rate of photosynthesis?”

However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.