The Presence Of Dna Damage

1704 WordsFeb 28, 20177 Pages
Objectives: We aimed to assess the presence of DNA damage in full term newborns with neonatal sepsis. Materials and Methods: Sixty neonates with early onset neonatal sepsis and 45 apparently healthy controls were enrolled in the study. Screening of neonates were done by modified clinical sepsis score and hematological scoring system , adjusted to the results of blood culture and screening tests. Complete blood count, C-reactive protein and DNA study were done. Results In our study sepsis was likely in 41 patients (68.3%) taking score 3 or 4 with CRP (12 -48 mgL) , Sepsis was very likely in 19 patients (31.7%) taking score ≥ 5 with CRP (48-96 mgL), Sepsis was unlikely in all controls taking score ≤2 .Mean neutrophil count was…show more content…
[3] CRP levels usually begin to rise within 4-6 hours of the onset of sepsis, peak levels reached within 48-72 hrs, and remain elevated until resolution of the inflammatory process. [4]. Neutrophils are the most abundant cells of the innate immune system. When neutrophils are stimulated by microorganisms, they become activated and increase their oxidative metabolism and unorganized release of toxic derivatives that affected many macromolecules including proteins, carbohydrates, lipids and nucleic acid. This results in cell injury and may induce cell death and apoptosis. [5]. Once neutrophils physiological function has been fulfilled in the tissues, they undergo spontaneous apoptosis that occurs to preserve neutrophil membrane integrity in equilibrium between the free radical generation and antioxidant defense [6]. The resolution of inflammation therefore relies on the effective ‘switching off’ mechanism of the neutrophil, the promotion of apoptosis and the successful clearance of these cells. [7] Great interest in research area is directed towards targeting apoptosis by means to treat neutrophil- dominant inflammatory diseases in a timely controlled manner [8,9]. The present study was conducted to assess the DNA damage in newborns with neonatal septicemia. Materials and Methods This study was conducted on 60 full term neonates with early onset sepsis (EOS) ; 40
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