Lab Synthesis Essay

Throughout today’s society, media contributes to almost everyone’s daily life. From informative news channels to comical television shows, media proves to be effective in advertisement, releasing messages and informing the audience. Although media proves to be wildly effective in advertising, releasing messages and informing the audience, periodically destructive and misleading messages are provided to the audience and directly influencing women. Cultural critics widely agree that media tends to negatively influence women and all the critics point to research which supports the belief that women are portrayed as subordinate to men, having no

The purpose of the lab was to determine the ratio of air to acetylene results in complete combustion of acetylene gas. The balanced chemical equation for this experiment was C2H2(g) + O2(g) --> CO2(g)+ H2O(l). Complete combustion is the reaction of an element or compound with oxygen to produce the most common oxides and energy. Complete combustion occurs when the fuel and oxygen combine in exact proportions to completely burn the fuel, which leaves a clean test tube. Incomplete combustion is the reaction of an element or compound with oxygen to produce some oxides with less oxygen than the most common oxides. Incomplete combustion occurs when there is not enough oxygen to react

6. The disks in the 0.00% solution were transferred to an agar plate held next to the blue flame using the sterilized tweezers. Excess disinfectant was removed from the disks by wiping on the side of the well of the spotting tile. When the 5 disks were positioned (refer to Figure 1 below) the lid was replaced and sticky taped down. A label was added indicating the concentration of disinfectant.

Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following:

Negative result is no color change with mineral oil on top of solution. This bacterium has +/- reaction on MAC plate. It could survive in an acidic environment, but it not able to ferment lactose. The bacteria grow on the plate, but did not change the color of the plate to bright pink. This bacterium has a +/- result on Bile Esculin Agar.

20. The -DNA/LB/AMP plate had many transformed colonies of bacteria and they appeared white. The 2nd plate listed had the same except it looked white and exposed to room light and green with a UV light.

14. Use the same loop and technique to spread the same cell suspension (+) on the LB+AMP agar plates. Dispose of the sterile loop in a beaker of germicide.

Lastly, safranin was added to flood the slide and was left on for 1 minute and rinsed with distilled water. After adding all 4 reagents, the slide was blotted dry with a paper towel to remove any excess reagents or water. The slide was then placed on a microscope stage to look for the color of the bacteria. When ready for the highest power on the microscope, immersion oil was added to the slide and looked at under 100x power. A glucose test and citrate test were found to be the best two tests to help narrow down the bacteria.

Lab Day 1: After receiving my unknown bacteria, I streaked a TSA plate and incubated at 37°C for 48 hours. I then picked a single colony from the plate with my sterile loop and streaked a TSA slant and labeled it “Working Stock”. I did the same with another TSA slant and label the second one “Back-up Stock”. This would be the samples I used to complete the following procedures through the next four weeks to determine my unknown bacteria.

A sample was taken, examination under the microscope did reveal some clue cells. An Affirm prep is pending.

To start off unknown #3, I picked out O17 from rack A and wrote down my name, date, class, and I first noticed the broth was a light yellow that was quite cloudy or milky. Soon later I streaked my organism onto a TSA plate and incubated it at 37oC for 24 hours, t was almost a perfect plate. There was no contamination; I could tell it there was only one species on the plate and that it was okay for me to move on. There were many isolated colonies in quadrants 3 and 4. The colonies were small and white in color, it was not translucent. I then began to make my 2 slants, inoculating a straight line on each slant, I incubated it at 37oC for 24 hours. The next day, I made sure my slants were pure and also there was a thick line of organism where I had inoculated with a yellow-white color. Now I was ready to begin making my slides and start staining. I used E. Coli and S. Aureus next to my unknown to help me

The objective of this experiment was to identify two unknown bacteria from a mixed culture. Which was done by using the aseptic technique which was very important to avoid any contamination and keeping the workspace clean while culturing bacteria for different tests. To start, I chose a tube which had a solution of mixed culture. I used the flame to sterilize the inoculating loop and dipped it into the tube and streaked for isolation on 2 TSA plates and placed them in an incubator at 37 for 24 hours. Next day I observed the growth of 2 different types of colonies one for each unknown on the two plates. So I picked the best one and labeled it as master plate and discarded the other plate. From the master plate, I subcultured each type of colony

Figure #1 This picture show the gram stain of unknown #3. The image is under 1000x magnification using oil immersion technique. Gram (-)

In the 10-3 pasteurized sample, the plate exhibited 71,000 cells/mL. The results of the additional dilution samples contained too few colony forming units to count. However, in the 10-7 dilution, although the plate demonstrated 12 colonies, there should have been no colony forming units on this plate. The reasons for this could have been that this sample was contaminated from “double-dipping” the sample before dispensing it onto the plate or when using the pipette, it mistakenly was inserted in a higher concentration sample and then immediately to a lower concentration sample before it was dispensed onto the plate.

The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique is when a cultures has multiple species they are able to be more easily identified once they have been isolated. This experiment is much like the experiments completed on an agar plate but on one a much larger scale and where techniques