Plants require the conduction of photosynthesis in the ability to develop and grow. Photosynthesis occurs within the chloroplasts of cell. The process of photosynthesis requires the production of oxygen and the flow of electrons to conduct itself. Prior to the 1930’s experiment conducted by Robert Hill, it was unknown as to whether these things could occur without the presence of carbon dioxide. In his 1930’s experiments Robert Hill was the first to measure the oxygen that evolved by partially purified isolated chloroplasts (Pennazio 2008, pp.445) and discovered that electron flow and oxygen production continued without the presence of carbon dioxide as long as there is a presence of an electron acceptor (Deakin University 2016, pp.49). Similarly in this experiment the aim was to determine whether chloroplasts can continue to carry out oxygen production and electron flow in the absence of carbon dioxide. In contrast to the way that Hill’s experiment used a dye that lost colour when there was the gain of electrons, the dye used in this experiment DCPIP, which was used instead of NADP+, losses its blue colour when it is reduced from its oxidised state (Deakin University 2016, pp.50). Based on the findings by Robert Hill it is hypothesised that in the use of DCPIP as a coloured indicator for the conductance of photosynthetic reactivity of the chloroplasts will allow a continuance of the production of oxygen and electron flow, even in the absence of carbon dioxide, and that
Light intensity is a key component in photosynthesis, amongst carbon dioxide and water to sustain a suitable rate of photosynthesis. Chlorophyll absorbs the light, causing photoexcitation and the formation of NADPH and ATP with production of O2 as a by-product. The Calvin Cycle takes the NADPH and ATP to reduce CO2 into sugars (CH2O), and return NADP+ and ADP + Pi to the light reactions. The process will then repeat. (Reece, et al, 2015)
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
-Measuring the pH of a solution (such as in the lab we had) could also help determine the rate of photosynthesis. You would need a much more specific pH meter, but generally, if pH goes down, the level of CO2 is higher, meaning more cellular respiration. Higher pH means there’s less CO2, so more photosynthesis.
The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the
Hello, my name is Audrey and welcome to my presentation on the chemistry of photosynthesis and cellular respiration.
In this lab, you will be experimenting with how different variables affect the rate of photosynthesis.
Photosynthesis occurs in the palisade mesophyll of plant leaves. During photosynthesis, carbon dioxide is broken up into 6 carbon for the sugar molecule and 12 oxygen. In this lab, I determined the rate of photosynthesis in spinach
If you expose a photosynthesizing plant to water that contains both radioactive H and radioactive O, in which of the products of photosynthesis will the radioactive H and O show up?
photosynthesis happens in two stages: light reaction and carbon fixation also known as calvin cycle.light reaction TAKES PLACE IN THYLAKOID USE light energy to produces atp and nadph whereas, calvin cycle takes place instroma uses energy derived from light dependent reaction to make GA3P from CO2 ( Bio166 lab execise manual, 2015). the purpose of this experiment was to separate plant
First, the three reactants of photosynthesis are Carbon Dioxide, Water, and Sunlight. These are the three things that a plant needs to survive. Carbon Dioxide is what plants breath in, we breath out carbon dioxide and they release oxygen, so it is a exchange sort of. Water is what keeps the plants hydrated so they can function. Also plants need sunlight because they turn light into their food, so it is important for the process of photosynthesis.
For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.
Photosynthesis is a vital process that autotrophs use to transfer light energy into chemical energy. Photosynthesis ultimately produces O2 and glucose. It, like many other biological processes, can be affected by environmental variables. The variable that we altered in the following experiment are intensity, light wavelengths, and pigment types. In order to do this, we conducted three experiments. In the first experiment, we examined the effect of light intensity by placing vials with chloroplasts with DPIP at different light distances in which the results varied. Initially, 30cm away was the most effective for photosynthesis. Then 24cm appeared to be the most effective. Followed by 49cm at minutes 25 and 30. In the second experiment, we
Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special way of using the ATP they produce. Photosynthesis is a process where plants harness the sunlight they receive and they produce carbohydrates, as well as oxygen for living things and other plants. Now the sunlight ultimately powers the process of
Photosynthesis has a two-stage performance before plants produce the two products they are known to produce. These stages are Photosystem I and II. Photosystem II is dependant on light reactions for energy which causes the electrons to be react and be transferred to Photosystem II. The electrons are transported through the Photosystem II electron transport system, however some energy is used to drive ATP synthesis. Meanwhile, light is being absorbed by the Photosystem I, which causes the electrons to react. This process sends the electrons to the Photosystem I transport system where some energy is released as electrons travel through the electron transport system and is captured as NADPH. When this process is completed oxygen is released from the plant and glucose has been
Light is needed in the first stage which is called the light dependent stage. Light is absorbed in the leaf, also called the lamina, because it has a greater surface area and has more chloroplast where you find chlorophyll which absorbs the light. This raw factor is very limiting to this process, without it photosynthesis will not happen, even if you increase the amount of carbon dioxide and temperature it still won’t occur. This article is relevant to my investigation and has good