Pseudomonas Aeruginosa Essay

Pseudomonas aeruginosa Pseudomonas aeruginosa is a versatile gram negative bacterium that grows in soil, marshes, and coastal marine habitats, as well as on plant and animal tissues. People with cystic fibrosis, burn victims, individuals with cancer, and persons infected with HIV are particularly at risk of disease resulting from Pseudomonas aeruginosa. Unlike many environmental bacteria, Pseudomonas aeruginosa has a remarkable capacity to cause disease in susceptible hosts. It

Pseudomonas aeruginosa is an opportunistic human pathogen and one of the leading causes of nosocomial infections worldwide [1]and causes a variety of infections especially in immunocompromised patients such as burned patients. Infections caused by P. aeruginosa are associated with significant morbidity and mortality [2, 3]. The organism exhibits a high level of intrinsic resistance and only a limited number of antimicrobial agents are active against it [4]. In addition, P. aeruginosa has acquired

The aim of this project is to develop two bioanalytical sensing platforms as a means to study phenomena in organized 3D cellular microenvironments that appropriately model key aspects of biological habitats. Three important questions, listed below, will be addressed during this project, which we discuss in detail in Sections 3.1-3.3. (1) Can we determine concentrations of cellular biogenic species (NO, H2O2) in organized microcommunities using T-CUAs? (2) Can we monitor proximity-dependent cellular

period. The results were compared against standard curve developed using a concentration gradient of Na2So4 with BaCl2 (De Zoysa et al., 2007). 3.15. FTIR of purified polysaccharide FTIR (Fourier Transform Infrared Spectroscopy) spectra of purified PS fractions were determined using FT-IR spectrophotometer model 5700 (M/S Thermo electron Corporation, USA). Polysaccharide powder (2–3 mg) was mixed with KBr and pressed into a disk. The whole IR spectrum was analyzed with a scan range of 4000–400 cm-1

Colistin is a type of polymyxin, which are cyclic lipopeptides typically with the structure below. Each type of polymyxin is characterized by distinct amino acid residues present at positions 6 and 7. In colistin, also known as polymyxin E, a D-leucine is present at position 6 while position 7 contains a leucine residue. Colistin contains 13 stereocenters.11 Colistin is produced in Bacilius colistinus Koyama via fermentation.12 These cells can be grown in a 1% glucose medium, and then harvested

Healthcare-associated pneumonia (HAP) is a common complication of being hospitalized, especially in the elderly. On average, HAP increases the length of stay (LOS) to seven to nine days, adds $40,000 to $65,000 in cost of care, and is a big contributor to morbidity and mortality risks (Quinn, et al., 2014). The World Health Organization reports that 1.4 million people die annually related to pneumonia infections (Driver, 2012). A comparison of community-acquired pneumonia (CAP) and HAP indicates

Introduction This article explores the exciting effects of antibiotic resistance and how we can utilize this knowledge to create better treatments against bacterial infections. It is necessary to know how resistant a strain of bacteria is to a specific antibiotic so that treatment can be changed to better treat the patient. In this study, 200 strains of the Gram-negative bacteria, Klebsiella pneumoniae (K. pneumoniae), were tested for resistance against thirteen different antibiotics by the method

1.6.1 Classification of β-lactamases Two major schemes for classification of β-lactamases are currently in use. The molecular classification of β-lactamases is based on the amino acid sequence homology dividing them into four classes A through D. The enzymes belonging to class A, C and D utilize serine residue for substrate hydrolysis while class B enzymes (metallo-β-lactamases) utilize divalent zinc ions for β-lactam hydrolysis [Ambler R. P. et al, 1991]. The functional classification is based

A portion of (2–3 mg) from the partially purified polysaccharide powder was mixed with KBr then pressed into a disk. The whole infrared spectrum was analyzed at a scan range of 4000–400 cm-1. Thirty scans were taken with 4 cm-1 resolution. CO2 and H2O corrections were incorporated. Reproducibility of the normalized spectra was ±2%. (Shanthi et al., 2014). 3.16. Testing isolates probiotic properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann

I. Description of P. aeruginosa Pseudomonas aeruginosa is characterized by aerobic, gram negative, motile, non-spore forming bacilli (rods).1 A trait that differentiates P. aeruginosa from other gram-negative bacteria is the fact that it produces indophenol oxidase, which is an enzyme that renders them positive in an oxidase test.1 The type of flagella this organism is known to possess is called a polar flagella, meaning a single flagellum at one pole of the rod.1 This means the motility of the organism