Major Characteristics Of Pseudomonas Aeruginosa

There are many differents ways to identify a bacterial unknown and many different situations where identification would be beneficial. One way to identify bacterial unknowns is to perform biochemical tests. In this experiment multiple biochemical tests were done, by performing these tests on the bacterial unknown received the two different bacteria were then identified. The citrate test is done to test the ability of organisms to use citrate as a carbon source. This test uses Simmons citrate agar, the agar contains sodium citrate as the only carbon source and has bromothymol blue as the pH indicator. The organisms that use citrate as a carbon source use the enzyme to transport the citrate into the cell. The cells converts ammonium dihydrogen

Unlike many environmental bacteria, Pseudomonas aeruginosa has a remarkable capacity to cause disease in susceptible hosts. It has the ability to adapt to and thrive in many ecological niches, from water and soil to plant and animal tissues. The bacterium is capable of utilizing a wide range of organic compounds as food sources, thus giving it an exceptional ability to

See Table 1 and Flow Chart 1 for results of Bacteria # 1 and Table 2 and Flow Chart 2 for results of Bacteria # 2.

P. aeruginosa is a ubiquitous Gram-negative bacterium that thrives in moist environmental reservoirs such as in the soil, water and plants8, 9. P. aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals, lending to its association with life-threatening illnesses10. In addition to pulmonary infections in CF patients, P. aeruginosa is frequently found in nosocomial infections11. As such, P. aeruginosa is recognized for its medical importance in clinical infections.

In this experiment, an unknown bacterium was given to each individual student. The main purpose of this lab was to identify the given unknown bacteria going through a series of biochemical tests as one of the gram negative bacteria among six different Gram negative bacteria Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Salmonella typhimurium. At the very beginning, streaking method; T-streak technique was used to isolate the pure colonies. For the morphological identification of unknown bacteria, Gram Stain Method was done. Biochemical tests that were conducted for the experiment

The pathogen in the NPR story was called Klebsiella pneumoniae, which is a Gram-negative coccobacilli, relatively small, (0.5-0.8/ 1-2um), does not form spores, and is easily fixed. ("Klebsiella Pneumoniae Morphology") This pathogen is arranged either singly or in pairs and clusters, and can be cultivated on ordinary media. On agar media, the bacteria forms grayish-white colonies which

“While motility is commonplace among the prokaryotes, it is important to note the variety of structures responsible for motility. These structures vary depending not only on the organism in question, but also on the particular environment” (Bardy, Ng, & Jarrell, 2003). “Study of the bacterial flagellum has provided insights into many aspects of prokaryotic cellular activities including genetics and regulation, physiology, environmental sensing, protein secretion and assembly of complex structures” (Bardy, Ng, & Jarrell, 2003). “Continued study of all prokaryotic motility structures will provide knowledge that is likely to reach far beyond the topic of motility and pathogenicity” (Bardy, Ng,

The purpose of this laboratory exercise was to perform tests necessary to be able to distinguish one microorganism from 10 others. Using a series of biochemical tests and characteristics, unknown #22 was concluded to be Pseudomonas aeruginosa. A dichotomous key was mapped out and used during this process. Using this provided guidance as well as organization as to what the result may be.

Proteus vulgaris also produces urease which can increase the chances of pyelonephritis. It does this by hydrolyzing urea to ammonia, which in turn, makes urine more basic. The basic environment allows the bacteria to survive and flourish (NCBI, 2008). Another important virulence factor includes the microbe’s motility. It moves by a mechanism called swarming, which is defined as a rapid surface movement by use of rotating flagella. The swarming effect allows the bacteria to move about the host in great numbers (NCBI, 2010). The combination of fimbriae, urease production and swarming favors the production of urinary tract infections.

On December 27,2011 Beaumont Health System noted an outbreak of Pseudomonas aeruginosa. This outbreak was then observed and studied to find the potential cause. The patients that developed this bacteria were observed and had all undergone cardiovascular surgery and also were from the same ICU unit. There was no evidence of this outbreak in any other area of the hospital. A common factor

Pseudomonas aeruginosa is a key opportunistic pathogen characterized by high-level antibiotic resistance and biofilm formation (1).Biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix adherent to an inert or living surface. Biofilmproducing organisms are more antimicrobial resistant than organisms without biofilm. In some extreme cases, the concentrations of antimicrobials required to kill biofilm positive organisms can be three- to four-fold higher than for biofilm negative bacteria, depending on the species and drug combination (2). Biofilms have great importance for public health as they are the main cause of nosocomial infections, especially implant-based and chronic infections (3). Antibiotic resistance in biofilms is due to a combination of many factors that act together to result in a level of resistance that is much higher than that of planktonic bacteria (4,5).

Background: Cystic fibrosis facilitated chronic lung infections caused by Pseudomonas aeruginosa have caused many great tribulations within the healthcare field. As a highly opportunistic bacteria, many treatments fail to fully eradicate the biofilms layers within the thick mucosal membrane, which lines the lungs of CF patients.2 The median life expectancy for CF patients is around 40 years, however those who acquire P. aeruginosa within

Characteristics of F. tularensis’ next taxonomic rank, as part of the Phylum Proteobacteria, characterize it as a Gram-negative bacterium. When gram-stained, this microorganism appears as a reddish color under the microscope. This is mainly because Gram-negative bacteria have an inner

The bacteria that was contained within Unknown tube #12 is believed to be Pseudomonas aeruginosa, Figure 1. The bacteria tested to be Gram Stain negative, producing a pink, red color retained from the staining process. When the species of bacteria was plated on nutrient media, the cells produced an irregular and spreading configuration as shown in Figure 2. This same plating test provided the margins and elevation, lobate and hilly, respectively. The specimen was stabbed in a Fluid Thioglycollate Medium (FTM) tube using an inoculated loop of the bacteria. The results of this experimentation indicate the type of oxygen requirement of the bacteria. The test found the bacteria to be aerobic as colonies of the bacteria began to form along the top of the FTM tube (Manual 2017).

Microorganisms, or microbes, and single-celled organisms exist within all niche ecosystems found without the world. They have been found to cluster together, living in highly complex assemblages instead of existing on their own, interacting on many different levels with symbiotic relationships being formed. Biofilm is one such example of this assemblage behaviour. Through synergistic symbiotic relations, microbes congregate to form a matrix-enclosed microbial accretion which can adhere to either a biological or nonbiological surface (Hall-Stoodley, Costerton, & Stoodley, 2004). Biofilms can be found throughout a diverse range of different environments and first appear in the fossil record very early at approximately 3.25 billion years ago, being formed from highly varied ranges of both prokaryotic archaea and bacteria (Hall-Stoodley, Costerton, & Stoodley, 2004). Even though there are many different types, the main focuses are on both infectious biofilms and built environment biofilms, as these are the most directly impacting. Multiple processes are present such as syntrophy, signalling and coordination with structure and emergent order; make these assemblages incredibly structurally tough and dynamic. The stages of development, from motility to fixing a position, expansion and finally dispersal, display numerous different signalling and regulation pathways, making them highly complex and one of the main factors that sparked recent research.