The Pathway Of The Enzyme Pathway

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These results shown from this experiment led us to conclude that enzymes work best at certain pH rates. For this particular enzyme, pH 7 worked best. When compared to high levels of pH, the lower levels worked better. The wrong level of pH can denature enzymes; therefore finding the right level is essential. The independent variable was the amount of pH, and the dependent being the rate of oxygen. The results are reliable as they are reinforced by the fact that enzymes typically work best at neutral pH

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2. We measured 1 mL of turnip peroxidase (the enzyme) and 3 mL of neutral buffer (pH corresponding to the test tube number i.e. pH 5 in test tube 5) with a syringe and disposed it into tubes 3, 5, 6, 7, 8, and 10

PURPOSE: Measure the effects of changes in catalase concentration, substrate concentration, and salinity on the reaction rates of an enzyme.

Enzymes are biological catalysts, which accelerate the speed of chemical reactions in the body without being used up or changed in the process. Animals and plants contain enzymes which help break down fats, carbohydrates and proteins into smaller molecules the cells can use to get energy and carry out the processes that allow the plant or animal to survive. Without enzymes, most physiological processes would not take place. Hundreds of different types of enzymes are present in plant and animal cells and each is very specific in its function.

A protein has multiple existing structures, these are the primary, secondary, tertiary and quaternary structures which occur progressively. A protein is essentially a sequence of amino acids which are bonded adjacently, and interact with one another in various ways depending on the R group that the amino acid contains. There are 20 different amino acids which are able to be arranged in any given order, thus giving rise to a potential 2.433x1018 (4.s.f) different combinations, and therefore interactions between the various amino acids.

ith the myosin have been activated by the ATP, they will bind the action binding site.

Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology Department, 2011).

Catabolic pathways release energy by breaking down complex molecules into simpler compounds; and energy is stored in the cell until its needed.

When both NA and Praz act on GPCR in VSM membrane, NA induces production of IP3 and Praz does not. Therefore, to produce same level of contraction from NA only by using NA with Praz, higher concentration of NA is necessary to occupy more receptor. Theoretically, the maximum (100%) concentration should be achieved with high concentration of agonist with antagonist (1 p10). 82.61% of contraction was observed in this experiment. it could be due to tissue damage from experimental process or could be influenced by more than five days storage of prepared tissue (3). 5HT2 receptor is located in the CNS and also located in the periphery (1 p196). The subtypes of 5HT2 are linked to colonic motility (5-HT2A), heart (5-HT2B), and central nervous system (5-HT2c) (1 p196). Meth act as antagonist to 5-HT2A, partial agonist to 5-HT2B, and antagonist to 5-HT2C (1 p199, 4). Both 5HT, and Meth works as agonist, but binding of 5HT to receptor give full response (full agonist), and Meth give less than full response (partial agonist). Meth act as agonist in presence of low concentration of 5HT with Meth. Meth act as antagonist in presence of high concentration of 5HT with Meth. Meth disturbs 5HT binding to receptor to give full response. Therefore, crossover of 5HT only trend line and 5HT with Meth should be observed. Absence of crossover might be due to different efficacies, as Meth is known

Organisms cannot depend solely on spontaneous reactions for the production of materials because they occur slowly and are not responsive to the organism's needs (Martineau, Dean, et al, Laboratory Manual, 43). In order to speed up the reaction process, cells use enzymes as biological catalysts. Enzymes are able to speed up the reaction through lowering activation energy. Additionally, enzymes facilitate reactions without being consumed (manual,43). Each enzyme acts on a specific molecule or set of molecules referred to as the enzyme's substrate and the results of this reaction are called products (manual 43). As a result, enzymes promote a reaction so that substrates are converted into products on a faster pace (manual 43). Most enzymes are proteins whose structure is determined by its sequence of its amino acids. Enzymes are designed to function the best under physiological conditions of PH and temperature. Any change of these variables that change the conformation of the enzyme will destroy or enhance enzyme activity(manual, 43).

Enzymes are central to every biochemical process. Due to their high specificity they are capable of catalyzing hundreds of reactions that signifies their vast practical importance.

Cell use enzymes to speed up a chemical reactions that take places in cells, and enzyme is a protein molecule that has characteristic sequence of amino acids. The amino acids are fold to produce a specific three-dimensional structure, and gives the them unique properties. Another molecule is ribozyme, which is an enzyme made of RNA rather than protein. Enzymes are most likely to be classified by their reaction catalyze.

Enzymes are macromolecules that act as a catalyst, and it’s a chemical agent that accelerates the reaction without being consumed by the feedback or the results (Campbell and Reece, 2005). After the adjustment by the enzymes, the chemical movement through the pathways of metabolism will become awfully crowded because many chemical reactions are taking a long time (Campbell and Reece, 2005). There are two kinds of reactions in nature. The first one is Catabolic reaction and the second one is Anabolic reaction. Catabolic reactions are large molecules that are broken up into smaller molecules (Ahmed, 2013). Anabolic reactions are small molecules that join to make larger molecules, like polymerization (Ahmed, 2013). If you