Effect of Caffeine on the heart rate of Daphnia
Abstract
In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.
Introduction
Caffeine is a product, which is naturally produced by
Caffeine can be found in a variety of things whether it be; drinks, food, medication etc. There has been several debates on Caffeine and the possible dangerous effects it could have on the heart when consuming too much. In our experiment, we measured the effect that excessive and direct caffeine intake would have on the heart rate of the organism Daphnia Magna. The organism was placed under a microscope, and was then observed prior to and after 5mg/mL of caffeine was introduced into the organism’s environment. After a series of trials the findings had shown that the heart rate of the D. magna did in fact increase when introduced to the 5mg/mL of caffeine. The average heart rate for the controlled being 240 and the average heart rate for the Daphnia Magna exposed to caffeine being 280.8 proves that point. This lead to the conclusion that excess caffeine consumed by an organism can lead to cardiovascular issues such as increased heart rate.
The aim of this experiment was to determine the effect of caffeine on resting body temperature. This has to do with homeostasis, which is the maintenance of equilibrium. In other words, it is a stable body state. Homeostasis refers to the process of keeping the internal body environment in a steady state, when the external environment is changed (Homeostasis 2). Humans are warm-blooded creatures that generate body heat internally and maintain body temperatures at a fairly constant level – about 98.6oF (Chiras 3). In this experiment, the effect of different amounts of caffeine on resting body temperature was examined.
The purpose of this research project is to explore and discover the effect of caffeine on the asexual reproduction of Daphnia magna. It is hypothesized that the more caffeine that is used, the higher the decrease of the asexual
Through the research gathered, the public can understand the risk factors of caffeine and the precautions of overdosing on caffeine. By consuming less caffeine, pregnant women will have a lower chance of miscarrying and the human fetus will grow more healthy. By using zebrafish embryos, scientists are able to examin full embryonic development in a short period of time. Because of the similar structure of fetuses, embryos help scientists understand fetus growth without having to look into a pregnant woman’s stomach. With this data, society will be able to learn the harmful effects of
Sudden cardiac death (SCD) accounts for approximately one half of all cardiac deaths [Zipes and Wellens, 1998], and, although coronary artery disease (CAD) underlies most SCD events, SCD may be the first manifestation of CAD in many individuals, especially women [Albert et al., 2003]. Coffee and other caffeine-containing beverages such as tea, colas, and guarana, a soft drink made from the caffeine-rich seeds of a South American fruit pass certain kind of diseases. Many ills have been ascribed to caffeine, but few have been substantiate [Frost et al., 2005].The effect of caffeine on a disease often associate with caffeine namely, disruption of cardiac rhythm. Arrhythmias are often due to structural heart disease caused by atherosclerosis, hypertension,
In this experiment, the purpose was to identify the effect of sedatives and stimulants on the circulatory system on the ghost shrimp and the black worm. We used caffeine as our stimulant and ethanol as our sedative. The shrimp was placed on a wet petri dish, where it was covered. After letting the shrimp rest for a few minutes, we placed it under the lowest light of a dissecting microscope. Then we located the heart, counted the number of beats in 10 seconds and multiplied it by 6 to acquire the beats-per-minute. In the next trial we dropped 0.5 mL of .0005 M caffeinated solution on the shrimp and waited 2 minutes before we measured the bpm. After the bpm was recorded, the shrimp was washed with spring water. The same procedure was performed
Spriet and Graham,"Caffeine and Exercise Performance”, point out their research on overuse of caffeine. Drugs contain caffeine can cause gastrointestinal discomfort, cramps, insomnia; palpitations should be used in accordance with the prescribed dose. Caffeine effective strengthening of intellectual clarity, and improve thinking ability through blockade of receptors which perform functions adenozyn inform the body needs to sleep. In addition, caffeine also changes some biochemical processes in the body, 2 cups of strong coffee can increase three times the amount of adrenalin in the blood, at the same time accelerate the respiratory rhythm. Caffeine also increase dopamine production stimulating the pleasure centers in the brain, makes people
Caffeine is a stimulant of the nervous system which affects many aspects of the body. The overall effect of caffeine consumption is an overall feeling of awareness and higher levels of energy. Caffeine is the most used drug in the world. From coffee, tea, soda, to daily energy boosters and energy drinks, caffeine has become mainstream in American society, so much so, that over 90% of Americans drink caffeine on an everyday basis (Villanova). This is due to caffeine’s addictive nature. This drug affects almost every aspect of the body in some way, but especially performance. Our experiment is specifically testing the effect of caffeine on athletic performance and intellectual capacity. To test such things on human subjects, it would be very
Caffeine is a plant alkaloid found in many different products. (Archna 2008) Caffeine is categorized as a drug that stimulates the central nervous system and aids in mobilizing free fatty acids which increases the use of fats as an energy source. (Desbrow 2007) This substance when consumed is distributed in intracellular fluid after crossing the blood-brain barrier. This results in
The snails were then placed in Petri dishes to be examined under a microscope to determine their heart rates. After 24 hours, the snails treated with caffeine concentration greater than 0.1% had weaker and irregular heart rates. All the snails that were treated with caffeine solutions 0.5% or 3% were dead after 96 hours.
