Introduction The purpose of this lab experiment was to measure the population growth of Escherichia coli, or E. coli, a type of bacteria that can be found in the human body, specifically in the large intestine. It works to aid in digestion as well as the absorption of essential nutrients. While E. coli is extremely beneficial to the human body and most populations are safe for humans, some cause disease if they end up in food that is eaten (Trzepacz et al.) Sickness can occur when waste reaches a water supply, eating meat that is not properly cooked, or drinking milk that is not pasteurized properly. When working with this form of bacteria, it is extremely important to keep it contained for safe measure (Belk et al.) Additionally, this bacterium has also been cited as a cause of infections involving the bloodstream that have life threatening consequences. It is increasingly troubling because antibiotics are now becoming resistant (Collington, 2009.) E. coli is like all other bacteria in the way it carries out reproduction, in that it produces asexually through the process of binary fission. With this being said, the reason why E. coli was chosen specifically is because it grows at a quick pace and is readily available for observation in lab. During this experiment, the population growth of E. coli is measured overtime to observe which conditions allow for the most efficient growth of each culture. It was hypothesized that based upon prior knowledge of this form
Esherichia coli also known as E. coli is a bacterium that lives in your gut. (1). it was founded by Theodore von Esherich in 1888. There are many people that can get the bacteria. Also there are thousands of strands of E.coli. Six E. coli O157 outbreaks were identified during 2007. Four of the outbreaks involved foodborne transmission. (Eshericha Coli). Six Minnesota cases and one Wisconsin case with the same or closely-related PFGE subtype of E. coli O157:H7, and an additional Minnesota case of Shiga-toxin producing E. coli that was not culture-confirmed, attended the Minnesota State Fair in August. All but one of the cases showed cattle or visited the cattle
Since E.coli is found in mammals, studying this subject is important because the factors of E. Coli have risks such as food poisoning. Escherichia Coli is found everywhere! Knowing this, they studied the rapid growth measurements of E.Coli in the lab study. After studying the E. Coli, it was hypothesized that it had a logistic growth overtime. Meaning the growth rapidly grew continuously in size ending with a resting point.
On August 19th, 2015 this experiment was performed, by 6 separate lab groups.The experiment began by measuring 1 Ml of E. coli into a pipette and pump, then placing the bacteria into a culture medium. The E. coli and medium were then swirled together for a period of 15 minutes, until completely mixed. This mixture was then poured into a petri dish and allowed to solidify for 45 minutes. After the 45 minute solidification time, 5 small paper disks were inserted into the dish. 4 of the disk contained treatments of antibiotic and 1 was left untreated. The
E. coli is a bacterium capable of being transmitted from one person to another. To ensure that the bacteria used in this experiment were not transmitted to other places, items, or organisms, several safety protocols were followed.
Escherichia coli are gram negative, rod shaped bacteria that are often used in laboratory studies and are usually harmless, residing in the human digestive tract as part of the normal flora. E. coli 0157:H7 is a pathogenic strain of E. coli that was first identified as a cause of disease in the United States in 1982, during an investigation into an outbreak of gastrointestinal illness. The organism is also called enterohemorrhagic E. coli (EHEC) because it causes abdominal pain with diarrhea that may contain blood. It lives in the intestines of healthy cattle and can contaminate meat during slaughter. It is believed that the widespread use of antibiotics by American farmers has
Escherichia coli, or E. coli, is a common bacterium that can be found in diverse environments all over the planet, including the gastrointestinal tracts of animals and humans. Many of these strains of E. Coli are essential mechanisms in the digestive tract, while others are pathogens that can cause complications in urinary and intestinal tracts. (Payne & Sparks) In research, E. Coli is commonly used as a model organism, meaning they are widely studied by scientists for a variety of purposes due to their experimental advantages. E. Coli is comparatively simple, and there are many advantages to studying these prokaryotic cells in the fields of biochemistry and molecular biology. E. Coli has this simplicity and is relatively easy to propagate in a lab environment. Their genome has been completely sequenced and many things we know about DNA, protein synthesis, and gene linkage have been derived from studies regarding this particular organism. (Cooper)
Escherichia coli (E. coli) is a bacteria usually found in warm blooded organisms such as human and animal intestines. Although there are large groups of E.coli, most strains are harmless. Other strains of the bacteria may cause diarrhea, respiratory illnesses and food poisoning. Some strains such as O157:H7 can cause kidney failure or severe anemia. Exposure to E.coli can be in the form of food, water, undercooked meat and even raw vegetables. Symptoms may include: nausea and vomiting, severe stomach cramps and stomach tenderness, diarrhea containing blood.
