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The Effect Of Peroxidase Production On Stressed Plants Essay

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This experiment used beets to determine peroxidase production in stressed plants. The purpose of this experiment was to test if the stressed plant would produce more peroxidase in a higher concentration than the control plant. The stressor for this experiment was watering with salt water. This stressor was chosen because it is a natural stressor to plants; especially along the coasts and where roads are salted in winter such as the Midwest. The control plant was watered with plain tap water while the experimental plant was watered with tap water plus salt. The salt water concentration was 1.2341 molar. After the two-week stress period the stressed plant was much smaller, wilted, and shriveled compared to the control plant. It also had less water content overall, which made the extraction process somewhat more challenging. To test the peroxidase production in the plants several methods were used, including: tissue printing, DOT-blot assay, electrophoretic analysis, and a spectrophotometric assay. It was concluded that the stressed plant did produce more peroxidase than the control plant and in a higher concentration.
Peroxidase Production in Beets Stressed with Salt Water
There are many types of peroxidases found in plants, animals, and microorganisms. The plant peroxidase that was studied in this lab is the “class III plant peroxidase (POX)” (Hiraga, Sasaki, Ito, Ohashi, & Matsui, 2001). Other studies have made it known “that POXs participate in lignification, suberization,
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