Essay about Enzyme Lab

1214 WordsNov 22, 20115 Pages
LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable.…show more content…
The human body would not be able to sustain a temperature of 104 degrees F for any length of time. 3. Specifically state where in the intestine sucrase is likely to be most active (pH along GI tract). Small intestine (duodenum) is where the sucrase is likely to be most active. 4. Explain how sucrase activity in the intestine is affected by the sucrose content of food. Why is this important? Sucrase activity in the intestine is affected by sucrose content in food as most foods contain some sucrose. Adding sucrose will increase sucrase activity. 5. Heat treatment was used to denature the sucrase that was added to the control test tube. In the experimental test tube, alkaline DNS was added to denature sucrase to stop the enzymatic reaction. Discuss what temperature was probably used to denature sucrase and what the probable final pH was of each solution after DNS was added. Heat treatment was most likely used as temperatures too high would cause the enzymatic reaction to decrease. The probable temperature that was used would most likely be 70 degrees C. 6. Restate your predictions that were correct and give the data from your experiment that supports them. Restate your predictions that were not correct and correct them, giving the data from your experiment that supports the corrections. I predicted that sucrase will have the greatest activity at pH 6 based on Table 6 of data

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