Caffeine is a plants toxic defense mechanism that is commonly consumed by humans. This caffeine is located in the nectar of plants and is use to prevent herbivores from eating the plant. In this study they observed two caffeine producing plants, Citrus and Coffea in order to provide evidence that the caffeine compound alters pollinator behavior by enhancing their memory due to this “caffeinated award”. They had measured caffeine in nectar of three species of Coffea (C.canephora, C.arabica and C.libercia) and four species of Citrus ( C. paradise, C maxima, C. sinesis and C. reticulata) using liquid chromatography and mass spectroscopy. The median caffeine concentration for both genera was no significantly different P>0, and if present it ranged from 0.003 to 0.253 mM. It was found that caffeine was more common in C.
Male albino Swiss strain of mice were prepared form Razi Institute (Tehran, Iran). We kept animals in the plexiglass cages (5 animals per each cage) on a regular dark/light cycles (12h/12h), controlled temperature (22 ±2 °C) and free access to food and water. We randomly allocated a total of 72 mice into the 9 separate groups (n=8). We used opipramol in 3 different doses (10, 20 and 50 mg/kg), ketamine (50mg/kg), dantrolene (40mg/kg), opipramole (20mg/kg) plus ketamine (50mg/kg), opipramole (20mg/kg) plus dantrolene (40mg/kg), diazepam (5 mg/kg as a positive control) and vehicle 30 minutes before the injection of caffeine. The experiment was approved by the Animal Ethics Committee of the Bushehr University of Medical Sciences, which is in accordance with the European Communities Council to minimize the number and suffering of animals.
Caffeine is a naturally occurring stimulant found in coffee, tea, and chocolate, used as an additive in other energy drinks and acts as analgesic in some pain medications. (Reference) Metabolism of caffeine begins with the absorption of caffeine from consumption of a food or drink product that contains the stimulant, in the small intestine, to then be metabolized in the liver cell and distributed to different parts of the body. Caffeine is metabolized in the liver through an enzyme called cytochrome (CYP1A2). This enzyme controls most of the metabolism process of caffeine. Cytochrome is characterized by its wide range in activity, which is also a result of differences in our genetic makeup. The change in the DNA sequence has a significant
Caffeine is one of the most commonly used drugs. In today’s fast-paced society, lots of people rely on caffeine from coffee to keep them energized throughout the day. Caffeine is a central nervous system stimulant that can give you bursts of energy, but can also make you jittery if too much is consumed. According to the U.S. Food and Drug Administration, about 90 percent of the world’s population ingests some form of caffeine. In the United States, about 80 percent of us take in some caffeine every day. It provides no nutritional value and it has no taste, so it is hard to know if it is in your food (Pietrangelo). Over the years, caffeine consumption has become a controversial subject. Some argue that caffeine consumption can be detrimental to your health, while others say that the effects of caffeine can be beneficial. However, research shows that caffeine is more bad than good. Caffeine consumption can have numerous potentially dangerous effects on a person, both physiologically and psychologically.
Caffeine has become a staple in the majority of the adult population’s normal routine but is the substance actually benefitting one’s mental prowess? Memory is the brain’s ability to catalog skills or experiences for future needs (Sherwood et al. 2005). There are two types of memory which are declarative (explicit) memory and procedural (implicit) memory; declarative memory deals with storage of information dealing with names or places, etc., while procedural memory deals with one’s physiological processes such as walking or talking (Sherwood et al. 2005 ). The physiological condition responsible for the packing of new skills or information is referred to as memory trace, which has two types of storage, short-term or long-term memory. When a new fact or skill is introduced to someone, the first step is to be deposited into short-term memory where it will either soon be discharged or kept within the brain’s internal catalog (long-term memory) through a process known as active practice; the more often a new skill or fact is processed, the more likely the information will be “consolidated” within long-term memory (Sherwood et al. 2005). That information which is stored in long-term memory also faces the possibility of loss due to the significance or use by the individual.