The Effect of Substrate Concentration on the Rate of Reaction

1144 WordsNov 15, 20085 Pages
Method I generally followed most of my plan for the experiments, but to improve accuracy and reliability, I made one change. In my plan, I said that I was going to use 20cm³ of starch for each experiment. However, in my actual experiment I used 40cm³ of starch. This is because I thought I might not have had enough solution if I only used 20cm³ of starch. If this was the case, then I would have to stop the experiment half way through, which would have prevented me from obtaining accurate results. So for this reason, my results would be more accurate and reliable. For everything else, I followed my original plan. To ensure the results were accurate, I made sure I accurately measured out the amylase and starch solutions for each…show more content…
0.2% starch Time (secs) % Transmission 30 1 60 1 90 30 120 45 150 58 180 70 210 76 240 84 270 90 300 99 0.4% starch Time (secs) % Transmission 30 1 60 1 90 1 120 1 150 19 180 30 210 50 240 55 270 58 300 64 0.5% starch Time (secs) % Transmission 30 1 60 1 90 43 120 57 150 71 180 78 210 83 240 87 270 89 300 90 0.6% starch Time (secs) % Transmission 30 1 60 1 90 14 120 42 150 57 180 63 210 70 240 75 270 77 300 79 0.8% starch Time (secs) % Transmission 30 1 60 1 90 16 120 54 150 71 180 77 210 93 240 94 270 97 300 99 1% starch Time (secs) % Transmission 30 1 60 1 90 17 120 55 150 69 180 76 210 93 240 95 270 97 300 99 I will now plot these results on separate graphs. I have plotted the graphs for the average % transmission against time. I did a separate graph for each concentration of starch. I then worked out the gradient of each curve by drawing a tangent. I worked out the gradient by doing the difference in y divided by the difference in x. The gradient represents the rate of reaction. I will now need to draw a final graph to show concentration of starch against rate of reaction. Below is a table to show the rate of reaction for each
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