Effect on Peroxidase Activity and Growth Rate of Raphanus sativus L Abstract: The prolific use of antibiotics in agricultural operations is raising concern because it is fed to their livestock, which is then excreted into the soil. The antibiotic concentration in the soil is producing antibiotic resistant bacteria. Plants that get exposed to this type of soil get their biochemistry disrupted. The following study was conducted as an attempt to determine if plant growth rate and peroxidase activity…

Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution, which are coloured, absorb the light depending on how concentrated it is and this produces an electronic reading from…

This experiment used beets to determine peroxidase production in stressed plants. The purpose of this experiment was to test if the stressed plant would produce more peroxidase in a higher concentration than the control plant. The stressor for this experiment was watering with salt water. This stressor was chosen because it is a natural stressor to plants; especially along the coasts and where roads are salted in winter such as the Midwest. The control plant was watered with plain tap water while…

Discussion The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control…

The Enzyme Peroxidase Case Study Lab Report Autumn Vick I. ABSTRACT: The purpose of this experiment is to learn the effects of a certain enzyme (Peroxidase) concentration, to figure out the temperature and pH effects on Peroxidase activity and the effect of an inhibitor. The procedure includes using pH5, H202, Enzyme Extract, and Guaiacol and calibrating a spectrophotometer to determine the effect of enzyme concentration. As the experiment continues, the same reagents are used with the spectrophotometer…

Effect of pH on Enzyme Activity of Peroxidase Thomas Stinde March 15, 2016 Coconino Community College Effect of pH on Enzyme Activity of Peroxidase For us to understand what the effects pH have on enzyme activity of peroxidase, we must first define what an enzyme is and what is done as well as a peroxidase. According to the Oxford Dictionary of English, an enzyme is, “a substance produced by a living organism which acts as a catalyst to bring about a specific biochemical reaction” (Oxford Dictionary…

Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy, speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing the effect…

boiling and concentration on Horseradish Peroxidase ABSTRACT The purpose of this report is to find out the effect of change in the Temperature, PH, boiling, concentration in peroxidase activity. Peroxidase is an enzyme that converts toxic hydrogen peroxide (H2O2) into water and another harmless compound. In this experiment we use, turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change in temperature was highest…

The Effect of Substrate Concentration on Enzymatic Reaction Rate of Turnip Peroxidase Introduction 1. Background: Catalysts are substances that increase the rate of a reaction by providing a different mechanism for the reaction to go through in order to lower the activation energy and are unchanged by the end of the reaction. Enzymes, a specific type of catalyst, are proteins that increase the rate of reaction by converting a substrate to a product in biological processes (Burns, 2002). Enzymes…

In a 1-2 page report, address the following. The first part of your report is the Results section of a scientific paper. In this section, present the results of your experiment without any interpretation. Be sure to adequately answer the following questions: How did the rate of the reaction change as you changed each of the three variables? If you vary the temperature, after a certain temperature, the enzymes will become denatured and then the rate will decrease. Each enzyme…