The experiment was carried out to determine if the malic acid or apple acidity is affected by their ripeness. It is hypothesized that the acidity level of the apple fruit would reduce as the fruit ripens. The experiment was performed by squeezing out the juices from the apple fruit and measuring the juice of the apple with a pH probe. The outcome of the experiment was that the unripe fruits had a high acidic level. The content of malic acid decreases from the unripe fruits until the maturity and the fruit pH fluctuates as it ripens. The pH of the unripe apple was 3.2, with the ripe apple, the pH was 3.9 and with the apples that had time to ripen in the laboratory the pH level was 4.5.
Introduction
The experiment was carried out to determine whether the acidity level of the apple fruit changes as it ripens. Malic acid is predominant in the apple fruit (Etienne et al. 1452). The apple fruit is one of the most preferred fruits around the globe. It has pleasing flavor, sweet taste, flesh and its beautiful appearance attract consumers. The fruit’s attractiveness is usually determined by its elements such as size, appearances, color, uniformity, and its non-visual aspects like favor, firmness, healthiness, nutritional value and taste (Espino-Díaz 376). Besides, aroma and firmness are other essential attributes that appeal to the consumers. The organic acids, sugars mainly contribute to the apple’s aroma. Apple is consumed fresh. The quality of the fruit is determined by its
•In the old days the apple was generally somewhat bitter tasting, so it was used to make alcoholic cider, which in turn got a lot sweeter and fulfilled this “sweetness” we crave. •The apple tree has grown and changed to contain cyanide in the seeds so that the seeds won’t be consumed. This preserves the apple lineage, so that it does not go extinct.
Then the disk should be placed into the hydrogen peroxide solution and times for a reaction. Then another disk is placed into a beaker labeled potato extract for 5 seconds, then placed into the hydrogen peroxide solution and timed for a reaction. The hypothesis for the pH test were correct because the reactions did slow down as the pH got higher. In the data, the average rate of reaction for the highest pH level was 180 seconds or higher. The lowest pH level had the fastest reaction of them all at about 61 seconds.
The purpose of this lab was to figure out which would be a better buffer, lime juice or potato juice? We found through our observations and our data that our hypothesis that potato juice would be a better buffer was incorrect. For example in the beginning of our lime juice and potato juice trial when we mixed hydrochloric acid at zero drops the pH of the lime juice was 3.3 and after 30 drops of hydrochloric acid the pH of the lime juice was 2.6. In comparison the pH of potato juice at the beginning of the hydrochloric acid trial was 7.0, and after 30 drops the pH changed to 4.1. So the difference in pH in the lime juice was less than the pH of potato after 30 drops of the acid. This data proves that our hypothesis that potato juice would be
Purpose/Hypothesis: The purpose of this experiment is to use both cabbage juice and pH paper to determine the pH of household items. This way, we can tell which products are basic and which one are acidic. If we use cabbage juice as an universal pH indicator by comparing it to pH paper then pH determined by the cabbage juice will be unstable because by using cabbage juice, it can be different depending on how diluted it is.
In this experiment, the hypothesis was that if living cells in beef liver is related to the pH of water, then the pH of liver will respond more like water when acid or base is added. According to the data shown above, the pH level declined (from 8 to 1) with the increase number of HCL (acid) drops added to tap water. Whereas the pH level increased ( from 8 to 13) with the increase of NaOH (base) drops added to the tap water. In contrary, the pH recorded in liver did not show this similar pattern as the tap water at all, in fact it showed a constant pH of 7 as drops of HCL and NaOH were added increasingly. The data for liver showed a slight decrease and increase in pH when acid and base was added beyond 20 drops, but was not as significant as tap water. Thus the hypothesis was refuted.
Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.
The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen.
In order for the media to show the change in acidity the solutions are modified and include an indicator chemical. This indicator will change color depending on the ph level of the media it is in. For all the media used in this experiment, the indicator changes to a yellow color when in the presence of an acid and turns magenta/pink when in the presence of a base or alkali.
Catechol, in the presence of oxygen is oxidized by catechol oxidase to form benzoquinone (Harel et al., 1964). Bananas and potatoes contain catechol oxidase that acts on catechol which is initially colorless and converts it to brown (Harel et al., 1964). In this experiment, the effect of pH on the activity of catechol oxidase was conducted using buffers ranging from pH2 to pH10. Two trials were conducted due to the first trial results being altered by an external factor. The results were acquired by taking readings every 2 minutes for 20 minutes from a spectrophotometer and then recorded on to the table. The data collected in the table were then made into graphs to illustrate the influence of pH on the catechol oxidase catalyzed reaction. After analysis, the data revealed that pH did have a significant influence on the enzyme as recorded by absorbance per minute. However, the data was collected was not accurate due to external factors, thus the results are debatable and should be experimented again for validation.
The purpose of this study was to observe the effects of various pHs, temperatures, chemical
The purpose of the experiment was to determine how a buffer works and how to use an acid-base indicator. The way a buffer works was determined by observing the changes in pH of solutions of different concentrations weak acids and their conjugate bases to determine how a buffer affects the pH change. The solution of 10 mL of 0.20 M CH3COOH and 10 mL of 0.20 M CH3COONa had slighter changes in pH than the solution of 10 mL of 0.0020 M CH3COOH and 10 mL of 0.0020 M CH3COONa. Both of these solutions were buffers, shown because they had slighter changes in pH than the solutions with only the weak acid or conjugate base and water. The determination of how buffers work was also tested with observing that the solution of NaC4H3O4 and Na2C4H2O4 had smaller
Malic Acid: Is considered to be safe by the FDA, malic acid was first isolated by Carl Wilhelm Scheele in 1785. This acid is very tart it is most commonly found in green apples and present in wine grapes. One should not consume so much malic acid because it can cause irritation of the
The experiments involved PH buffers of different pH were added to potato juice, water, and the enzyme catecholase. The mixture was then subjected to spectrophotometer at a wavelength of 420nm taking the absorbance readings. In the second experiment, a phosphate buffer of PH 7.0 was used in different measures together with different measurement of potato juice and the enzyme catecholase then subjected to the spectrophotometer at a wavelength of 420nm. The data collected inform of table and analyzed using descriptive statistics such as line graph and later interpreted, showing that PH and enzyme concentration do affect the rate of enzyme reaction
Step 1: Written observation of the symptoms of the fruit and signs of the pathogen.
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