In our experiment we are using one of three methods to inject the E.coli bacteria with
The sample tube taken from the pond down by the turf field at USM was taken out of the incubator and observed. This sample was taken February 7th at around 2:30 in the afternoon. The results showed the entire 50ml sample mixed with COLISURE being completely purple, meaning it tested positive for Coliforms. What was different was the 44 wells with the pipetted samples in it. 8 of the wells remained yellow, meaning they were negative for Coliforms and the remaining 36 were all shades of purple meaning they were positive for Coliforms. The well plate along with the 50ml tube of the sample taken from the pond near the turf was then put under an ultra violet light, this helped us detect if the sample was positive for Escherichia coli. Under the ultra violet light, the entire tube sample as well as 3 wells in the well plate were glowing, meaning they were positive for Escherichia coli. In comparison to the other samples, this pond was by far the most dangerous for human consumption or swimming in. After this discovery, the most probable number (MPN) of both positive wells as well as wells positive for Escherichia coli were calculated. For the first sample from the pond near the turf field the MPN was calculated by first identifying the total volume dispensed into the wells. This was calculated was using the equation 0.2ml x 44 wells = 8.8ml. Next, the number of cells per
Escherichia Coli, or E. coli, resides in the large intestine where it assists digestion and make up essential molecules like Vitamin K. For the most part, E. Coli poses no threat although there are few disease causing strains that can cause mild to severe food poisoning by finding its way into food supply (Timmons, Trzepacz, Duboinis-Gray). Enteropathogenic Escherichia coli, or EPEC, is a strain of diseasing causing E. Coli that is the leading cause of death in children with diarrhea in many parts of the world. EPEC colonizes in the intestine allowing the host to be invaded which can spread from person to person (Pearson, Wong fok Lung, Hartland, Giogha). Another strain of disease causing E. Coli also causes diarrhea in children is the DEC
Escherichia coli (E.coli) is a relatively large, rod shaped, gram-negative, fecal coliform most commonly found in the GI tract of warm blooded animals. There are several species of E. coli, most of which are harmless, but some are pathogenic. The most common pathogenic strain is Shiga toxin-producing E. coli, which come from the gut of farm animals and some wild game. Consumption of unpasteurized milk or meat that has not been disinfected spreads the bacterium. Symptoms include stomach cramps, diarrhea, fever and vomiting.
The purpose of this experiment is to show how different concentrations of Ampicillin affect Escherichia Coli Growth and how the bacteria become resistant to the antibiotic drug. Through a series of steps, which involves streaking agar plates with E.coli sample and application of ampicillin to the E.coli sample on the agar plate, the experiment yields a result that supports the hypothesis. The hypothesis acclaims that ampicillin would affect the growth of E.coli; measuring the zone of inhibition approves the claim in the experiment. The measurements of the zone of inhibition indicate that the generation of E.coli expands as the radius reduces. The reduction of the radius shows the E.coli population is reducing and becoming resistant. In other
Escherichia coli is a bacterium that has been important to genetic research because it requires little training to handle, is easily maintained, accessible, and inexpensive (Healy & Livingstone, 2010). Another advantage to using E. coli is their quick doubling time and rapid complementation with their operons. Mutant phenotypes reveal themselves on certain medias. All of these reasons have contributed to why E. coli is so well researched.
E. coli (also known as escherichia coli) is a bacteria that normally lives in the intestines of people and animals named after Dr. Theodor Escherich. Most E. coli are harmless and actually are vital to a healthy human intestinal tract. However, some E. coli are pathogenic, meaning they can cause either diarrhea or illness outside of the intestinal tract(cdc.gov). According to Food Safety.gov, you can get E. coli by eating contaminated food, especially undercooked ground beef,
E.coli O157:H7 bacteria was discovered in 1885 by a German scientist Theodor Escherich, his discovery also found that strands of the bacteria caused infants diarrhea and gastroenteritis which made this an important health discovery.” E. coli is one of the most frequent causes of many common bacterial infections, including cholecystitis bacteremia, cholangitis, urinary tract infection (UTI), and traveler's diarrhea, and other clinical infections such as neonatal meningitis and pneumonia.” Says the food poison journal, All You Need To Know About E. coli. The bacteria was first called bacterium coli but then was changed to Escherichia coli to honor the scientist that discovered it. There are over 700 serotypes of E. coli that are identified and not all of the ones identified are necessarily cause diseases in us humans, some help us and some give us infections. This disease is responsible for a big amount of contaminated foods and drinks. Foods that are