Pseudomonas aeruginosa Pseudomonas aeruginosa is a versatile gram negative bacterium that grows in soil, marshes, and coastal marine habitats, as well as on plant and animal tissues. People with cystic fibrosis, burn victims, individuals with cancer, and persons infected with HIV are particularly at risk of disease resulting from Pseudomonas aeruginosa. Unlike many environmental bacteria, Pseudomonas aeruginosa has a remarkable capacity to cause disease in susceptible hosts. It
period. The results were compared against standard curve developed using a concentration gradient of Na2So4 with BaCl2 (De Zoysa et al., 2007). 3.15. FTIR of purified polysaccharide FTIR (Fourier Transform Infrared Spectroscopy) spectra of purified PS fractions were determined using FT-IR spectrophotometer model 5700 (M/S Thermo electron Corporation, USA). Polysaccharide powder (2–3 mg) was mixed with KBr and pressed into a disk. The whole IR spectrum was analyzed with a scan range of 4000–400 cm-1
The aim of this project is to develop two bioanalytical sensing platforms as a means to study phenomena in organized 3D cellular microenvironments that appropriately model key aspects of biological habitats. Three important questions, listed below, will be addressed during this project, which we discuss in detail in Sections 3.1-3.3. (1) Can we determine concentrations of cellular biogenic species (NO, H2O2) in organized microcommunities using T-CUAs? (2) Can we monitor proximity-dependent cellular
Introduction This article explores the exciting effects of antibiotic resistance and how we can utilize this knowledge to create better treatments against bacterial infections. It is necessary to know how resistant a strain of bacteria is to a specific antibiotic so that treatment can be changed to better treat the patient. In this study, 200 strains of the Gram-negative bacteria, Klebsiella pneumoniae (K. pneumoniae), were tested for resistance against thirteen different antibiotics by the method
1.6.1 Classification of β-lactamases Two major schemes for classification of β-lactamases are currently in use. The molecular classification of β-lactamases is based on the amino acid sequence homology dividing them into four classes A through D. The enzymes belonging to class A, C and D utilize serine residue for substrate hydrolysis while class B enzymes (metallo-β-lactamases) utilize divalent zinc ions for β-lactam hydrolysis [Ambler R. P. et al, 1991]. The functional classification is based
A portion of (2–3 mg) from the partially purified polysaccharide powder was mixed with KBr then pressed into a disk. The whole infrared spectrum was analyzed at a scan range of 4000–400 cm-1. Thirty scans were taken with 4 cm-1 resolution. CO2 and H2O corrections were incorporated. Reproducibility of the normalized spectra was ±2%. (Shanthi et al., 2014). 3.16. Testing isolates probiotic properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann
The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii, regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods, optimum conditions and the
I. Description of P. aeruginosa Pseudomonas aeruginosa is characterized by aerobic, gram negative, motile, non-spore forming bacilli (rods).1 A trait that differentiates P. aeruginosa from other gram-negative bacteria is the fact that it produces indophenol oxidase, which is an enzyme that renders them positive in an oxidase test.1 The type of flagella this organism is known to possess is called a polar flagella, meaning a single flagellum at one pole of the rod.1 This means the motility of the organism
between Lec A and that of glycosphingolipid Gb3, termed as Lipid Zipper triggers the bending of plasma membrane in bacterium Pseudomonas aeruginosa. In vitro, cellulo experiments and theoretical model confirms the study that lipid zipper is enough for the triggering and bending of plasma membrane and the absence of Lec A or depletion of Gb3 reduces the uptake of P. aeruginosa. Thus our study provides new targets for drugs research. Introduction Lipid rafts involves in the signal transduction across
Pseudomonas aeruginosa is a key opportunistic pathogen characterized by high-level antibiotic resistance and biofilm formation (1).Biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix adherent to an inert or living surface. Biofilmproducing organisms are more antimicrobial resistant than organisms without biofilm. In some extreme cases, the concentrations of antimicrobials required to kill biofilm positive organisms can be three- to four-fold higher than