Taste And Smell Lab

22. Pushing the stopper to make sure it is firmly attached to the mouth of the syringe, press the plunger inward.

12.Stir then pour 2.5ml of the enzyme mixture into one of the test tubes not allowing any water from the saucepan into the test tube.

After carefully taking them out, observe each test tubes color and then fill Table 2 with the results.

* By using the dropper and measuring cylinder, 3ml of 1% lipase was added into the test tube

2. Four unknown samples were included in the lab kit: flax seed meal, potato starch, egg whites, and dried milk. Using the results of the biochemical testing, determine which number corresponds to the correct unknown. (8 points)

8. Which meal had the least percentage of markers in the total colon at 24 hours?

2. We measured 1 mL of turnip peroxidase (the enzyme) and 3 mL of neutral buffer (pH corresponding to the test tube number i.e. pH 5 in test tube 5) with a syringe and disposed it into tubes 3, 5, 6, 7, 8, and 10

1. (10 points) Write out your pre-lab prediction, including what evidence or previous knowledge you have to support that prediction.

The mixture might have contained more or less than 5 drops of bromophenol blue, which might have resulted in the indication of the lab.

2. How many “chainobeads” was your enzyme able to make per minute in the 60 – 120 second interval?

20. The -DNA/LB/AMP plate had many transformed colonies of bacteria and they appeared white. The 2nd plate listed had the same except it looked white and exposed to room light and green with a UV light.

D.     Other deadly tests on animals that began to crop up included the Draize Test and another tested called LD50.

The goal of the experiment was to identify Unknown 33A and 33B. Unknown 33A was a white, crystalline solid that had a sweet cherry smell and Unknown 33B was a beige, yellowish color liquid that was translucent and had a viscosity similar to water. Also, the liquid was homogenous and smelled sour, similar to mildew.

Add 5mL of gastric juices (contains both pepsin solution and HCl) to test tube 3

Orange Juice Materials: Three oranges were used for this experiment to obtain a decent amount of juice. Each orange was cut in half and juiced according to steps 1 and 2 of Appendix I of the Laboratory Manual for Biology 201 (Denniston, Wimmers, & Hemm 2015). The juice from the first orange and one half of the second orange was strained into a small beaker following